Exam 15: The Future Isnt What It Used to Be: Biotechnology

In muscular dystrophy,muscle cells lack a protein called dystrophin,which leads to weakening of the muscles.As a biomedical researcher,your goal is to create a gene therapy approach to treating muscular dystrophy.You have a sequence of DNA that contains the dystrophin gene and a virus that infects human cells.You want to combine the dystrophin gene with the viral DNA so that the virus can infect and insert the gene into the muscle cells as a way to treat the disease.Explain how you would get the dystrophin gene into the viral DNA.

What property of restriction enzymes allows DNA from different organisms to be combined?

The enzyme EcoRI cuts between the G and the A in the sequence GAATTC.The enzyme BamHI cuts between adjacent G nucleotides in the sequence GGATCC.The sticky ends that are generated by the two enzymes are complementary and will stick together.

Which statement is a characteristic of plasmids?

In PCR,heating of the DNA is necessary because:

In producing Dolly,the first cloned sheep,how many different sheep were involved,either contributing cells or acting as a surrogate mother?

________ involves the fusion of a somatic cell with an enucleated egg cell.

By inserting four developmental genes into ordinary cells such as skin cells,Shinya Yamanaka and James Thomson were able to convert them into:

What is used to cut DNA in generating recombinant DNA?

One advantage of using bacteria in recombinant DNA technology is that they have a ring of DNA that is not part of their chromosome called a:

Forensic DNA typing can prove the innocence of a crime suspect.

Next to plasmids,the most common type of cloning vector is a ________.

Which of the following need to be included in a PCR reaction?

Pigs rich in omega-3 fatty acids:

DNA fragments cut by most restriction enzymes have:

These days many of our food crops are genetically modified.To some people this "frankenfood" poses many dangers and should not be used.Do you agree? Why or why not?

Which is an example of clones that already are produced by nature?

Reproductive cloning has been used to clone humans.

If you compared two samples of DNA at three different locations in the genome and found they had different lengths of short tandem repeats at each location,what could you conclude?

Dolly,the cloned sheep,is the product of which technique?