Question 1

How does a phase-contract microscope setup make transparent objects more visible?

Accepted Answer

A) It uses brighter bulbs than conventional light microscopes 
B) It generates a negative image so that the background is dark and the specimen is light 
C) It generates stereo images that can be viewed only with polarized filters 
D) It causes direct and refracted light rays to interfere with one another 
E) It operates at a lower temperature to reduce random specimen movement 
A) It uses brighter bulbs than conventional light microscopes 
B) It generates a negative image so that the background is dark and the specimen is light 
C) It generates stereo images that can be viewed only with polarized filters 
D) It causes direct and refracted light rays to interfere with one another 
E) It operates at a lower temperature to reduce random specimen movement

Question 2

What happens if a photographic emulsion is brought into close contact with radioactive source in a specimen?

Accepted Answer

A) The emulsion is degraded in places close to a radioactive atom. 
B) The particles emitted by the source leave tiny,black silver grains in the emulsion after photographic development. 
C) The particles emitted by the source leave tiny,black potassium grains in the emulsion after photographic development. 
D) The particles emitted by the source leave tiny,silver carbon grains in the emulsion after photographic development. 
E) The particles emitted by the source leave tiny,gold atoms in the emulsion after photographic development. 
A) The emulsion is degraded in places close to a radioactive atom. 
B) The particles emitted by the source leave tiny,black silver grains in the emulsion after photographic development. 
C) The particles emitted by the source leave tiny,black potassium grains in the emulsion after photographic development. 
D) The particles emitted by the source leave tiny,silver carbon grains in the emulsion after photographic development. 
E) The particles emitted by the source leave tiny,gold atoms in the emulsion after photographic development.

Question 3

Nowadays,how are double-stranded molecules of DNA synthesized for use in experimental procedures?

Accepted Answer

A) by insertion into bacterial cells 
B) by insertion into bacteriophage 
C) by automated synthesis of single stranded DNA,followed by hybridization 
D) in quartz cuvettes 
E) by insertion into fungi 
A) by insertion into bacterial cells 
B) by insertion into bacteriophage 
C) by automated synthesis of single stranded DNA,followed by hybridization 
D) in quartz cuvettes 
E) by insertion into fungi

Question 4

Which of the following is a technique used to encourage bacteria to take up DNA from the surrounding culture medium?

Accepted Answer

A) pretreatment of bacteria with a brief heat shock,followed by exposure to Ca2+ ions 
B) pretreatment of bacteria with Cu2+ ions,followed by a brief heat shock 
C) pretreatment of bacteria with Ca2+ ions,followed by a brief heat shock 
D) pretreatment of bacteria with Ca2+ ions,followed by a brief cold shock 
E) pretreatment of bacteria with a brief heat shock,followed by exposure to Mg2+ ions 
A) pretreatment of bacteria with a brief heat shock,followed by exposure to Ca2+ ions 
B) pretreatment of bacteria with Cu2+ ions,followed by a brief heat shock 
C) pretreatment of bacteria with Ca2+ ions,followed by a brief heat shock 
D) pretreatment of bacteria with Ca2+ ions,followed by a brief cold shock 
E) pretreatment of bacteria with a brief heat shock,followed by exposure to Mg2+ ions

Question 5

In the cycle sequencing method,how is the sequence of the DNA determined?

Accepted Answer

A) from autoradiograms 
B) from the order of fluorescent colors in the capillary gel 
C) from the overall color of the gel 
D) from the amount of heavy nitrogen in each band on the gel 
E) from the absorbance read in the spectrophotometer 
A) from autoradiograms 
B) from the order of fluorescent colors in the capillary gel 
C) from the overall color of the gel 
D) from the amount of heavy nitrogen in each band on the gel 
E) from the absorbance read in the spectrophotometer

Question 6

How are double-stranded hybrids released from hydroxylapatite columns?

Accepted Answer

A) by increasing elution buffer concentration 
B) by decreasing elution buffer concentration 
C) by increasing pH 
D) by decreasing pH 
E) by increasing sucrose concentration 
A) by increasing elution buffer concentration 
B) by decreasing elution buffer concentration 
C) by increasing pH 
D) by decreasing pH 
E) by increasing sucrose concentration

Question 7

Which form of radiation is emitted by the most commonly used isotopes and by most biologically important isotopes?

Accepted Answer

A) an alpha particle 
B) a delta particle 
C) a beta particle 
D) gamma radiation 
E) an omega particle 
A) an alpha particle 
B) a delta particle 
C) a beta particle 
D) gamma radiation 
E) an omega particle

Question 8

Most proteins have an isoelectric point ________ 7.

