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A Protein Folds Spontaneously in Phosphate-Buffered Saline (PBS)

Question 186

Multiple Choice

A protein folds spontaneously in phosphate-buffered saline (PBS) .  Guanidinium chloride is gradually titrated into the PBS-buffered protein solution, causing the protein to denature.  During the titration, the ΔG° of protein folding is:


A) initially negative and becomes positive.
B) initially positive and becomes negative.
C) initially negative and becomes more negative.
D) initially positive and becomes more positive.

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