Question 1

Methylation of guanine to produce O6-methylguanine (mG)results in what change to DNA?

Accepted Answer

A) mG frequently pairs with thymine resulting in a GC $\to$ AT mutation 
B) mG loses its methoxy group resulting in hydrogen bonding with adenine and a bulge in the double helix 
C) mG is prone to deamination and eventually,depurination 
D) repair of mG adducts uses a radical mechanism that has the potential to further damage adjacent bases,particularly thymine 
E) none of the above 
A) mG frequently pairs with thymine resulting in a GC $\to$ AT mutation 
B) mG loses its methoxy group resulting in hydrogen bonding with adenine and a bulge in the double helix 
C) mG is prone to deamination and eventually,depurination 
D) repair of mG adducts uses a radical mechanism that has the potential to further damage adjacent bases,particularly thymine 
E) none of the above

Question 2

What role does integration host factor (IHF)play in site-specific recombination during phage $\lambda$ lysogeny?

Accepted Answer

A) binds host DNA forcing a 90S1U1P1$\circ$S1S1P0bend 
B) supercoils phage DNA 
C) cleaves host and phage DNA at specific sites to create sticky ends 
D) ligates host and phage DNA once recombination occurs 
E) all of the above 
A) binds host DNA forcing a 90S1U1P1$\circ$S1S1P0bend 
B) supercoils phage DNA 
C) cleaves host and phage DNA at specific sites to create sticky ends 
D) ligates host and phage DNA once recombination occurs 
E) all of the above

Question 3

Which of the following transposable elements is correctly defined?

Accepted Answer

A) insertion sequence: protein-encoding gene flanked by two insertion sequences 
B) composite transposon: protein-encoding gene + transposase and resolvase genes flanked by two insertion sequences 
C) class II transposon: protein-encoding gene + resolvase gene flanked by two insertion sequences 
D) class III transposon: results from infection of a bacteriophage such as Mu 
E) none of the above 
A) insertion sequence: protein-encoding gene flanked by two insertion sequences 
B) composite transposon: protein-encoding gene + transposase and resolvase genes flanked by two insertion sequences 
C) class II transposon: protein-encoding gene + resolvase gene flanked by two insertion sequences 
D) class III transposon: results from infection of a bacteriophage such as Mu 
E) none of the above

Question 4

In order for lysogeny to occur,the enzyme ______________ must insert the phage DNA into a specific site in the bacterial chromosome.

Accepted Answer

A) ligase 
B) lysogenase 
C) helicase 
D) integrase 
E) none of the above 
A) ligase 
B) lysogenase 
C) helicase 
D) integrase 
E) none of the above

Methylation of guanine to produce O⁶-methylguanine (mG)results in what change to DNA?

What role does integration host factor (IHF)play in site-specific recombination during phage $\lambda$ lysogeny?

Which of the following transposable elements is correctly defined?

In order for lysogeny to occur,the enzyme ______________ must insert the phage DNA into a specific site in the bacterial chromosome.

The Scope of Biochemistry

The Matrix of Life: Weak Interactions in an Aqueous Environment

The Energetics of Life

Nucleic Acids

Introduction to Proteins: the Primary Level of Protein Structure

The Three-Dimensional Structure of Proteins

Protein Function and Evolution

Contractile Proteins and Molecular Motors

Carbohydrates: Sugars,saccharides,glycans

Lipids,membranes and Cellular Transport

Enzymes: Biological Catalysts

Chemical Logic of Metabolism

Carbohydrate Metabolism: Glycolysis, gluconeogenesis, glycogen Metabolism, and the Pentose Phosphate Pathway

Citric Acid Cycle and Glyoxylate Cycle

Electron Transport, oxidative Phosphorylation, and Oxygen Metabolism

Photosynthesis

Lipid Metabolism I: Fatty Acids,triacylglycerols,and Lipoproteins

Interorgan and Intracellular Coordination of Energy Metabolism in Vertebrates

Lipid Metabolism Ii: Membrane Lipids, steroids, isoprenoids, and Eicosanoids

Metabolism of Nitrogenous Compounds I: Principles of Biosynthesis, utilization, and Turnover

Metabolism of Nitogenous Compounds Ii: Amino Acids, porphyrins, and Neurotransmitters

Nucleotide Metabolism

Mechanisms of Signal Transduction

Genes,genomes and Chromosomes

Dna Replication

Information Readout: Transcription and Post-Transcriptional Processing

Information Decoding: Translation and Post-Translational Protein Processing

Regulation of Gene Expression

Filters

Exam 26: Dna Restructuring: Repair,recombination,rearrangement,amplification

Methylation of guanine to produce O6-methylguanine (mG)results in what change to DNA?

What role does integration host factor (IHF)play in site-specific recombination during phage λ\lambdaλ lysogeny?

Which of the following transposable elements is correctly defined?

In order for lysogeny to occur,the enzyme ______________ must insert the phage DNA into a specific site in the bacterial chromosome.

The Scope of Biochemistry

The Matrix of Life: Weak Interactions in an Aqueous Environment

The Energetics of Life

Nucleic Acids

Introduction to Proteins: the Primary Level of Protein Structure

The Three-Dimensional Structure of Proteins

Protein Function and Evolution

Contractile Proteins and Molecular Motors

Carbohydrates: Sugars,saccharides,glycans

Lipids,membranes and Cellular Transport

Enzymes: Biological Catalysts

Chemical Logic of Metabolism

Carbohydrate Metabolism: Glycolysis, gluconeogenesis, glycogen Metabolism, and the Pentose Phosphate Pathway

Citric Acid Cycle and Glyoxylate Cycle

Electron Transport, oxidative Phosphorylation, and Oxygen Metabolism

Photosynthesis

Lipid Metabolism I: Fatty Acids,triacylglycerols,and Lipoproteins

Interorgan and Intracellular Coordination of Energy Metabolism in Vertebrates

Lipid Metabolism Ii: Membrane Lipids, steroids, isoprenoids, and Eicosanoids

Metabolism of Nitrogenous Compounds I: Principles of Biosynthesis, utilization, and Turnover

Metabolism of Nitogenous Compounds Ii: Amino Acids, porphyrins, and Neurotransmitters

Nucleotide Metabolism

Mechanisms of Signal Transduction

Genes,genomes and Chromosomes

Dna Replication

Information Readout: Transcription and Post-Transcriptional Processing

Information Decoding: Translation and Post-Translational Protein Processing

Regulation of Gene Expression

Filters

Methylation of guanine to produce O⁶-methylguanine (mG)results in what change to DNA?

What role does integration host factor (IHF)play in site-specific recombination during phage $\lambda$ lysogeny?