Accepted Answer

A) equal to 
B) above 
C) below 
D) far above 
E) at or above 
A) equal to 
B) above 
C) below 
D) far above 
E) at or above

Question 9

Under what circumstances will a component in a solution or a suspension sediment through a centrifugal field?

Accepted Answer

A) if it has a greater density than the surrounding medium 
B) if it has a lower density than the surrounding medium 
C) if it is wet 
D) if the surrounding medium is denser than the component 
E) if the component and the surrounding medium have equal density 
A) if it has a greater density than the surrounding medium 
B) if it has a lower density than the surrounding medium 
C) if it is wet 
D) if the surrounding medium is denser than the component 
E) if the component and the surrounding medium have equal density

Question 10

A culture system in which cells are grown in a three-dimensional matrix consisting of synthetic and/or natural extracellular materials is called a ______.

Accepted Answer

A) three-dimensional culture system 
B) two-dimensional culture system 
C) matrix culture system 
D) secondary culture system 
E) organic culture system 
A) three-dimensional culture system 
B) two-dimensional culture system 
C) matrix culture system 
D) secondary culture system 
E) organic culture system

Question 11

In which chromatographic method are the charges of the proteins in question the basis of their purification?

Accepted Answer

A) affinity chromatography 
B) gel filtration 
C) HPLC 
D) ion exchange chromatography 
E) polyvalent chromatography 
A) affinity chromatography 
B) gel filtration 
C) HPLC 
D) ion exchange chromatography 
E) polyvalent chromatography

Question 12

The _______ of mammals is an early embryonic developmental stage comparable to the blastula stage in other animals.

Accepted Answer

A) blastoderm 
B) fetus 
C) blastocyst 
D) trophectoderm 
E) inner cell mass 
A) blastoderm 
B) fetus 
C) blastocyst 
D) trophectoderm 
E) inner cell mass

Question 13

The first fully human antibody has been manufactured using bacteriophage rather than hybridomas; it is approved for rheumatoid arthritis treatment and is called _________.

Accepted Answer

A) Phagoma 
B) Humira 
C) Humana 
D) Humantibody 
E) Amana 
A) Phagoma 
B) Humira 
C) Humana 
D) Humantibody 
E) Amana

Question 14

Which form of radiation is equivalent to a helium atom nucleus?

Accepted Answer

A) an alpha particle 
B) a delta particle 
C) a beta particle 
D) gamma radiation 
E) an omega particle 
A) an alpha particle 
B) a delta particle 
C) a beta particle 
D) gamma radiation 
E) an omega particle

Question 15

Which form of radiation is equivalent to an electron?

Accepted Answer

A) an alpha particle 
B) a delta particle 
C) a beta particle 
D) gamma radiation 
E) an omega particle 
A) an alpha particle 
B) a delta particle 
C) a beta particle 
D) gamma radiation 
E) an omega particle

Question 16

When a cell is growing on a culture dish,what is the word that refers to the lower surface of the cell facing toward the substratum?

Accepted Answer

A) anterior 
B) dorsal 
C) posterior 
D) ventral 
E) caudal 
A) anterior 
B) dorsal 
C) posterior 
D) ventral 
E) caudal

Question 17

What is the name of the process by which recombinant DNA gets into bacteria after being picked up from the surrounding medium?

Accepted Answer

A) transduction 
B) transmutation 
C) transformation 
D) transjunction 
E) transition 
A) transduction 
B) transmutation 
C) transformation 
D) transjunction 
E) transition

Question 18

If the photographic emulsion used in the autoradiography procedure is left in contact with the isotope for a longer period of time,what happens?

Accepted Answer

A) There are more exposed silver grains. 
B) There are fewer exposed silver grains. 
C) There is no change in the number of exposed silver grains. 
D) The entire specimen will be uniformly covered with exposed silver grains. 
E) There will be very few exposed silver grains. 
A) There are more exposed silver grains. 
B) There are fewer exposed silver grains. 
C) There is no change in the number of exposed silver grains. 
D) The entire specimen will be uniformly covered with exposed silver grains. 
E) There will be very few exposed silver grains.

Question 19

In what electrophoresis medium are small RNAs or DNAs of a few hundred nucleotides typically separated?

Accepted Answer

A) agarose 
B) polyacrylamide 
C) cellulose 
D) paper 
E) dextran 
A) agarose 
B) polyacrylamide 
C) cellulose 
D) paper 
E) dextran

Question 20

If you do site-directed mutagenesis of the coding region of a gene,which of the following might happen?

