Question 1

What technique distributes the contents of a homogenate into various layers according to the components' densities?

Accepted Answer

A) sucrose density gradient centrifugation 
B) sucrose density chromatography 
C) isoelectric density focusing 
D) liquid scintillation spectrometry 
E) autoradiography 
A) sucrose density gradient centrifugation 
B) sucrose density chromatography 
C) isoelectric density focusing 
D) liquid scintillation spectrometry 
E) autoradiography

Question 2

Which technique depends on the use of antibodies made specifically against a particular protein (antigen)? The antibodies are then conjugated to a substance that makes them visible under the light or electron microscope.

Accepted Answer

A) immunolocalization 
B) immunocompetence 
C) immunization 
D) immunofenestration 
E) negative staining 
A) immunolocalization 
B) immunocompetence 
C) immunization 
D) immunofenestration 
E) negative staining

Question 3

What word or phrase describes what you get if the magnification power of the microscope exceeds the ability of the scope to resolve?

Accepted Answer

A) empty resolution 
B) waste 
C) empty magnification 
D) refractibility 
E) birefringence 
A) empty resolution 
B) waste 
C) empty magnification 
D) refractibility 
E) birefringence

Question 4

Antibodies made by a clone of antibody-producing cells derived from a single B lymphocyte have _________; each different B cell has membrane-bound antibodies with an affinity for __________.

Accepted Answer

A) identical antigen-combining sites,a different part of the antigen 
B) variable antigen-combining sites,the same part of the antigen 
C) identical antigen-combining sites,the same part of the antigen 
D) variable antigen-combining sites,a different part of the antigen 
E) different antigen-binding sites,the cell membrane 
A) identical antigen-combining sites,a different part of the antigen 
B) variable antigen-combining sites,the same part of the antigen 
C) identical antigen-combining sites,the same part of the antigen 
D) variable antigen-combining sites,a different part of the antigen 
E) different antigen-binding sites,the cell membrane

Question 5

The amount of light passing through a solution unabsorbed or _________ is measured by ________ on the other side of the cuvette.

Accepted Answer

A) transmitted light,thermocouplers 
B) absorbed light,thermocouplers 
C) transmitted light,photocells 
D) absorbed light,photocells 
E) transmitted light,charged coupling devices 
A) transmitted light,thermocouplers 
B) absorbed light,thermocouplers 
C) transmitted light,photocells 
D) absorbed light,photocells 
E) transmitted light,charged coupling devices

Question 6

How do you prepare tissue for electron microscopy without using fixatives?

Accepted Answer

A) Freeze it gradually over a few hours. 
B) Freeze it rapidly. 
C) Subject it to a vacuum rapidly. 
D) Heat it to dry it rapidly. 
E) Coat it quickly in heavy metal. 
A) Freeze it gradually over a few hours. 
B) Freeze it rapidly. 
C) Subject it to a vacuum rapidly. 
D) Heat it to dry it rapidly. 
E) Coat it quickly in heavy metal.

Question 7

How do DNA molecules place themselves in a CsCl equilibrium gradient?

Accepted Answer

A) They continuously move toward the bottom of the gradient. 
B) They stop momentarily at the point in the gradient equaling their buoyant density. 
C) They continuously move toward the top of the gradient. 
D) They stop permanently at the point in the gradient equaling their buoyant density. 
E) All DNA molecules localize at the same point in the gradient. 
A) They continuously move toward the bottom of the gradient. 
B) They stop momentarily at the point in the gradient equaling their buoyant density. 
C) They continuously move toward the top of the gradient. 
D) They stop permanently at the point in the gradient equaling their buoyant density. 
E) All DNA molecules localize at the same point in the gradient.

Question 8

A(n)________ is a substance that reveals its presence in one way or another and thus can be localized or monitored during an experiment.

Accepted Answer

A) trancer 
B) tracer 
C) phosphor 
D) fluor 
E) trancept 
A) trancer 
B) tracer 
C) phosphor 
D) fluor 
E) trancept

Question 9

During the cloning procedure,what is the purpose of inserting genes into a bacterium that give it resistance to antibiotics?

Accepted Answer

A) Antibiotic resistance allows researchers to select for those cells that contain a recombinant plasmid. 
B) Antibiotic resistance allows researchers to select for mutations they want to introduce. 
C) Antibiotic resistance allows researchers to kill those cells that contain a recombinant plasmid. 
D) Antibiotic resistance increases the generation time of the bacteria. 
E) Antibiotic resistance decreases bacterial generation time. 
A) Antibiotic resistance allows researchers to select for those cells that contain a recombinant plasmid. 
B) Antibiotic resistance allows researchers to select for mutations they want to introduce. 
C) Antibiotic resistance allows researchers to kill those cells that contain a recombinant plasmid. 
D) Antibiotic resistance increases the generation time of the bacteria. 
E) Antibiotic resistance decreases bacterial generation time.

