Question 1

What type of vector is used to produce large amounts of a desired protein in a bacterial cell?

Accepted Answer

A)  cloning vector 
B)  replication vector 
C)  transcription vector 
D)  translation vector 
E)  expression vector 
A)  cloning vector 
B)  replication vector 
C)  transcription vector 
D)  translation vector 
E)  expression vector

Question 2

pUC plasmids are useful for screening cells that contain recombinant DNA because they contain the _____ gene.

Accepted Answer

A)  $\beta$-galactosidase 
B)  tetracycline resistance 
C)  green fluorescent protein 
D)  luciferase 
E)  None of the answers is correct. 
A)  $\beta$-galactosidase 
B)  tetracycline resistance 
C)  green fluorescent protein 
D)  luciferase 
E)  None of the answers is correct.

Question 3

Explain the basis of the polymerase chain reaction.

Accepted Answer

Using primers that flank the sequence of

Question 4

Bacterial plasmid DNA and bacteriophage DNA are commonly used ______________ to introduce foreign DNA into a bacterium.

Accepted Answer

The answer of Bacterial plasmid DNA and bacteriophage DNA are...

Question 5

Each cycle in PCR includes

Accepted Answer

A)  DNA synthesis. 
B)  hybridization of primers. 
C)  strand separation. 
D)  restriction. 
E)  DNA synthesis, hybridization of primers, and strand separation. 
A)  DNA synthesis. 
B)  hybridization of primers. 
C)  strand separation. 
D)  restriction. 
E)  DNA synthesis, hybridization of primers, and strand separation.

Question 6

Briefly outline the steps necessary to create a recombinant DNA molecule.

Accepted Answer

Both the fragment of interest and the ve

Question 7

A pattern of DNA fragments can serve as a _____ of a particular DNA molecule.

Accepted Answer

A)  template 
B)  primer 
C)  fingerprint 
D)  vector 
E)  None of the answers is correct. 
A)  template 
B)  primer 
C)  fingerprint 
D)  vector 
E)  None of the answers is correct.

Question 8

The specificity or stringency of a PCR reaction can be controlled by altering the reaction

Accepted Answer

A)  volume. 
B)  target sequence. 
C)  temperature and salt concentration. 
D)  time. 
E)  None of the answers is correct. 
A)  volume. 
B)  target sequence. 
C)  temperature and salt concentration. 
D)  time. 
E)  None of the answers is correct.

Question 9

The biological role of restriction enzymes in bacteria is to

Accepted Answer

A)  repair DNA. 
B)  induce DNA crossover. 
C)  cleave foreign DNA. 
D)  join gaps in DNA. 
E)  None of the answers is correct. 
A)  repair DNA. 
B)  induce DNA crossover. 
C)  cleave foreign DNA. 
D)  join gaps in DNA. 
E)  None of the answers is correct.

Question 10

What do Southern, northern, and western blots detect, respectively?

Accepted Answer

A)  DNA, RNA, and protein 
B)  DNA, protein, and RNA 
C)  RNA, DNA, and protein 
D)  protein, DNA, and RNA 
E)  RNA, protein, and DNA 
A)  DNA, RNA, and protein 
B)  DNA, protein, and RNA 
C)  RNA, DNA, and protein 
D)  protein, DNA, and RNA 
E)  RNA, protein, and DNA

Exam 5: Exploring Genes and Genomes

What type of vector is used to produce large amounts of a desired protein in a bacterial cell?

pUC plasmids are useful for screening cells that contain recombinant DNA because they contain the _____ gene.

Explain the basis of the polymerase chain reaction.

Bacterial plasmid DNA and bacteriophage DNA are commonly used ______________ to introduce foreign DNA into a bacterium.

Each cycle in PCR includes

Briefly outline the steps necessary to create a recombinant DNA molecule.

A pattern of DNA fragments can serve as a _____ of a particular DNA molecule.

The specificity or stringency of a PCR reaction can be controlled by altering the reaction

The biological role of restriction enzymes in bacteria is to

What do Southern, northern, and western blots detect, respectively?

Biochemistry: an Evolving Science

Protein Composition and Structure

Exploring Proteins and Proteomes

DNA, RNA, and the Flow of Genetic Information

Exploring Evolution and Bioinformatics

Hemoglobin: a Portrait of a Protein in Action

Enzymes: Basic Concepts and Kinetics

Oxidative Phosphorylation

Catalytic Strategies

Regulatory Strategies

Carbohydrates

Lipids and Cell Membranes

Membrane Channels and Pumps

Signal-Transduction Pathways

Metabolism: Basic Concepts and Design

Glycolysis and Gluconeogenesis

The Citric Acid Cycle

The Light Reactions of Photosynthesis

The Calvin Cycle and the Pentose Phosphate Pathway

Glycogen Metabolism

Fatty Acid Metabolism

Protein Turnover and Amino Acid Catabolism

The Biosynthesis of Amino Acids

Nucleotide Biosynthesis

Biosynthesis of Membrane Lipids and Steroids

The Integration of Metabolism

DNA Replication, Repair, and Recombination

Rna Synthesis and Processing

Protein Synthesis

The Control of Gene Expression in Prokaryotes

The Control of Gene Expression in Eukaryotes

Sensory Systems

The Immune System

Molecular Motors

Drug Development

Filters