Question 1

Which of the following statements about NMR spectroscopy are true? Select all that apply.

Accepted Answer

A)  The area under a peak in an NMR spectrum tells us about the number of atoms experiencing the same chemical environment. 
B)  NMR spectroscopy can be used to tell us about any elements in a compound. 
C)  An NMR spectrum tells us about the different isotopes of an element that are present in a compound. 
D)  NMR spectroscopy exploits light in the radiofrequency region of the electromagnetic spectrum. 
A)  The area under a peak in an NMR spectrum tells us about the number of atoms experiencing the same chemical environment. 
B)  NMR spectroscopy can be used to tell us about any elements in a compound. 
C)  An NMR spectrum tells us about the different isotopes of an element that are present in a compound. 
D)  NMR spectroscopy exploits light in the radiofrequency region of the electromagnetic spectrum. A,D

Question 2

Match the size of the following compounds with the order in which they are collected during molecular exclusion chromatography.
-Large

Accepted Answer

A)  First 
B)  Second 
C)  Thired 
A)  First 
B)  Second 
C)  Thired A

Question 3

Match the following techniques with the attributes they exploit to effect separation.
-Isoelectric focusing

Accepted Answer

A)  Electrical charge 
B)  Electrical charge and size 
C)  Size 
A)  Electrical charge 
B)  Electrical charge and size 
C)  Size A

Question 4

A chromatographic technique features which two of the following?

Accepted Answer

A)  Liquid phase 
B)  Stationary phase 
C)  Mobile phase 
D)  Gas phase 
E)  Solid phase 
A)  Liquid phase 
B)  Stationary phase 
C)  Mobile phase 
D)  Gas phase 
E)  Solid phase

Question 5

Which two of the following statements correctly describe gas-liquid chromatography?

Accepted Answer

A)  The mobile phase is a liquid. 
B)  The mobile phase is a gas. 
C)  The stationary phase is a solid. 
D)  The stationary phase is a liquid. 
E)  The stationary phase is a gas. 
A)  The mobile phase is a liquid. 
B)  The mobile phase is a gas. 
C)  The stationary phase is a solid. 
D)  The stationary phase is a liquid. 
E)  The stationary phase is a gas.

Question 6

Match the techniques with the characteristics that they can be used to reveal.
-3-D structure

Accepted Answer

A)  Mass spectrometry 
B)  X-ray crystallography 
C)  Infrared spectroscopy 
D)  NMR spectroscopy 
A)  Mass spectrometry 
B)  X-ray crystallography 
C)  Infrared spectroscopy 
D)  NMR spectroscopy

Question 7

The length of time a compound takes to pass through a chromatographic column is called which one of the following?

Accepted Answer

A)  The holding time 
B)  The release time 
C)  The repulsion time 
D)  The retention time 
E)  The separation time 
A)  The holding time 
B)  The release time 
C)  The repulsion time 
D)  The retention time 
E)  The separation time

Question 8

Rank the following wavelengths according to the energy they represent, with 1 = highest energy, and 3 = lowest energy.

Accepted Answer

a. 1 cm =

Question 9

Which of the following bonds vibrates at the highest frequency in an infrared spectrum? (Carbon has an atomic mass of 12; oxygen has an atomic mass of 16; hydrogen has an atomic mass of 1.)

Accepted Answer

A)  C-H 
B)  O-H 
C)  C-O 
D)  C-C 
E)  O-O 
A)  C-H 
B)  O-H 
C)  C-O 
D)  C-C 
E)  O-O

Question 10

A compound has a distribution ratio of 0.67. When mixed with organic and aqueous solvents, and the mixture allowed to settle, the compound is most likely to partition into the organic phase.

Accepted Answer

A) True 
 B)False

Question 11

Rank the size of the following compounds according to the distance they travel through a gel during electrophoresis, with 1 = travels the furthest distance; 3 = travels the shortest distance.

Accepted Answer

a. Large =

Question 12

Match the size of the following compounds with the order in which they are collected during molecular exclusion chromatography.
-Medium

Accepted Answer

A)  First 
B)  Second 
C)  Thired 
A)  First 
B)  Second 
C)  Thired

Question 13

Match the techniques with the characteristics that they can be used to reveal.
-Identity of functional groups attached to the hydrocarbon framework

Accepted Answer

A)  Mass spectrometry 
B)  X-ray crystallography 
C)  Infrared spectroscopy 
D)  NMR spectroscopy 
A)  Mass spectrometry 
B)  X-ray crystallography 
C)  Infrared spectroscopy 
D)  NMR spectroscopy

Question 14

Rank the following bonds according to the wavelength of infrared light that they absorb, with 1 being the shortest wavelength, and 3 being the longest wavelength.

