Exam 15: Regulation of Gene Expression

A researcher found a method she could use to manipulate and quantify phosphorylation and methylation in embryonic cells in culture.In one set of experiments she succeeded in decreasing methylation of histone tails.Which of the following results would she most likely see?

Which of the following statements correctly describes the role of general transcription factors in eukaryotes?

Gene expression can be blocked by a process called RNA interference.Which of the following molecules is involved in this process?

Gene expression might be altered based on post-transcriptional processing in eukaryotes rather than prokaryotes.Which of the following statements describes this difference?

In the microarray shown in the figure above,if you were searching for genes whose expression was inhibited by hormone treatment,you would search for sequences spotted on the array that showed what level of fluorescence?

Which of the following statements describes one of the functions of the protein called ubiquitin?

In positive control of several sugar-metabolism-related operons,the cAMP receptor protein (CRP)binds to DNA to stimulate transcription.Which of the following events activates CRP?

What would occur if the repressor of an inducible operon were mutated so it could not bind the operator?

If a particular operon encodes enzymes for making an essential amino acid and is regulated like the trp operon,then

You are given an experimental problem involving control of a gene's expression in the embryo of a particular species.One of your first questions is whether the gene's expression is controlled at the level of transcription or translation.Which of the following experiments might best give you an answer?

Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome.If she moves the promoter for the lac operon to the region between the beta galactosidase (lacZ)gene and the permease (lacY)gene,which of the following results would be likely?

A researcher found a method she could use to manipulate and quantify acetylation in embryonic cells in culture.In one set of experiments using this procedure in Drosophila,she was readily successful in increasing acetylation of amino acids in histone tails.Which of the following results would she most likely see?

Which of the following is an example of post-transcriptional control of gene expression?

Which of the following molecules,when taken up by the cell,binds to the repressor so that the repressor no longer binds to the operator?

Researchers are looking for better treatments for breast cancer.For a particular DNA microarray assay (DNA chip),cDNA has been made from the mRNAs of a dozen patients' breast tumor biopsies.What would the researchers be looking for in this type of assay?

Which of the following would not be true of cDNA produced using human brain tissue as the starting material?

What effect does methylation have on DNA?

Which of the following results can a DNA microarray assay detect or identify?

Altering patterns of gene expression in prokaryotes would most likely contribute to the organism's survival in which of the following ways?

Suppose an experimenter becomes proficient with a technique that allows her to move DNA sequences within a prokaryotic genome.If she moves the operator to the far end of the operon,past the transacetylase (lacA)gene,which of the following results would likely occur when the cell is exposed to lactose?