Passage

Passage
Synthesis of coenzyme A (CoA) requires vitamin B₅ as a substrate.  In bacteria, vitamin B₅ is synthesized by condensation of β-alanine and pantoic acid.  β-alanine is produced by decarboxylation of L-aspartate, mediated by the enzyme aspartate α-decarboxylase (ADC) .  Active ADC is formed when its inactive precursor, PanD, binds to a regulatory complex composed of acetyl coenzyme A (AcCoA) and a protein known as PanZ.  Formation of the PanD-PanZ-AcCoA complex causes the activation loop of PanD to fold into a β-pleated sheet, resulting in PanD autocleavage.  Researchers hypothesize that this conformational change allows the Thr57 residue to cleave the N-terminal end of PanD to form ADC.The limited information on the role of PanZ in the synthesis of CoA has stimulated an investigation of its effect on ADC activity.  Escherichia coli cells were provided with varying concentrations of PanZ, and the rate of β-alanine production was measured in three different conditions (Figure 1) .  Reaction vessels were maintained at pH 6 to ensure optimal decarboxylation activity.
Figure 1  Catalytic activity of ADC in E. coli with increasing concentrations of L-aspartate
Adapted from Monteiro DC, Patel V, Bartlett CP, et al. The structure of the PanD/PanZ protein complex reveals negative feedback regulation of pantothenate biosynthesis by coenzyme A. Chem Biol. 2015;22(4) :492-503.
-In E. coli, high levels of CoA lead to decreased β-alanine synthesis.  This is an example of:

A) the competitive inhibition of ADC by PanZ-AcCoA.
B) a positive feedback loop in which CoA stimulates its own synthesis.
C) a negative feedback mechanism driven by AcCoA involving PanZ.
D) the allosteric regulation of PanD by PanZ-AcCoA.

Correct Answer:

Verified

Unlock this answer now
Get Access to more Verified Answers free of charge

Access For Free