Question 1

Transcription of inverted repeats produces an mRNA with complementary segments that fold to form what type of secondary structure?

Accepted Answer

The answer of Transcription of inverted repeats produces an mRNA...

Question 2

Using the accompanying diagram, which of the following corresponds to the +1 nucleotide?

Accepted Answer

A) A 
B) B 
C) C 
D) F 
E) G 
A) A 
B) B 
C) C 
D) F 
E) G

Question 3

What is the consensus sequence for the Pribnow box from these sequences?

Accepted Answer

A) TTGATA 
B) TATGAT 
C) TATTAT 
D) ACCA 
E) TAGTAT 
A) TTGATA 
B) TATGAT 
C) TATTAT 
D) ACCA 
E) TAGTAT

Question 4

miRNA is capable of regulating protein production through which process?

Accepted Answer

The answer of miRNA is capable of regulating protein production...

Question 5

How does the antibiotic rifampicin prevent transcription of RNA?

Accepted Answer

A) Rifampicin blocks binding of RNA polymerase to the promoter. 
B) Rifampicin prevents chain elongation by preventing formation of the phosphodiester bond. 
C) Rifampicin prevents the chain termination, so transcripts are too long and are rendered nonfunctional. 
D) Rifampicin blocks ribosomal RNAs from forming functional ribosomes. 
E) Rifampicin blocks translation by preventing the mRNA from being processed. 
A) Rifampicin blocks binding of RNA polymerase to the promoter. 
B) Rifampicin prevents chain elongation by preventing formation of the phosphodiester bond. 
C) Rifampicin prevents the chain termination, so transcripts are too long and are rendered nonfunctional. 
D) Rifampicin blocks ribosomal RNAs from forming functional ribosomes. 
E) Rifampicin blocks translation by preventing the mRNA from being processed.

Question 6

You suspect a region of DNA contains a promoter and wish to confirm that a sequence of interest binds proteins. Which experiment would you use?

Accepted Answer

A) in situ hybridization 
B) band shift assay 
C) pulse-chase assay 
D) Southern blotting 
E) northern blotting 
A) in situ hybridization 
B) band shift assay 
C) pulse-chase assay 
D) Southern blotting 
E) northern blotting

Question 7

Which three types of RNAs are found in both prokaryotes and eukaryotes?

Accepted Answer

mRNA, tRNA

Question 8

Which type of research technique was used to track newly synthesized RNA within a eukaryotic cell?

Accepted Answer

A) in situ hybridization 
B) band shift assay 
C) pulse-chase 
D) Southern blotting 
E) DNA footprint protection assay 
A) in situ hybridization 
B) band shift assay 
C) pulse-chase 
D) Southern blotting 
E) DNA footprint protection assay

Question 9

A gene has acquired a mutation in which the protein product has an "extra" 50 amino acids at the end. Which region of the gene was likely mutated?

Accepted Answer

A) coding region 
B) start codon 
C) promoter sequence 
D) terminator sequence 
E) stop codon 
A) coding region 
B) start codon 
C) promoter sequence 
D) terminator sequence 
E) stop codon

Question 10

Describe how band shift assays and DNA footprint protection assays can be used to identify promoter regions in DNA.

Accepted Answer

A band shift assay uses two identical sa

Question 11

Eukaryotes have how many different RNA polymerases?

Accepted Answer

The answer of Eukaryotes have how many different RNA polymerases?...

Question 12

Name three functional RNAs unique to eukaryotes.

Accepted Answer

snRNA, siR

Question 13

You want to design a drug that prevents transcription of mRNAs but does not affect transcription of other RNAs. What enzyme would you target?

Accepted Answer

A) RNA polymerase I 
B) RNA polymerase II 
C) RNA polymerase III 
D) ribozyme 
E) methyl transferase 
A) RNA polymerase I 
B) RNA polymerase II 
C) RNA polymerase III 
D) ribozyme 
E) methyl transferase

Question 14

Describe the mechanism(s)used by bacteria and eukaryotes for transcription termination.

