A Research Scientist Purifies DNA Polymerase III from Escherichia Coli

A research scientist purifies DNA polymerase III from Escherichia coli extract.  When the enzyme is incubated in a mixture containing DNA templates, RNA primer oligonucleotides, and tagged deoxynucleotides, she finds that the enzyme possesses 3' to 5' exonuclease activity.  Which of the following enzymatic actions was most likely observed during the experiment?

A) Cleavage of DNA strands to remove supercoils
B) Excision of thymine dimers within DNA
C) Removal of mismatched base pairs during DNA replication
D) Removal of RNA primer nucleotides
E) Unwinding of the 2 strands of template DNA

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