Question 1

A patient is receiving treatment for Hodgkin lymphoma. Six weeks after cessation of the treatment, a cytogenetic analysis is performed. A structural aberration is found. Name the most probable aberration(s) and explain how these may occur.

Accepted Answer

The answer of A patient is receiving treatment for Hodgkin...

Question 2

Discuss the impact of FISH technology in hematolymphoid disorders and bone marrow transplant recipients.

Accepted Answer

The answer of Discuss the impact of FISH technology in...

Question 3

Which of the following is an appropriate specimen source for cytogenetic analysis?

Accepted Answer

A)  A source with nucleated cells that can be manipulated into mitosis 
B)  A source with normal constitutive proteins and nucleic acids 
C)  A source of cell aggregates 
D)  Any cell that contains DNA

Question 4

A patient is receiving chemotherapy for Hodgkin lymphoma. When the treatment ends, a cytogenetic analysis is ordered to determine whether the treatment was successful. Which of the following would be the most appropriate specimen to collect for this?

Accepted Answer

A)  Bone marrow 
B)  Lymphoid tissue 
C)  Peripheral blood 
D)  Skin biopsy

Question 5

Which of the following terms is used to describe a chromosomal aberration present in a human cell that is a multiple of n?

Accepted Answer

A)  Trisomy 
B)  Aneuploid 
C)  Polyploidy 
D)  Diploid

Question 6

Which of the following structural aberrations results in loss of an entire chromosomal arm?

Accepted Answer

A)  Inversion 
B)  Deletion 
C)  Isochromosome 
D)  Translocation

Question 7

List at least five indications for performing FISH analysis in patients suspected of having a hematolymphoid disorder.

Accepted Answer

The answer of List at least five indications for performing...

Question 8

The cell cycle contains four phases: G1, S, G2, and mitosis. Which phase must be active to study human chromosomes?

Accepted Answer

A)  S 
B)  G2 
C)  G1 
D)  Mitosis

Question 9

A 3-year-old girl is confirmed to have ALL by immunophenotyping. She is positive for the CALLA antigen. Cytogenetic analysis results indicate the presence of the Philadelphia chromosome. Which of the following applies to her?

Accepted Answer

A)  A poor prognosis 
B)  A good prognosis 
C)  An atypical presentation 
D)  A tyrosine kinase inhibitor response.

Question 10

The chromosome abnormality type that results in a daughter cell with an extra chromosome-or trisomy-or a daughter cell with a loss of chromosome is characterized as which of the following?

Accepted Answer

A)  Constitutional aberration or mosaic 
B)  Numerical aberration 
C)  Structural aberration 
D)  Acquired aberration

Question 11

A laboratory professional should process a bone marrow aspirate for cytogenetic analysis in a patient with a lymphoma by using which of the following?

Accepted Answer

A)  Tissue culture 
B)  Direct harvest 
C)  Suspension tissue culture 
D)  Mitogen stimulation with PHA

Question 12

The presence of a chimerism after a bone marrow transplant indicates:

Accepted Answer

A)  A rejected transplant 
B)  A partial engraftment 
C)  A complete engraftment 
D)  A successful transplant

Question 13

The first abnormality described in the lymphomas was in Burkitt lymphoma. This first demonstration of a proto-oncogene that was translocated to a location known to be active in B-cell lymphoma, resulting in the activation of the oncogene, was at what heavy chain locus?

Accepted Answer

A)  t(12;21) 
B)  t(8;14) 
C)  14q32 
D)  P13;q22

Question 14

Monosomy is an example of:

Accepted Answer

A)  Polyploidy 
B)  Aneuploidy 
C)  Nondisjunction 
D)  Anaphase lag

Exam 41: Chromosome Analysis of Hematopoietic and Lymphoid Disorders