Accepted Answer

Bacterial transcription termination can

Question 15

Which region(s)of a gene are not found within the mRNA transcript?

Accepted Answer

A) promoter region 
B) termination region 
C) stop codon 
D) promoter and stop codon 
E) promoter and termination region 
A) promoter region 
B) termination region 
C) stop codon 
D) promoter and stop codon 
E) promoter and termination region

Question 16

Which of the following statement(s)accurately describe(s)tRNA?

Accepted Answer

A) It carries amino acids to the ribosome. 
B) It contains codons. 
C) It is transcribed from a DNA template. 
D) all of the above 
E) A and C only 
A) It carries amino acids to the ribosome. 
B) It contains codons. 
C) It is transcribed from a DNA template. 
D) all of the above 
E) A and C only

Question 17

Prokaryotes have a -10 and -35 consensus sequence in their promoter. What are the three eukaryotic promoter sequence elements (or "boxes")?

Accepted Answer

GC-rich, C

Question 18

What is the term for catalytically active RNAs that can activate processes such as self-splicing?

Accepted Answer

The answer of What is the term for catalytically active...

Question 19

What are the two chemical structures found in RNA but not DNA?

Accepted Answer

ribose sug

Question 20

Nucleoli contain many tandem repeat copies of which genes, transcribed by RNA polymerase I?

Accepted Answer

A) mRNA 
B) tRNA 
C) rRNA 
D) siRNA 
E) all of the above 
A) mRNA 
B) tRNA 
C) rRNA 
D) siRNA 
E) all of the above

Exam 8: Molecular Biology and Transcription and Rna Processing

Transcription of inverted repeats produces an mRNA with complementary segments that fold to form what type of secondary structure?

Using the accompanying diagram, which of the following corresponds to the +1 nucleotide?

What is the consensus sequence for the Pribnow box from these sequences?

miRNA is capable of regulating protein production through which process?

How does the antibiotic rifampicin prevent transcription of RNA?

You suspect a region of DNA contains a promoter and wish to confirm that a sequence of interest binds proteins. Which experiment would you use?

Which three types of RNAs are found in both prokaryotes and eukaryotes?

Which type of research technique was used to track newly synthesized RNA within a eukaryotic cell?

A gene has acquired a mutation in which the protein product has an "extra" 50 amino acids at the end. Which region of the gene was likely mutated?

Describe how band shift assays and DNA footprint protection assays can be used to identify promoter regions in DNA.

Eukaryotes have how many different RNA polymerases?

Name three functional RNAs unique to eukaryotes.

You want to design a drug that prevents transcription of mRNAs but does not affect transcription of other RNAs. What enzyme would you target?

Describe the mechanism(s)used by bacteria and eukaryotes for transcription termination.

Which region(s)of a gene are not found within the mRNA transcript?

Which of the following statement(s)accurately describe(s)tRNA?

Prokaryotes have a -10 and -35 consensus sequence in their promoter. What are the three eukaryotic promoter sequence elements (or "boxes")?

What is the term for catalytically active RNAs that can activate processes such as self-splicing?

What are the two chemical structures found in RNA but not DNA?

Nucleoli contain many tandem repeat copies of which genes, transcribed by RNA polymerase I?

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophages

Dna Structure and Replication

The Molecular Biology of Translation

The Integration of Genetic Approaches: Understanding Sickle Cell Disease

Chromosome Structure

Gene Mutation, Dna Repair, and Homologous Recombination

Chromosome Aberrations and Transposition

Regulation of Gene Expression in Bacteria and Bacteriophages

Regulation of Gene Expression in Eukaryotes

Forward Genetics and Recombinant Dna Technology

Applications of Recombinant Dna Technology and Reverse Genetics

Genomics: Genetics From a Whole-Genome Perspective

Cytoplasmic Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution

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