Exam 8: Mechanisms and Inhibitors

An enzyme catalyst mechanism that uses a metal cation to stabilize a negative charge in the active site is ________________.

A ________________ inhibitor binds irreversibly to the active site of an enzyme.

How are the types of inhibition kinetically distinguishable?

How can covalent modification be used to determine the mechanism of action of an enzyme?

Describe the mechanism for the proteolysis catalyzed by chymotrypsin.

Binding of a water molecule to the zinc ion induces:

The initial reaction kinetics of some enzymes results in a quick burst of product in a short period of time, followed by a slower but sustained increase in product formation over time. What does this type of kinetic response tell an enzymologist about the mechanism of the catalysis?

Which of the following curves (no inhibitor, inhibitor 1, or inhibitor 2) represents the rate of reaction versus substrate concentration for a competitive and an uncompetitive inhibitor? Draw the double-reciprocal plot for each case.