Exam 8: Molecular Biology and Transcription and Rna Processing

Which of the following are present in your liver cells?

What proteins aid in the recognition of the promoter sequence and binding of RNA polymerase II in eukaryotes?

Which type of RNA is translated within a cell?

What proteins bind to silencer sequences, forming a protein "bridge" that bends the DNA over the promoter and prevents transcription?

In a given bacterium, transcription of housekeeping genes is normal, but genes involved in nitrogen metabolism, stress, and chemotaxis are disrupted. Which sigma subunit is intact?

What are the mechanisms for transcription termination in bacteria?

You wish to prevent transcription of all three types of RNA in bacteria. How many different types of RNA polymerase would you need to inhibit?

Polyadenylation begins with the binding of ________ near a six-nucleotide mRNA sequence, AAUAAA, downstream of the stop codon.

If transcription of this gene occurs from left to right in the accompanying diagram, which DNA strand is the coding (non-template)strand?

In humans, the 30S pre-RNA transcript yields three rRNA segments following transcription and RNA cleavage. What RNA transcripts are generated by the 30S pre-RNA transcript in E. coli?

A cell has a defect in polyadenylation. The RNA transcripts encoding which type of protein would not be affected by this defect because they are not polyadenylated?

Which type of RNA interacts with nuclear proteins to form a ribonucleoprotein complex responsible for intron removal?

Describe the three main differences between bacterial and eukaryotic transcripts.

Processing of the 30S pre-RNA transcript in humans produces what three rRNAs after enzymatic cleavage?

If you want to prevent rho-dependent transcription, which sequences would you target to mutate the rho utilization (rut)site?