A Certain Enzyme Uses Mn²⁺ as a Cofactor Under Physiological

A certain enzyme uses Mn²⁺ as a cofactor under physiological conditions, and scientists hypothesize that Mn²⁺ is required for stabilization of tertiary structure but not for enzyme activity.  Which of the following observations is consistent with this hypothesis?

A) Substitution of Mg²⁺ in place of Mn²⁺ yields a decreased melting temperature and no change in the k_cat of folded proteins.
B) Replacing Mn²⁺ with Cu²⁺ yields an unchanged melting temperature and a decreased k_cat of folded proteins.
C) Using Ni²⁺ in place of Mn²⁺ yields a decreased melting temperature and a k_cat value of zero.
D) Removing Mn²⁺ yields an increased melting temperature and no change in the k_cat of folded proteins.

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