Accepted Answer

a. C-O = 3

Question 15

Match the following techniques with the attributes they exploit to effect separation.
-SDS-PAGE

Accepted Answer

A)  Electrical charge 
B)  Electrical charge and size 
C)  Size 
A)  Electrical charge 
B)  Electrical charge and size 
C)  Size

Question 16

Which of the following statements about mass spectrometry is true?

Accepted Answer

A)  The species being studied by mass spectrometry must carry a negative charge. 
B)  Mass spectrometry usually generates a mass spectrum with a single peak. 
C)  The molecular ion peak in a mass spectrum can be used to distinguish between structural isomers. 
D)  The sample being studied by mass spectrometry must be a vapour. 
E)  Isotopes of the same element generate the same peak in a mass spectrum. 
A)  The species being studied by mass spectrometry must carry a negative charge. 
B)  Mass spectrometry usually generates a mass spectrum with a single peak. 
C)  The molecular ion peak in a mass spectrum can be used to distinguish between structural isomers. 
D)  The sample being studied by mass spectrometry must be a vapour. 
E)  Isotopes of the same element generate the same peak in a mass spectrum.

Question 17

Which of the following statements regarding chromatography are true? Select all that apply.

Accepted Answer

A)  The mobile phase may be a liquid or a gas. 
B)  The stationary phase is always a solid. 
C)  Separation on the basis of size is called affinity chromatography. 
D)  The fluid exiting the column is called the eluent. 
E)  During chromatography, the mobile phase passes over the stationary phase. 
A)  The mobile phase may be a liquid or a gas. 
B)  The stationary phase is always a solid. 
C)  Separation on the basis of size is called affinity chromatography. 
D)  The fluid exiting the column is called the eluent. 
E)  During chromatography, the mobile phase passes over the stationary phase.

Question 18

The position of a peak in a 13C-NMR spectrum tells us information about which of the following?

Accepted Answer

A)  The molecular mass of the sample being studied. 
B)  The atoms immediately surrounding a carbon atom. 
C)  The number of carbon atoms present in the sample. 
D)  The electrical charge carried by the sample. 
E)  The 3D structure of the sample being studied. 
A)  The molecular mass of the sample being studied. 
B)  The atoms immediately surrounding a carbon atom. 
C)  The number of carbon atoms present in the sample. 
D)  The electrical charge carried by the sample. 
E)  The 3D structure of the sample being studied.

Question 19

Match the following techniques with the attributes they exploit to effect separation.
-2D-PAGE

Accepted Answer

A)  Electrical charge 
B)  Electrical charge and size 
C)  Size 
A)  Electrical charge 
B)  Electrical charge and size 
C)  Size

Question 20

Rank the following wavenumbers according to the energy they represent, with 1 = highest energy, and 3 = lowest energy.

Accepted Answer

a. 2300 cm-1 = 2
b.

Which of the following statements about NMR spectroscopy are true? Select all that apply.

Match the size of the following compounds with the order in which they are collected during molecular exclusion chromatography. -Large

Match the following techniques with the attributes they exploit to effect separation. -Isoelectric focusing

A chromatographic technique features which two of the following?

Which two of the following statements correctly describe gas-liquid chromatography?

Match the techniques with the characteristics that they can be used to reveal. -3-D structure

The length of time a compound takes to pass through a chromatographic column is called which one of the following?

Rank the following wavelengths according to the energy they represent, with 1 = highest energy, and 3 = lowest energy.

Which of the following bonds vibrates at the highest frequency in an infrared spectrum? (Carbon has an atomic mass of 12; oxygen has an atomic mass of 16; hydrogen has an atomic mass of 1.)

A compound has a distribution ratio of 0.67. When mixed with organic and aqueous solvents, and the mixture allowed to settle, the compound is most likely to partition into the organic phase.

Rank the size of the following compounds according to the distance they travel through a gel during electrophoresis, with 1 = travels the furthest distance; 3 = travels the shortest distance.

Match the size of the following compounds with the order in which they are collected during molecular exclusion chromatography. -Medium

Match the techniques with the characteristics that they can be used to reveal. -Identity of functional groups attached to the hydrocarbon framework

Rank the following bonds according to the wavelength of infrared light that they absorb, with 1 being the shortest wavelength, and 3 being the longest wavelength.

