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Question 145

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In the central nervous system, myelin produced by oligodendrocytes (glial cells) functions as an insulating sheath surrounding certain nerve fibers.  To identify candidate genes involved in the myelination process, researchers collected oligodendrocytes from zebrafish and purified the messenger RNA (mRNA) transcripts expressed by these glial cells.  The mRNA was converted to complementary DNA (cDNA) by reverse transcriptase, and the cDNA was fluorescently labeled and assessed for hybridization on a microarray.  The expression of mRNA in other zebrafish cells was similarly measured.Transcripts detected in zebrafish oligodendrocytes at levels greater than 3 times those found in other cells were selected as candidate myelination genes.  Some of the detected genes, including plp1a, Sox10, and mbp, were previously known to be specific to oligodendrocytes, validating the procedure.  The cDNA of newly identified candidates was amplified by PCR, and the ends were digested with the restriction enzymes EcoRI and XhoI.  Genes were then ligated into the multiple cloning site (MCS) of the pSKII vector, shown in Figures 1 and 2, which had also been digested by EcoRI and XhoI.
Passage In the central nervous system, myelin produced by oligodendrocytes (glial cells) functions as an insulating sheath surrounding certain nerve fibers.  To identify candidate genes involved in the myelination process, researchers collected oligodendrocytes from zebrafish and purified the messenger RNA (mRNA) transcripts expressed by these glial cells.  The mRNA was converted to complementary DNA (cDNA) by reverse transcriptase, and the cDNA was fluorescently labeled and assessed for hybridization on a microarray.  The expression of mRNA in other zebrafish cells was similarly measured.Transcripts detected in zebrafish oligodendrocytes at levels greater than 3 times those found in other cells were selected as candidate myelination genes.  Some of the detected genes, including plp1a, Sox10, and mbp, were previously known to be specific to oligodendrocytes, validating the procedure.  The cDNA of newly identified candidates was amplified by PCR, and the ends were digested with the restriction enzymes EcoRI and XhoI.  Genes were then ligated into the multiple cloning site (MCS) of the pSKII vector, shown in Figures 1 and 2, which had also been digested by EcoRI and XhoI. <strong>Figure 1</strong>  Overview of the pSKII vector (plasmid) <strong>Figure 2</strong>  The pSKII MCS, showing the restriction sites of several restriction enzymesTable 1 shows the functional parameters of several restriction enzymes. To confirm that the candidate genes originated in oligodendrocytes, complementary RNA (cRNA) strands were synthesized from the cloned plasmids and hybridized to fixed zebrafish sections.  One of the genes that localized to oligodendrocytes, known as cldnk, produces a 915 base pair mRNA transcript from two exons.  The cldnk gene is involved in tight junction formation and may be required for myelin sheath development around axons. -How would expression of cloned cldnk differ from expression of the endogenous cldnk gene? A) Expression of the cloned gene does not involve splicing whereas expression of the endogenous gene does B) The cloned gene would be expressed at lower levels than the endogenous gene C) The cloned gene would produce a protein with a different sequence than the endogenous gene D) Expression of the cloned gene would produce a different mature mRNA molecule than expression of the endogenous gene Figure 1  Overview of the pSKII vector (plasmid)
Passage In the central nervous system, myelin produced by oligodendrocytes (glial cells) functions as an insulating sheath surrounding certain nerve fibers.  To identify candidate genes involved in the myelination process, researchers collected oligodendrocytes from zebrafish and purified the messenger RNA (mRNA) transcripts expressed by these glial cells.  The mRNA was converted to complementary DNA (cDNA) by reverse transcriptase, and the cDNA was fluorescently labeled and assessed for hybridization on a microarray.  The expression of mRNA in other zebrafish cells was similarly measured.Transcripts detected in zebrafish oligodendrocytes at levels greater than 3 times those found in other cells were selected as candidate myelination genes.  Some of the detected genes, including plp1a, Sox10, and mbp, were previously known to be specific to oligodendrocytes, validating the procedure.  The cDNA of newly identified candidates was amplified by PCR, and the ends were digested with the restriction enzymes EcoRI and XhoI.  