Accepted Answer

A) The rate of gene expression may slow down. 
B) The rate of gene expression may speed up. 
C) The rate of gene expression may remain the same. 
D) The protein's structure and function are both likely to be altered. 
E) The protein's structure and function are both extremely unlikely to be altered. 
A) The rate of gene expression may slow down. 
B) The rate of gene expression may speed up. 
C) The rate of gene expression may remain the same. 
D) The protein's structure and function are both likely to be altered. 
E) The protein's structure and function are both extremely unlikely to be altered.

How does a phase-contract microscope setup make transparent objects more visible?

What happens if a photographic emulsion is brought into close contact with radioactive source in a specimen?

Nowadays,how are double-stranded molecules of DNA synthesized for use in experimental procedures?

Which of the following is a technique used to encourage bacteria to take up DNA from the surrounding culture medium?

In the cycle sequencing method,how is the sequence of the DNA determined?

How are double-stranded hybrids released from hydroxylapatite columns?

Which form of radiation is emitted by the most commonly used isotopes and by most biologically important isotopes?

Most proteins have an isoelectric point ________ 7.

Under what circumstances will a component in a solution or a suspension sediment through a centrifugal field?

A culture system in which cells are grown in a three-dimensional matrix consisting of synthetic and/or natural extracellular materials is called a ______.

In which chromatographic method are the charges of the proteins in question the basis of their purification?

The _______ of mammals is an early embryonic developmental stage comparable to the blastula stage in other animals.

The first fully human antibody has been manufactured using bacteriophage rather than hybridomas; it is approved for rheumatoid arthritis treatment and is called _________.

Which form of radiation is equivalent to a helium atom nucleus?

Which form of radiation is equivalent to an electron?

When a cell is growing on a culture dish,what is the word that refers to the lower surface of the cell facing toward the substratum?

What is the name of the process by which recombinant DNA gets into bacteria after being picked up from the surrounding medium?

If the photographic emulsion used in the autoradiography procedure is left in contact with the isotope for a longer period of time,what happens?

In what electrophoresis medium are small RNAs or DNAs of a few hundred nucleotides typically separated?

If you do site-directed mutagenesis of the coding region of a gene,which of the following might happen?

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics,enzymes and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters

Exam 18: Techniques in Cell and Molecular Biology

How does a phase-contract microscope setup make transparent objects more visible?

What happens if a photographic emulsion is brought into close contact with radioactive source in a specimen?

Nowadays,how are double-stranded molecules of DNA synthesized for use in experimental procedures?

Which of the following is a technique used to encourage bacteria to take up DNA from the surrounding culture medium?

In the cycle sequencing method,how is the sequence of the DNA determined?

How are double-stranded hybrids released from hydroxylapatite columns?

Which form of radiation is emitted by the most commonly used isotopes and by most biologically important isotopes?

Most proteins have an isoelectric point ________ 7.

Under what circumstances will a component in a solution or a suspension sediment through a centrifugal field?

A culture system in which cells are grown in a three-dimensional matrix consisting of synthetic and/or natural extracellular materials is called a ______.

In which chromatographic method are the charges of the proteins in question the basis of their purification?

The _______ of mammals is an early embryonic developmental stage comparable to the blastula stage in other animals.

The first fully human antibody has been manufactured using bacteriophage rather than hybridomas; it is approved for rheumatoid arthritis treatment and is called _________.

Which form of radiation is equivalent to a helium atom nucleus?

Which form of radiation is equivalent to an electron?

When a cell is growing on a culture dish,what is the word that refers to the lower surface of the cell facing toward the substratum?

What is the name of the process by which recombinant DNA gets into bacteria after being picked up from the surrounding medium?

If the photographic emulsion used in the autoradiography procedure is left in contact with the isotope for a longer period of time,what happens?

In what electrophoresis medium are small RNAs or DNAs of a few hundred nucleotides typically separated?

If you do site-directed mutagenesis of the coding region of a gene,which of the following might happen?

Introduction to the Study of Cell and Molecular Biology

The Chemical Basis of Life

Bioenergetics,enzymes and Metabolism

The Structure and Function of the Plasma Membrane

Aerobic Respiration and the Mitochondrion

Photosynthesis and the Chloroplast

Interactions Between Cells and Their Environment

Cytoplasmic Membrane Systems: Structure, function, and Membrane Trafficking

The Cytoskeleton and Cell Motility

The Nature of the Gene and the Genome

Gene Expression: From Transcription to Translation

Control of Gene Expression

Dna Replication and Repair

Cellular Reproduction

Cell Signaling and Signal Transduction: Communication Between Cells

Cancer

The Immune Response

Filters