Exam 21: Molecular Biology Techniques for Cell Biology

Gel electrophoresis separates DNA molecules based on variability in their

A genomic library is a collection of ________ that contains DNA fragments representing most,if not all,of the genome of an organism.

A cDNA library

One of the societal benefits of genetic engineering today is

You have determined the nucleotide and amino acid sequences of a protein involved in feline leukemia.You now want to test the function of this protein in your feline lymphoma cell line.What technique might you use to test the function of the protein?

In molecular biology,the term DNA cloning refers to the process of

Retroviruses and bacteria such as Agrobacterium tumefaciens are commonly used to produce

You have determined the base sequence for a gene from Felis catus (domestic cat)that you believe may be involved in feline leukemia.The nucleotide and probable amino acid sequences are similar to retroviral proteins that become embedded in the host cell membrane.You separate the membrane fraction of feline leukemia cell lysate from the cytoplasmic fraction.What technique could you use to determine if your protein of interest is present in the membrane fraction?

Introducing a foreign piece of DNA into an organism in such a way that it can be passed along through the germ line (eggs and sperm)and hence to subsequent generations creates a ________ organism.

In combination with homologous recombination,the CRISPR/Cas system can be used to accomplish

What new technique or technology may revolutionize the way in which cell biologists alter the genomes of cells and organisms?

Comparing the sequence of ________ in the genomes of many organisms has allowed evolutionary biologists to create a comprehensive "tree" of life showing that every organism falls into one of three domains: Bacteria,Archaea,and Eukarya.

You have discovered a gene that appears to be crucial to the development of the nervous system in C.elegans.Which of the following techniques would allow you to investigate the transcriptional regulation and expression of this gene in vivo?

You wish to investigate proteins from human cells that bind to a specific short sequence of nucleotide bases.Which of the following techniques would allow you to separate proteins from whole cells or complex mixtures based on their ability to bind to a short nucleotide sequence?

You isolate a new bacterium from soil with a revolutionary new culture technique.You are interested in sequencing the genome of this organism using the whole-genome,or shotgun,sequencing approach.Of the following choices,what would be the best way to prepare the genomic DNA for sequencing?

You wish to investigate proteins from human liver cells that bind to a specific short sequence of nucleotide bases you believe to be an enhancer element.Design an experiment that would allow you to isolate and characterize human proteins that bind to the nucleotide sequence you are interested in.(Include at least two separate techniques in your answer.)