Question 10

What is the name of a piece of DNA containing sequences derived from more than one organism?

Accepted Answer

A) combinational DNA 
B) recombinant DNA 
C) combined DNA 
D) cloned DNA 
E) recreational DNA 
A) combinational DNA 
B) recombinant DNA 
C) combined DNA 
D) cloned DNA 
E) recreational DNA

Question 11

In which technique is radioactivity detected by mixing the sample in a vial with a special fluid that contains compounds that emit light if struck by a beta particle? The amount of light emitted in the fluid is a measure of the amount of radioactivity in sample.

Accepted Answer

A) autoradiography 
B) an NMR machine 
C) liquid scintillation spectrometry 
D) mass spectrometry 
E) AFM 
A) autoradiography 
B) an NMR machine 
C) liquid scintillation spectrometry 
D) mass spectrometry 
E) AFM

Question 12

What does cloning of cDNAs allow?

Accepted Answer

A) the determination of all genes present in a cell 
B) the identification of sequences active at given time in a particular cell type 
C) the identification of genes active throughout an organism's body 
D) the sequence of all genes present in a given cell 
E) the sequences of all cellular introns 
A) the determination of all genes present in a cell 
B) the identification of sequences active at given time in a particular cell type 
C) the identification of genes active throughout an organism's body 
D) the sequence of all genes present in a given cell 
E) the sequences of all cellular introns

Question 13

A plant cell that lacks a cell wall is called a(n)_________.

Accepted Answer

A) celluplast 
B) protoblast 
C) protoplast 
D) spheroplast 
E) epiblast 
A) celluplast 
B) protoblast 
C) protoplast 
D) spheroplast 
E) epiblast

Question 14

How are probes labeled with a radioactive isotope detected?

Accepted Answer

A) autoradiography 
B) fluorescence 
C) by changing colors 
D) SEM 
E) TEM 
A) autoradiography 
B) fluorescence 
C) by changing colors 
D) SEM 
E) TEM

Question 15

What is defined as viral-mediated gene transfer,which is a very widely used strategy to get genes into eukaryotic cells?

Accepted Answer

A) transduction 
B) transfection 
C) electroporation 
D) lipofection 
E) transgenics 
A) transduction 
B) transfection 
C) electroporation 
D) lipofection 
E) transgenics

Question 16

__________ are established with cells that have been obtained directly from the organism; most of these cultures produced from animal cells are obtained from ________.

Accepted Answer

A) Primary cultures,embryos 
B) Secondary cultures,adult organisms 
C) Primary cultures,adult organisms 
D) Secondary cultures,embryos 
E) Secondary cultures,primary cultures 
A) Primary cultures,embryos 
B) Secondary cultures,adult organisms 
C) Primary cultures,adult organisms 
D) Secondary cultures,embryos 
E) Secondary cultures,primary cultures

Question 17

Whole blood from an animal that has been exposed to foreign materials (antigens)and then had the cells and clotting factors removed from it is called _________.

Accepted Answer

A) antibodies 
B) antigenic syrup 
C) antiserum 
D) immune serum 
E) serosal secretions 
A) antibodies 
B) antigenic syrup 
C) antiserum 
D) immune serum 
E) serosal secretions

Question 18

Which part of a microscope is responsible for gathering diffuse rays from the microscope light source and illuminating the specimen with a small cone of bright light?

Accepted Answer

A) condenser lens 
B) objective lens 
C) ocular lens 
D) iris 
E) focusing knob 
A) condenser lens 
B) objective lens 
C) ocular lens 
D) iris 
E) focusing knob

Question 19

What does the change in field direction in pulsed field electrophoresis accomplish?

Accepted Answer

A) It makes the DNA molecules move faster. 
B) It causes the DNA molecules to reorient themselves during their migration. 
C) It causes the DNA molecules to denature. 
D) It causes the DNA molecules to renature. 
E) It causes the stain to adhere better to the DNA molecules. 
A) It makes the DNA molecules move faster. 
B) It causes the DNA molecules to reorient themselves during their migration. 
C) It causes the DNA molecules to denature. 
D) It causes the DNA molecules to renature. 
E) It causes the stain to adhere better to the DNA molecules.

Question 20

The magnification of a microscope is equal to __________.

