Question 1

The Sanger technique for sequencing DNA involves the use of _____________________ nucleotide analogs that terminate chain elongation.

Accepted Answer

2`,3`-dideoxy

Question 2

A technique used to identify RNA after gel electrophoresis and which employs ssDNA in the detection process is:

Accepted Answer

A)  None of the below. 
B)  the Southwestern blot. 
C)  the Western blot. 
D)  the Southern blot. 
E)  the Northern blot. 
A)  None of the below. 
B)  the Southwestern blot. 
C)  the Western blot. 
D)  the Southern blot. 
E)  the Northern blot. E

Question 3

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-The type of vector used for synthesis of protein is _______________.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction D

Question 4

Why are monoclonal antibodies more useful than polyclonal antibodies?

Accepted Answer

Monoclonal antibodies are more specific,

Question 5

Complementary DNA (cDNA) is formed by the action of reverse transcriptase on _____________________.

Accepted Answer

The answer of Complementary DNA (cDNA) is formed by the...

Question 6

Plasmids used in recombinant DNA technology typically:

Accepted Answer

A)  possess a gene for antibiotic resistance. 
B)  replicate independently of the host genome. 
C)  are circular double-stranded molecules. 
D)  All of the above. 
E)  A and B. 
A)  possess a gene for antibiotic resistance. 
B)  replicate independently of the host genome. 
C)  are circular double-stranded molecules. 
D)  All of the above. 
E)  A and B.

Question 7

Describe the technique called a DNA microarray.

Accepted Answer

Oligonucleotides or cDNAs are affixed to

Question 8

PCR is the abbreviation for _____________________, which is an in vitro technique used to make multiple copies of a DNA molecule.

Accepted Answer

polymerase

Question 9

The enzyme _____________________ can be used to add nucleotides to the 3` end of DNA.

Accepted Answer

terminal t

Question 10

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-The enzyme that joins two DNA molecules is _______________.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 11

Explain the basis of the polymerase chain reaction.

Accepted Answer

Using primers that flank the sequence of

Question 12

Which of the following DNA sequences contains a 4-8 base palindromic site? (Note: Only one strand is shown.)

Accepted Answer

A)  CAGTCC 
B)  GCATCC 
C)  CGATTAGC 
D)  GAGAGAGA 
E)  GCATATGC 
A)  CAGTCC 
B)  GCATCC 
C)  CGATTAGC 
D)  GAGAGAGA 
E)  GCATATGC

Question 13

Complementary, single-strand overhangs that are produced by some restriction endonucleases are referred to as _____________________.

Accepted Answer

sticky end

Question 14

Briefly outline the steps necessary to create a recombinant DNA molecule.

Accepted Answer

Both the fragment of interest and the ve

Question 15

Reagents necessary for sequencing by chain termination include:

Accepted Answer

A)  template DNA, deoxyribonucleoside triphosphates (dNTPs), primer, dideoxynucleotide analogs, DNA polymerase, and radioactive probe. 
B)  template DNA, dNTPs, primer, dideoxynucleotide analogs, and DNA polymerase. 
C)  template DNA, dNTPs, primer, dideoxynucleotide analogs, and RNA polymerase. 
D)  All of the above. 
E)  None of the above 
A)  template DNA, deoxyribonucleoside triphosphates (dNTPs), primer, dideoxynucleotide analogs, DNA polymerase, and radioactive probe. 
B)  template DNA, dNTPs, primer, dideoxynucleotide analogs, and DNA polymerase. 
C)  template DNA, dNTPs, primer, dideoxynucleotide analogs, and RNA polymerase. 
D)  All of the above. 
E)  None of the above

Question 16

What is a transcriptome?

Accepted Answer

A transcriptome is the pattern

Question 17

A number of tools are critical to gene exploration. Name at least four.

Accepted Answer

Tools include restriction-enzy

Question 18

For identification of a gene, against which strand of DNA must the probe complement?

Accepted Answer

A)  either strand 
B)  both strands 
C)  only the coding strand 
D)  only the template strand 
E)  None of the above. 
A)  either strand 
B)  both strands 
C)  only the coding strand 
D)  only the template strand 
E)  None of the above.

Question 19

How is a single gene of interest identified on a plate containing many different library clones?

Accepted Answer

By using a probe specific for the DNA of

Question 20

The probe used to isolate a gene from a genomic library is often:

Accepted Answer

A)  the ligand that binds to the protein. 
B)  its promoter region. 
C)  a portion of the mRNA of the gene. 
D)  All of the above. 
E)  None of the above. 
A)  the ligand that binds to the protein. 
B)  its promoter region. 
C)  a portion of the mRNA of the gene. 
D)  All of the above. 
E)  None of the above.

Exam 41: Recombinant Dna Techniques

The Sanger technique for sequencing DNA involves the use of _____________________ nucleotide analogs that terminate chain elongation.

A technique used to identify RNA after gel electrophoresis and which employs ssDNA in the detection process is:

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -The type of vector used for synthesis of protein is _______________.

Why are monoclonal antibodies more useful than polyclonal antibodies?

Complementary DNA (cDNA) is formed by the action of reverse transcriptase on _____________________.

Plasmids used in recombinant DNA technology typically:

Describe the technique called a DNA microarray.

PCR is the abbreviation for _____________________, which is an in vitro technique used to make multiple copies of a DNA molecule.

The enzyme _____________________ can be used to add nucleotides to the 3` end of DNA.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -The enzyme that joins two DNA molecules is _______________.

Explain the basis of the polymerase chain reaction.

Which of the following DNA sequences contains a 4-8 base palindromic site? (Note: Only one strand is shown.)

Complementary, single-strand overhangs that are produced by some restriction endonucleases are referred to as _____________________.

Briefly outline the steps necessary to create a recombinant DNA molecule.

Reagents necessary for sequencing by chain termination include:

What is a transcriptome?

A number of tools are critical to gene exploration. Name at least four.

For identification of a gene, against which strand of DNA must the probe complement?

How is a single gene of interest identified on a plate containing many different library clones?

The probe used to isolate a gene from a genomic library is often:

Biochemistry and the Unity of Life

Water, Weak Bonds, and the Generation of Order Out of Chaos

Amino Acids

Protein Three-Dimensional Structure

Techniques in Protein Biochemistry

Basic Concepts of Enzyme Action

Kinetics and Regulation

Mechanisms and Inhibitors

Hemoglobin: an Allosteric Protein

Carbohydrates

Lipids

Membrane Structure and Function

Signal Transduction Pathways

Digestion: Turning a Meal Into Cellular Biochemicals

Metabolism: Basic Concepts and Design

Glycolysis

Gluconeogenesis

Preparation for the Cycle

Harvesting Electrons From the Cycle

The Electron Transport Chain

The Proton-Motive Force

The Light Reactions

The Calvin Cycle

Glycogen Degradation

Glycogen Synthesis

The Pentose Phosphate Pathway

Fatty Acid Degredation

Fatty Acid Synthesis

Lipid Synthesis

Amino Acid Degradation and the Urea Cycle

Amino Acids Synthesis

Nucleotide Metabolism

The Structure of Informational Macromolecules: Dna and Rna

DNA Replication

DNA Repair and Recombination

RNA Synthesis and Regulation in Prokaryotes

Gene Expression in Eukaryotes

RNA Processing in Eukaryotes

The Genetic Code

The Mechanism of Protein Synthesis

Filters