Question 1

Briefly outline how a cDNA library is made.

Accepted Answer

Reverse transcriptase is used to convert

Question 2

What do Southern, Northern, and Western blots detect, respectively?

Accepted Answer

A)  DNA, RNA, and protein 
B)  DNA, protein, and RNA 
C)  RNA, DNA, and protein 
D)  protein, DNA, and RNA 
E)  RNA, protein, and DNA 
A)  DNA, RNA, and protein 
B)  DNA, protein, and RNA 
C)  RNA, DNA, and protein 
D)  protein, DNA, and RNA 
E)  RNA, protein, and DNA

Question 3

What is a DNA probe?

Accepted Answer

A DNA probe is used to identify specific

Question 4

Describe two ways PCR can be used in medical diagnosis.

Accepted Answer

PCR can be used to detect whet

Question 5

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-A plasmid is an example of a common _______________.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 6

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-DNA sequencing by controlled termination of replication is called the ______________ method.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 7

Bacterial plasmid DNA and bacteriophage DNA are commonly used _____________________ to introduce foreign DNA into a bacterium.

Accepted Answer

The answer of Bacterial plasmid DNA and bacteriophage DNA are...

Question 8

The biological role of restriction enzymes in bacteria is to:

Accepted Answer

A)  repair DNA. 
B)  induce DNA crossover. 
C)  cleave foreign DNA. 
D)  All of the above. 
E)  None of the above. 
A)  repair DNA. 
B)  induce DNA crossover. 
C)  cleave foreign DNA. 
D)  All of the above. 
E)  None of the above.

Question 9

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-_______________is a method for analyzing the expression of thousands of genes.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 10

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-_______________ is the enzyme used to create DNA from RNA.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 11

The specificity or stringency of a PCR reaction can be controlled by altering the reaction:

Accepted Answer

A)  volume. 
B)  target sequence. 
C)  temperature and salt concentration. 
D)  All of the above. 
E)  None of the above. 
A)  volume. 
B)  target sequence. 
C)  temperature and salt concentration. 
D)  All of the above. 
E)  None of the above.

Question 12

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-Instead of radioactivity, current DNA sequencing commonly uses _______________ base analogues.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 13

The technique in which DNA fragments are separated by electrophoresis and identified using a radiolabeled probe is called _____________________.

Accepted Answer

The answer of The technique in which DNA fragments are...

Question 14

What is the basis of the Sanger method?

Accepted Answer

Sanger sequencing is based upon replicat

Question 15

DNA fragments can be visualized in polyacrylamide-gel electrophoresis by staining with _____________________, which binds to double-stranded DNA and fluoresces an intense orange when irradiated by UV light.

Accepted Answer

The answer of DNA fragments can be visualized in polyacrylamide-gel...

Question 16

_____________________ cleave DNA at sites with inverted repeat sequences referred to as palindromic sequences.

Accepted Answer

Restrictio

Question 17

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-DNA sequences are amplified by the _______________.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 18

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-_______________ are enzymes that split DNA at specific base-pair sequences.

Accepted Answer

A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction 
A) ligase 
B) restriction enzymes 
C) DNA microarray 
D) expression 
E) Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Question 19

How is gene disruption used to determine the function of a gene?

Accepted Answer

If a gene is completely disrupted (a pro

Question 20

The enzyme that catalyzes the formation of a phosphodiester linkage at a break in a DNA strand is _____________________.

Accepted Answer

The answer of The enzyme that catalyzes the formation of...

Exam 41: Recombinant Dna Techniques

Briefly outline how a cDNA library is made.

What do Southern, Northern, and Western blots detect, respectively?

What is a DNA probe?

Describe two ways PCR can be used in medical diagnosis.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -A plasmid is an example of a common _______________.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -DNA sequencing by controlled termination of replication is called the ______________ method.

Bacterial plasmid DNA and bacteriophage DNA are commonly used _____________________ to introduce foreign DNA into a bacterium.

The biological role of restriction enzymes in bacteria is to:

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -_______________is a method for analyzing the expression of thousands of genes.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -_______________ is the enzyme used to create DNA from RNA.

The specificity or stringency of a PCR reaction can be controlled by altering the reaction:

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -Instead of radioactivity, current DNA sequencing commonly uses _______________ base analogues.

The technique in which DNA fragments are separated by electrophoresis and identified using a radiolabeled probe is called _____________________.

What is the basis of the Sanger method?

DNA fragments can be visualized in polyacrylamide-gel electrophoresis by staining with _____________________, which binds to double-stranded DNA and fluoresces an intense orange when irradiated by UV light.

_____________________ cleave DNA at sites with inverted repeat sequences referred to as palindromic sequences.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -DNA sequences are amplified by the _______________.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -_______________ are enzymes that split DNA at specific base-pair sequences.

How is gene disruption used to determine the function of a gene?

The enzyme that catalyzes the formation of a phosphodiester linkage at a break in a DNA strand is _____________________.

Biochemistry and the Unity of Life

Water, Weak Bonds, and the Generation of Order Out of Chaos

Amino Acids

Protein Three-Dimensional Structure

Techniques in Protein Biochemistry

Basic Concepts of Enzyme Action

Kinetics and Regulation

Mechanisms and Inhibitors

Hemoglobin: an Allosteric Protein

Carbohydrates

Lipids

Membrane Structure and Function

Signal Transduction Pathways

Digestion: Turning a Meal Into Cellular Biochemicals

Metabolism: Basic Concepts and Design

Glycolysis

Gluconeogenesis

Preparation for the Cycle

Harvesting Electrons From the Cycle

The Electron Transport Chain

The Proton-Motive Force

The Light Reactions

The Calvin Cycle

Glycogen Degradation

Glycogen Synthesis

The Pentose Phosphate Pathway

Fatty Acid Degredation

Fatty Acid Synthesis

Lipid Synthesis

Amino Acid Degradation and the Urea Cycle

Amino Acids Synthesis

Nucleotide Metabolism

The Structure of Informational Macromolecules: Dna and Rna

DNA Replication

DNA Repair and Recombination

RNA Synthesis and Regulation in Prokaryotes

Gene Expression in Eukaryotes

RNA Processing in Eukaryotes

The Genetic Code

The Mechanism of Protein Synthesis

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