Question 1

A Hodgkin lymphoma patient is admitted for treatment. What would be the benefit in performing an EBV-DNA test on him?

Accepted Answer

A)   It would identify the cause of the Hodgkin lymphoma.
B)   Because the patient is immunocompromised, it would identify an opportunistic pathogen.
C)   It would help monitor the efficacy of his treatment.
D)   It would help rule out other types of lymphomas. C

Question 2

What is the definition of allele?

Accepted Answer

A)   A fundamental substance of heredity that cells use to catalog, express, and propagate information
B)   The two versions of a gene that are inherited from each parent
C)   A messenger molecule that translates the DNA code to induce protein production
D)   The functional unit composed of amino acids B

Question 3

Which molecular technique is useful in monitoring the amount of pathogen-specific nucleic acids in patients with an infectious disease?

Accepted Answer

A)   ASO
B)   FISH
C)   DNA amplification and sequencing
D)   qPCR D

Question 4

Explain the central doctrine of molecular biology using proper nomenclature. Define each phase.

Accepted Answer

The answer of Explain the central doctrine of molecular biology...

Question 5

A 23-year-old female is admitted to the local hospital with a deep vein thrombosis. In addition to initial laboratory workup, which of the following should be investigated as possible causes of the DVT?

Accepted Answer

A)   PCR for sickle cell mutation
B)   FV and prothrombin mutations
C)   BCR/ABL1 fusion gene mutation
D)   TCR gene mutations

Question 6

Explain why molecular testing is essential not only in identifying disorders, such as cancer, but also during the course of a disease.

Accepted Answer

The answer of Explain why molecular testing is essential not...

Question 7

The use of a labeled probe to detect and localize specific RNA or DNA sequences in tissue samples describes which of the following techniques:

Accepted Answer

A)   ASO
B)   PCR
C)   qPCR
D)   FISH

Question 8

Name and compare two molecular methods used to detect mutations associated with sickle cell disease.

Accepted Answer

The answer of Name and compare two molecular methods used...

Question 9

Which of the following is associated with chronic myelogenous leukemia?

Accepted Answer

A)   BCR/ABL1 fusion gene
B)   t(15;17) 
C)   t(8,14) 
D)   PML/RARA fusion gene

Question 10

Explain the impact of a positive test for HTLV-1 in a lymphoma patient.

Accepted Answer

The answer of Explain the impact of a positive test...

Question 11

Minimal residual disease testing is a molecular application for which hematopathology?

Accepted Answer

A)   Leukemia/lymphoma
B)   Sickle cell disease
C)   Cancer
D)   Gene mutations

Question 12

A multiplex reaction that targets conserved regions within the variable (V), diversity (D), and joining (J) regions of the immunoglobulin heavy chain can be used to determine clonality in which neoplasm?

Accepted Answer

A)   CML
B)   AML
C)   B-cell leukemia/lymphoma
D)   Human T-lymphocytic virus type 1

Question 13

Give the advantages of PCR over Southern blot and advantages of Southern blot over PCR.

Accepted Answer

The answer of Give the advantages of PCR over Southern...

Question 14

Which of the following methods would be most useful for identifying point mutations anywhere in the DNA segment?

Accepted Answer

A)   Southern blot analysis
B)   PCR
C)   DNA sequencing
D)   FISH

Question 15

Which of the following assays would be most appropriate for monitoring minimal residual disease?

Accepted Answer

A)   Karyotyping
B)   Southern blot analysis
C)   qPCR

Question 16

In reference to detecting minimal residual disease, which of the following is true?

Accepted Answer

A)   PCR, karyotyping, and Southern blot analysis are equally sensitive and specific.
B)   qPCR is most sensitive for detecting low levels of tumor burden.
C)   Karyotyping is capable of detecting a 0.1% tumor burden.
D)   Southern blot analysis has 100% sensitivity and specificity for detecting aberrant proteins formed as a result of the genetic mutation.

Question 17

Which of the following molecular techniques is useful in detecting the point mutations in a suspected hemoglobinopathy:

Accepted Answer

A)   DNA amplification and sequencing
B)   qPCR
C)   High-resolution melt curve analysis
D)   RT-PCR

Exam 42: Molecular Analysis of Hematologic Diseases