Accepted Answer

A) the product of magnification produced individually by the ocular and the N.A. 
B) the product of magnification produced individually by the ocular and objective lenses 
C) the magnification power of the objective lens divided by the N.A. 
D) the magnification power of the ocular lens divided by the N.A. 
E) the magnification power of the objective lens divided by that of the ocular lens. 
A) the product of magnification produced individually by the ocular and the N.A. 
B) the product of magnification produced individually by the ocular and objective lenses 
C) the magnification power of the objective lens divided by the N.A. 
D) the magnification power of the ocular lens divided by the N.A. 
E) the magnification power of the objective lens divided by that of the ocular lens.

What technique distributes the contents of a homogenate into various layers according to the components' densities?

Which technique depends on the use of antibodies made specifically against a particular protein (antigen)? The antibodies are then conjugated to a substance that makes them visible under the light or electron microscope.

What word or phrase describes what you get if the magnification power of the microscope exceeds the ability of the scope to resolve?

Antibodies made by a clone of antibody-producing cells derived from a single B lymphocyte have _; each different B cell has membrane-bound antibodies with an affinity for __.

The amount of light passing through a solution unabsorbed or _ is measured by on the other side of the cuvette.

How do you prepare tissue for electron microscopy without using fixatives?

How do DNA molecules place themselves in a CsCl equilibrium gradient?

A(n)________ is a substance that reveals its presence in one way or another and thus can be localized or monitored during an experiment.

During the cloning procedure,what is the purpose of inserting genes into a bacterium that give it resistance to antibiotics?

What is the name of a piece of DNA containing sequences derived from more than one organism?

In which technique is radioactivity detected by mixing the sample in a vial with a special fluid that contains compounds that emit light if struck by a beta particle? The amount of light emitted in the fluid is a measure of the amount of radioactivity in sample.

What does cloning of cDNAs allow?

A plant cell that lacks a cell wall is called a(n)_________.

How are probes labeled with a radioactive isotope detected?

What is defined as viral-mediated gene transfer,which is a very widely used strategy to get genes into eukaryotic cells?

__ are established with cells that have been obtained directly from the organism; most of these cultures produced from animal cells are obtained from .

Whole blood from an animal that has been exposed to foreign materials (antigens)and then had the cells and clotting factors removed from it is called _________.

Which part of a microscope is responsible for gathering diffuse rays from the microscope light source and illuminating the specimen with a small cone of bright light?

What does the change in field direction in pulsed field electrophoresis accomplish?

The magnification of a microscope is equal to __________.

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics,enzymes and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters

Exam 18: Techniques in Cell and Molecular Biology

What technique distributes the contents of a homogenate into various layers according to the components' densities?

Which technique depends on the use of antibodies made specifically against a particular protein (antigen)? The antibodies are then conjugated to a substance that makes them visible under the light or electron microscope.

What word or phrase describes what you get if the magnification power of the microscope exceeds the ability of the scope to resolve?

Antibodies made by a clone of antibody-producing cells derived from a single B lymphocyte have _________; each different B cell has membrane-bound antibodies with an affinity for __________.

The amount of light passing through a solution unabsorbed or _________ is measured by ________ on the other side of the cuvette.

How do you prepare tissue for electron microscopy without using fixatives?

How do DNA molecules place themselves in a CsCl equilibrium gradient?

A(n)________ is a substance that reveals its presence in one way or another and thus can be localized or monitored during an experiment.

During the cloning procedure,what is the purpose of inserting genes into a bacterium that give it resistance to antibiotics?

What is the name of a piece of DNA containing sequences derived from more than one organism?

In which technique is radioactivity detected by mixing the sample in a vial with a special fluid that contains compounds that emit light if struck by a beta particle? The amount of light emitted in the fluid is a measure of the amount of radioactivity in sample.

What does cloning of cDNAs allow?

A plant cell that lacks a cell wall is called a(n)_________.

How are probes labeled with a radioactive isotope detected?

What is defined as viral-mediated gene transfer,which is a very widely used strategy to get genes into eukaryotic cells?

__________ are established with cells that have been obtained directly from the organism; most of these cultures produced from animal cells are obtained from ________.

Whole blood from an animal that has been exposed to foreign materials (antigens)and then had the cells and clotting factors removed from it is called _________.

Which part of a microscope is responsible for gathering diffuse rays from the microscope light source and illuminating the specimen with a small cone of bright light?

What does the change in field direction in pulsed field electrophoresis accomplish?

The magnification of a microscope is equal to __________.

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics,enzymes and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters

Antibodies made by a clone of antibody-producing cells derived from a single B lymphocyte have _; each different B cell has membrane-bound antibodies with an affinity for __.

The amount of light passing through a solution unabsorbed or _ is measured by on the other side of the cuvette.

__ are established with cells that have been obtained directly from the organism; most of these cultures produced from animal cells are obtained from .