Match the following techniques with the attributes they exploit to effect separation. -SDS-PAGE

Which of the following statements about mass spectrometry is true?

Which of the following statements regarding chromatography are true? Select all that apply.

The position of a peak in a ¹³C-NMR spectrum tells us information about which of the following?

Match the following techniques with the attributes they exploit to effect separation. -2D-PAGE

Rank the following wavenumbers according to the energy they represent, with 1 = highest energy, and 3 = lowest energy.

Acids, Bases, and Buffer Solutions: Life in an Aqueous Environment

Kinetics: What Affects the Speed of a Reaction

Equilibria: How Far Do Reactions Go

Energy: What Makes Reactions Go

Reaction Mechanisms: The Chemical Changes That Drive the Chemistry of Life

Chemical Reactions, Oxidation, and Reduction: Bringing Molecules to Life

Metals in Biology: Life Beyond Carbon

Biological Macromolecules: The Infrastructure of Life

Isomerism: Generating Chemical Variety

Molecular Shape and Structure: Life in Three Dimensions

Functional Groups: Adding Function to the Framework of Life

Hydrocarbons: The Framework of Life

Moles, Concentrations, and Dilutions: Making Sense of Chemical Numbers

Molecular Interactions: Holding It All Together

Compounds and Chemical Bonding: Bringing Atoms Together

Atoms: The Foundations of Life

Introduction: Why Biologists Need Chemistry

Filters

Exam 18: Chemical Analysis: Characterizing Chemical Compounds

Which of the following statements about NMR spectroscopy are true? Select all that apply.

Match the size of the following compounds with the order in which they are collected during molecular exclusion chromatography. -Large

Match the following techniques with the attributes they exploit to effect separation. -Isoelectric focusing

A chromatographic technique features which two of the following?

Which two of the following statements correctly describe gas-liquid chromatography?

Match the techniques with the characteristics that they can be used to reveal. -3-D structure

The length of time a compound takes to pass through a chromatographic column is called which one of the following?

Rank the following wavelengths according to the energy they represent, with 1 = highest energy, and 3 = lowest energy.

Which of the following bonds vibrates at the highest frequency in an infrared spectrum? (Carbon has an atomic mass of 12; oxygen has an atomic mass of 16; hydrogen has an atomic mass of 1.)

A compound has a distribution ratio of 0.67. When mixed with organic and aqueous solvents, and the mixture allowed to settle, the compound is most likely to partition into the organic phase.

Rank the size of the following compounds according to the distance they travel through a gel during electrophoresis, with 1 = travels the furthest distance; 3 = travels the shortest distance.

Match the size of the following compounds with the order in which they are collected during molecular exclusion chromatography. -Medium

Match the techniques with the characteristics that they can be used to reveal. -Identity of functional groups attached to the hydrocarbon framework

Rank the following bonds according to the wavelength of infrared light that they absorb, with 1 being the shortest wavelength, and 3 being the longest wavelength.

Match the following techniques with the attributes they exploit to effect separation. -SDS-PAGE

Which of the following statements about mass spectrometry is true?

Which of the following statements regarding chromatography are true? Select all that apply.

The position of a peak in a 13C-NMR spectrum tells us information about which of the following?

Match the following techniques with the attributes they exploit to effect separation. -2D-PAGE

Rank the following wavenumbers according to the energy they represent, with 1 = highest energy, and 3 = lowest energy.

Acids, Bases, and Buffer Solutions: Life in an Aqueous Environment

Kinetics: What Affects the Speed of a Reaction

Equilibria: How Far Do Reactions Go

Energy: What Makes Reactions Go

Reaction Mechanisms: The Chemical Changes That Drive the Chemistry of Life

Chemical Reactions, Oxidation, and Reduction: Bringing Molecules to Life

Metals in Biology: Life Beyond Carbon

Biological Macromolecules: The Infrastructure of Life

Isomerism: Generating Chemical Variety

Molecular Shape and Structure: Life in Three Dimensions

Functional Groups: Adding Function to the Framework of Life

Hydrocarbons: The Framework of Life

Moles, Concentrations, and Dilutions: Making Sense of Chemical Numbers

Molecular Interactions: Holding It All Together

Compounds and Chemical Bonding: Bringing Atoms Together

Atoms: The Foundations of Life

Introduction: Why Biologists Need Chemistry

Filters

The position of a peak in a ¹³C-NMR spectrum tells us information about which of the following?