Question 1

Replication moves from the:

Accepted Answer

A)  5` → 3` direction. 
B)  3` → 5` direction. 
C)  5` → 5` direction. 
D)  3` → 3` direction. 
E)  None of the above. 
A)  5` → 3` direction. 
B)  3` → 5` direction. 
C)  5` → 5` direction. 
D)  3` → 3` direction. 
E)  None of the above.

Question 2

Choose the correct answer from the list below. Not all of the answers will be used.
-DNA can serve as a(n) _______________ to direct synthesis of the complementary strand of DNA or RNA.

Accepted Answer

A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki 
A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki

Question 3

What function does helicase perform during replication?

Accepted Answer

A)  It stabilizes the negative charge of the transition state. 
B)  It utilizes ATP to power strand separation. 
C)  It adds negative supercoils to DNA. 
D)  A and C. 
E)  None of the above. 
A)  It stabilizes the negative charge of the transition state. 
B)  It utilizes ATP to power strand separation. 
C)  It adds negative supercoils to DNA. 
D)  A and C. 
E)  None of the above.

Question 4

The free end of the eukaryotic chromosome is thought to form a unique DNA structure that allows for complete replication.This structure involves a G-rich repeating sequence and forms a:

Accepted Answer

A)  replication fork. 
B)  large duplex loop. 
C)  supercoiled loop. 
D)  displacement loop 
E)  None of the above. 
A)  replication fork. 
B)  large duplex loop. 
C)  supercoiled loop. 
D)  displacement loop 
E)  None of the above.

Question 5

What is the difference between Type I and Type II topoisomerase with respect to the thermodynamics of the reactions catalyzed?

Accepted Answer

Type I catalyzes the relaxatio

Question 6

Write the chemical reaction for the addition of the dNTP to a DNA strand at the 3` end.

Accepted Answer

The answer should indicate cle

Question 7

How are breaks sealed in discontinuous DNA formed during replication?

Accepted Answer

DNA ligase is the enzyme that seals the

Question 8

coli, dnaA protein binds to the __________________.

Accepted Answer

origin of

Question 9

Choose the correct answer from the list below. Not all of the answers will be used.
-The strands of DNA must be relaxed or unwound for replication by _______________.

Accepted Answer

A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki 
A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki

Question 10

How is the processivity of DNA polymerase III accomplished?

Accepted Answer

The structure of the enzyme complex allo

Question 11

What is DNA gyrase?

Accepted Answer

A)  a bacterial topoisomerase II 
B)  a bacterial topoisomerase I 
C)  a bacterial helicase 
D)  All of the above. 
E)  None of the above. 
A)  a bacterial topoisomerase II 
B)  a bacterial topoisomerase I 
C)  a bacterial helicase 
D)  All of the above. 
E)  None of the above.

Question 12

During DNA replication, the RNA primer pieces are removed by __________________.

Accepted Answer

The answer of During DNA replication, the RNA primer pieces...

Question 13

How can the leading and lagging strands be synthesized in a coordinated fashion?

Accepted Answer

A)  Specific enzymes control the size of the DNA opening. 
B)  Lagging-strand binding proteins inhibit leading-strand replication if the strands become disproportionate in size. 
C)  Polymerase III is a dimeric holoenzyme, and the looped lagging strand allows the enzyme to proceed in the same direction with each strand. 
D)  All of the above. 
E)  None of the above. 
A)  Specific enzymes control the size of the DNA opening. 
B)  Lagging-strand binding proteins inhibit leading-strand replication if the strands become disproportionate in size. 
C)  Polymerase III is a dimeric holoenzyme, and the looped lagging strand allows the enzyme to proceed in the same direction with each strand. 
D)  All of the above. 
E)  None of the above.

Question 14

Elongation of the DNA strand proceeds in the __________________ direction.

Accepted Answer

The answer of Elongation of the DNA strand proceeds in...

Question 15

Choose the correct answer from the list below. Not all of the answers will be used.
-The type of topoisomerase that does not require ATP is _______________.

Accepted Answer

A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki 
A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki

Question 16

How does the trombone-slide mechanism work to coordinate replication?