Genes were then ligated into the multiple cloning site (MCS) of the pSKII vector, shown in Figures 1 and 2, which had also been digested by EcoRI and XhoI. <strong>Figure 1</strong>  Overview of the pSKII vector (plasmid) <strong>Figure 2</strong>  The pSKII MCS, showing the restriction sites of several restriction enzymesTable 1 shows the functional parameters of several restriction enzymes. To confirm that the candidate genes originated in oligodendrocytes, complementary RNA (cRNA) strands were synthesized from the cloned plasmids and hybridized to fixed zebrafish sections.  One of the genes that localized to oligodendrocytes, known as cldnk, produces a 915 base pair mRNA transcript from two exons.  The cldnk gene is involved in tight junction formation and may be required for myelin sheath development around axons. -How would expression of cloned cldnk differ from expression of the endogenous cldnk gene? A) Expression of the cloned gene does not involve splicing whereas expression of the endogenous gene does B) The cloned gene would be expressed at lower levels than the endogenous gene C) The cloned gene would produce a protein with a different sequence than the endogenous gene D) Expression of the cloned gene would produce a different mature mRNA molecule than expression of the endogenous gene Figure 2  The pSKII MCS, showing the restriction sites of several restriction enzymesTable 1 shows the functional parameters of several restriction enzymes.
Passage In the central nervous system, myelin produced by oligodendrocytes (glial cells) functions as an insulating sheath surrounding certain nerve fibers.  To identify candidate genes involved in the myelination process, researchers collected oligodendrocytes from zebrafish and purified the messenger RNA (mRNA) transcripts expressed by these glial cells.  The mRNA was converted to complementary DNA (cDNA) by reverse transcriptase, and the cDNA was fluorescently labeled and assessed for hybridization on a microarray.  The expression of mRNA in other zebrafish cells was similarly measured.Transcripts detected in zebrafish oligodendrocytes at levels greater than 3 times those found in other cells were selected as candidate myelination genes.  Some of the detected genes, including plp1a, Sox10, and mbp, were previously known to be specific to oligodendrocytes, validating the procedure.  The cDNA of newly identified candidates was amplified by PCR, and the ends were digested with the restriction enzymes EcoRI and XhoI.  Genes were then ligated into the multiple cloning site (MCS) of the pSKII vector, shown in Figures 1 and 2, which had also been digested by EcoRI and XhoI. <strong>Figure 1</strong>  Overview of the pSKII vector (plasmid) <strong>Figure 2</strong>  The pSKII MCS, showing the restriction sites of several restriction enzymesTable 1 shows the functional parameters of several restriction enzymes. To confirm that the candidate genes originated in oligodendrocytes, complementary RNA (cRNA) strands were synthesized from the cloned plasmids and hybridized to fixed zebrafish sections.  One of the genes that localized to oligodendrocytes, known as cldnk, produces a 915 base pair mRNA transcript from two exons.  The cldnk gene is involved in tight junction formation and may be required for myelin sheath development around axons. -How would expression of cloned cldnk differ from expression of the endogenous cldnk gene? A) Expression of the cloned gene does not involve splicing whereas expression of the endogenous gene does B) The cloned gene would be expressed at lower levels than the endogenous gene C) The cloned gene would produce a protein with a different sequence than the endogenous gene D) Expression of the cloned gene would produce a different mature mRNA molecule than expression of the endogenous gene To confirm that the candidate genes originated in oligodendrocytes, complementary RNA (cRNA) strands were synthesized from the cloned plasmids and hybridized to fixed zebrafish sections.  One of the genes that localized to oligodendrocytes, known as cldnk, produces a 915 base pair mRNA transcript from two exons.  The cldnk gene is involved in tight junction formation and may be required for myelin sheath development around axons.
-How would expression of cloned cldnk differ from expression of the endogenous cldnk gene?


A) Expression of the cloned gene does not involve splicing whereas expression of the endogenous gene does
B) The cloned gene would be expressed at lower levels than the endogenous gene
C) The cloned gene would produce a protein with a different sequence than the endogenous gene
D) Expression of the cloned gene would produce a different mature mRNA molecule than expression of the endogenous gene

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