Question 1

How does puromycin inhibit protein synthesis?

Accepted Answer

Puromycin resembles an amino acid attach

Question 2

Which of the following E. coli proteins is responsible for translocation of the mRNA as a result of a conformation change due to the hydrolysis of GTP?

Accepted Answer

A)  RRF 
B)  EF-Tu 
C)  EF-G 
D)  Shine-Delgarno 
E)  None of the above. 
A)  RRF 
B)  EF-Tu 
C)  EF-G 
D)  Shine-Delgarno 
E)  None of the above.

Question 3

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-The protein _______________ is toxic because it hydrolyzes a critical adenine base from the 28S RNA molecule of eukaryotes.

Accepted Answer

A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP 
A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP

Question 4

The signal sequence that marks a protein for translocation across the endoplasmic reticulum membrane is located near the __________________ end of the protein.

Accepted Answer

N-terminal

Question 5

In vanishing white matter disease, nerve cells disappear due to:

Accepted Answer

A)  phagocytosis of living cells. 
B)  an autoimmune result of mutations in mRNA. 
C)  mutation in release factors. 
D)  mutations in initiation factor 2. 
E)  mutations in the tRNA charging mechanism. 
A)  phagocytosis of living cells. 
B)  an autoimmune result of mutations in mRNA. 
C)  mutation in release factors. 
D)  mutations in initiation factor 2. 
E)  mutations in the tRNA charging mechanism.

Question 6

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-The _______________ proteins recognize UAA, UAG, and UGA codons.

Accepted Answer

A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP 
A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP

Question 7

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-_______________ inhibits the interaction between tRNA and 16S rRNA

Accepted Answer

A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP 
A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP

Question 8

Eukaryotic mRNA is:

Accepted Answer

A)  linear. 
B)  circular. 
C)  modified only at the ribose. 
D)  discontinuously translated. 
E)  None of the above. 
A)  linear. 
B)  circular. 
C)  modified only at the ribose. 
D)  discontinuously translated. 
E)  None of the above.

Question 9

Can a protein fully translated and released from a ribosome enter the mitochondria or ER lumen?

Accepted Answer

No, entry into the mitochondri

Question 10

What is the function of RF1?

Accepted Answer

RF1 is a release factor that r

Question 11

GTP binds to the __________________ protein in the translocation mechanism.

Accepted Answer

The answer of GTP binds to the __________________ protein in...

Question 12

mRNA that codes for more than one protein is considered __________________.

Accepted Answer

The answer of mRNA that codes for more than one...

Question 13

What is an iron response element?

Accepted Answer

An IRE is a 5` untranslated st

Question 14

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-_______________ is a sequence of 9-12 hydrophobic amino acid residues, sometimes containing positively charged amino acids.

Accepted Answer

A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP 
A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP

Question 15

Release factors function by allowing __________________ to attack the ester linkage between tRNA and amino acid.

Accepted Answer

The answer of Release factors function by allowing __________________ to...

Question 16

Eukaryotic and prokaryotic translation differs primarily in which step?

Accepted Answer

A)  initiation 
B)  elongation 
C)  termination 
D)  All of the above. 
E)  None of the above. 
A)  initiation 
B)  elongation 
C)  termination 
D)  All of the above. 
E)  None of the above.

Question 17

Which of the following is/are required for the initiation of protein synthesis in prokaryotes?

Accepted Answer

A)  mRNA 
B)  the 30S subunit 
C)  fMet-tRNAf 
D)  GTP 
E)  All of the above. 
A)  mRNA 
B)  the 30S subunit 
C)  fMet-tRNAf 
D)  GTP 
E)  All of the above.

Question 18

What are the names for the tRNA binding sites on the ribosome?

Accepted Answer

They are called the

Question 19

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-How many release factors do eukaryotes have?

Accepted Answer

A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP 
A) initiation factors 
B) ATP 
C) ricin 
D) one 
E) Shine-Dalgarno
F)elongation factor Tu
G)polysome
H)two
I)kanamycin
J)release factor
K)N-formylmethionine
L)signal sequence
M)GTP

Question 20

Describe the subunits of E. coli ribosomes.

Accepted Answer

E. coli ribosomes contain a large (50S)

Exam 40: The Mechanism of Protein Synthesis

How does puromycin inhibit protein synthesis?

Which of the following E. coli proteins is responsible for translocation of the mRNA as a result of a conformation change due to the hydrolysis of GTP?

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -The protein _______________ is toxic because it hydrolyzes a critical adenine base from the 28S RNA molecule of eukaryotes.

The signal sequence that marks a protein for translocation across the endoplasmic reticulum membrane is located near the __________________ end of the protein.

In vanishing white matter disease, nerve cells disappear due to:

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -The _______________ proteins recognize UAA, UAG, and UGA codons.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -_______________ inhibits the interaction between tRNA and 16S rRNA

Eukaryotic mRNA is:

Can a protein fully translated and released from a ribosome enter the mitochondria or ER lumen?

What is the function of RF1?

GTP binds to the __________________ protein in the translocation mechanism.

mRNA that codes for more than one protein is considered __________________.

What is an iron response element?

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -_______________ is a sequence of 9-12 hydrophobic amino acid residues, sometimes containing positively charged amino acids.

Release factors function by allowing __________________ to attack the ester linkage between tRNA and amino acid.

Eukaryotic and prokaryotic translation differs primarily in which step?

Which of the following is/are required for the initiation of protein synthesis in prokaryotes?

What are the names for the tRNA binding sites on the ribosome?

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -How many release factors do eukaryotes have?

Describe the subunits of E. coli ribosomes.

Biochemistry and the Unity of Life

Water, Weak Bonds, and the Generation of Order Out of Chaos

Amino Acids

Protein Three-Dimensional Structure

Techniques in Protein Biochemistry

Basic Concepts of Enzyme Action

Kinetics and Regulation

Mechanisms and Inhibitors

Hemoglobin: an Allosteric Protein

Carbohydrates

Lipids

Membrane Structure and Function

Signal Transduction Pathways

Digestion: Turning a Meal Into Cellular Biochemicals

Metabolism: Basic Concepts and Design

Glycolysis

Gluconeogenesis

Preparation for the Cycle

Harvesting Electrons From the Cycle

The Electron Transport Chain

The Proton-Motive Force

The Light Reactions

The Calvin Cycle

Glycogen Degradation

Glycogen Synthesis

The Pentose Phosphate Pathway

Fatty Acid Degredation

Fatty Acid Synthesis

Lipid Synthesis

Amino Acid Degradation and the Urea Cycle

Amino Acids Synthesis

Nucleotide Metabolism

The Structure of Informational Macromolecules: Dna and Rna

DNA Replication

DNA Repair and Recombination

RNA Synthesis and Regulation in Prokaryotes

Gene Expression in Eukaryotes

RNA Processing in Eukaryotes

The Genetic Code

Recombinant Dna Techniques

Filters