Accepted Answer

The mechanism allows a directi

Question 17

The Klenow fragment:

Accepted Answer

A)  has primase activity. 
B)  is an E. coli DNA polymerase fragment. 
C)  has exonuclease activity. 
D)  A and B. 
E)  B and C. 
A)  has primase activity. 
B)  is an E. coli DNA polymerase fragment. 
C)  has exonuclease activity. 
D)  A and B. 
E)  B and C.

Question 18

DNA clamps:

Accepted Answer

A)  are composed of β2 subunits. 
B)  form a ring around the front of the fork of DNA. 
C)  allow the polymerase to move along the DNA molecule without falling off. 
D)  All of the above. 
E)  None of the above. 
A)  are composed of β2 subunits. 
B)  form a ring around the front of the fork of DNA. 
C)  allow the polymerase to move along the DNA molecule without falling off. 
D)  All of the above. 
E)  None of the above.

Question 19

Choose the correct answer from the list below. Not all of the answers will be used.
-The _______________ describes the lengthening of the loop formed by the lagging strand during replication.

Accepted Answer

A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki 
A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki

Question 20

Choose the correct answer from the list below. Not all of the answers will be used.
-_______________ catalyzes the addition of deoxyribonucleotides to DNA.

Accepted Answer

A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki 
A) helicases 
B) Type I 
C) Type II 
D) G-rich strand 
E) telomeres
F)topoisomerases
G)DNA polymerase
H)A-DNA
I)trombone-slide model
J)template
K)Holliday
L)Okazaki

Exam 34: DNA Replication

Replication moves from the:

Choose the correct answer from the list below. Not all of the answers will be used. -DNA can serve as a(n) _______________ to direct synthesis of the complementary strand of DNA or RNA.

What function does helicase perform during replication?

The free end of the eukaryotic chromosome is thought to form a unique DNA structure that allows for complete replication.This structure involves a G-rich repeating sequence and forms a:

What is the difference between Type I and Type II topoisomerase with respect to the thermodynamics of the reactions catalyzed?

Write the chemical reaction for the addition of the dNTP to a DNA strand at the 3` end.

How are breaks sealed in discontinuous DNA formed during replication?

coli, dnaA protein binds to the __________________.

Choose the correct answer from the list below. Not all of the answers will be used. -The strands of DNA must be relaxed or unwound for replication by _______________.

How is the processivity of DNA polymerase III accomplished?

What is DNA gyrase?

During DNA replication, the RNA primer pieces are removed by __________________.

How can the leading and lagging strands be synthesized in a coordinated fashion?

Elongation of the DNA strand proceeds in the __________________ direction.

Choose the correct answer from the list below. Not all of the answers will be used. -The type of topoisomerase that does not require ATP is _______________.

How does the trombone-slide mechanism work to coordinate replication?

The Klenow fragment:

DNA clamps:

Choose the correct answer from the list below. Not all of the answers will be used. -The _______________ describes the lengthening of the loop formed by the lagging strand during replication.

Choose the correct answer from the list below. Not all of the answers will be used. -_______________ catalyzes the addition of deoxyribonucleotides to DNA.

Biochemistry and the Unity of Life

Water, Weak Bonds, and the Generation of Order Out of Chaos

Amino Acids

Protein Three-Dimensional Structure

Techniques in Protein Biochemistry

Basic Concepts of Enzyme Action

Kinetics and Regulation

Mechanisms and Inhibitors

Hemoglobin: an Allosteric Protein

Carbohydrates

Lipids

Membrane Structure and Function

Signal Transduction Pathways

Digestion: Turning a Meal Into Cellular Biochemicals

Metabolism: Basic Concepts and Design

Glycolysis

Gluconeogenesis

Preparation for the Cycle

Harvesting Electrons From the Cycle

The Electron Transport Chain

The Proton-Motive Force

The Light Reactions

The Calvin Cycle

Glycogen Degradation

Glycogen Synthesis

The Pentose Phosphate Pathway

Fatty Acid Degredation

Fatty Acid Synthesis

Lipid Synthesis

Amino Acid Degradation and the Urea Cycle

Amino Acids Synthesis

Nucleotide Metabolism

The Structure of Informational Macromolecules: Dna and Rna

DNA Repair and Recombination

RNA Synthesis and Regulation in Prokaryotes

Gene Expression in Eukaryotes

RNA Processing in Eukaryotes

The Genetic Code

The Mechanism of Protein Synthesis

Recombinant DNA Techniques

Filters