Question 1

For the polymerase chain reaction to occur,____.

Accepted Answer

A)  the reaction temperature must be cycled from low to high 
B)  all DNA fragments must be identical 
C)  the DNA must remain double stranded 
D)  a sticky end must be available for the ligase enzyme to function 
E)  a large sample of DNA is needed 
A)  the reaction temperature must be cycled from low to high 
B)  all DNA fragments must be identical 
C)  the DNA must remain double stranded 
D)  a sticky end must be available for the ligase enzyme to function 
E)  a large sample of DNA is needed A

Question 2

__________ can reveal the order of nucleotide bases in a fragment of DNA.

Accepted Answer

DNA sequencing

Question 3

Gel electrophoresis separates the DNA fragments according to ____.

Accepted Answer

A)  their stage in replication 
B)  their size 
C)  their ability to bind to the gel 
D)  the orientation of the nucleotides in the fragment 
E)  their type 
A)  their stage in replication 
B)  their size 
C)  their ability to bind to the gel 
D)  the orientation of the nucleotides in the fragment 
E)  their type B

Question 4

In step 2 (above)in the identification of the colony that contains a targeted gene,____.

Accepted Answer

A)  bacterial cells are cloned 
B)  bacterial colonies are transferred 
C)  bacterial cells are ruptured 
D)  DNA is lysed 
E)  cells with the targeted gene are identified 
A)  bacterial cells are cloned 
B)  bacterial colonies are transferred 
C)  bacterial cells are ruptured 
D)  DNA is lysed 
E)  cells with the targeted gene are identified

Question 5

__________ are multiple copies of a short DNA sequence that occur at a given locus.

Accepted Answer

Short tand

Question 6

The polymerase chain reaction (PCR)uses ________,nucleotides,and a heat-resistant DNA polymerase to rapidly increase the number of molecules of a DNA fragment.

Accepted Answer

The answer of The polymerase chain reaction (PCR)uses ________,nucleotides,and a...

Question 7

Which statement is true?

Accepted Answer

A)  There is no danger involved in recombinant DNA research in humans. 
B)  There is no danger involved in recombinant DNA research in bacteria. 
C)  There is no danger in releasing recombinant organisms into the environment. 
D)  Stringent safety rules make the use of recombinant DNA research possible. 
E)  Stringent safety rules make the use of recombinant DNA research impossible. 
A)  There is no danger involved in recombinant DNA research in humans. 
B)  There is no danger involved in recombinant DNA research in bacteria. 
C)  There is no danger in releasing recombinant organisms into the environment. 
D)  Stringent safety rules make the use of recombinant DNA research possible. 
E)  Stringent safety rules make the use of recombinant DNA research impossible.

Question 8

Recombinant DNA is any DNA that ____.

Accepted Answer

A)  has been cut by restriction enzymes 
B)  is at least four to eight base pairs in length 
C)  has had its gene sequence rearranged 
D)  consists of DNA from two or more organisms of the same or different species 
E)  inserted into plasmids 
A)  has been cut by restriction enzymes 
B)  is at least four to eight base pairs in length 
C)  has had its gene sequence rearranged 
D)  consists of DNA from two or more organisms of the same or different species 
E)  inserted into plasmids

Question 9

Small circular molecules of DNA in bacteria ____.

Accepted Answer

A)  are plasmids 
B)  are DNA fragments from their main chromosome 
C)  result from the activity of restriction enzymes 
D)  are eventually degraded 
E)  cannot survive outside of the cell 
A)  are plasmids 
B)  are DNA fragments from their main chromosome 
C)  result from the activity of restriction enzymes 
D)  are eventually degraded 
E)  cannot survive outside of the cell

Question 10

A collection of host cells that house cloned fragments representing expressed genes is a____.

Accepted Answer

A)  cDNA library. 
B)  transcribed library. 
C)  genomic library. 
D)  RNA library. 
E)  plasmid library. 
A)  cDNA library. 
B)  transcribed library. 
C)  genomic library. 
D)  RNA library. 
E)  plasmid library.

Question 11

The use of SNPs for analyzing genomes is based on ____.

Accepted Answer

A)  the nucleotide base pairing in the SNPs 
B)  the types of nucleotides in the SNPs 
C)  differences in SNPs among individuals 
D)  the ratio of purines to pyrimidines in SNPs 
E)  bonding patterns between nucleotides in the SNPs 
A)  the nucleotide base pairing in the SNPs 
B)  the types of nucleotides in the SNPs 
C)  differences in SNPs among individuals 
D)  the ratio of purines to pyrimidines in SNPs 
E)  bonding patterns between nucleotides in the SNPs

Question 12

RNA can be used as a template for the production of DNA through the action of ____.

Accepted Answer

A)  DNA polymerase 
B)  RNA polymerase 
C)  reverse transcriptase 
D)  ligase 
E)  restriction endonuclease 
A)  DNA polymerase 
B)  RNA polymerase 
C)  reverse transcriptase 
D)  ligase 
E)  restriction endonuclease

Question 13

Bacterial ____ may be used as cloning vectors.

Accepted Answer

A)  transcriptase 
B)  ligase 
C)  endonuclease 
D)  plasmids 
E)  polymerase 
A)  transcriptase 
B)  ligase 
C)  endonuclease 
D)  plasmids 
E)  polymerase

Question 14

________,a viral enzyme,is used to convert single-stranded RNA into ___________ for cloning.

Accepted Answer

Reverse tr

Question 15

One of the first successful applications of genetic engineering was the commercial production of ____.

Accepted Answer

A)  clotting factor 
B)  insulin 
C)  hemoglobin 
D)  collagen 
E)  human growth factor 
A)  clotting factor 
B)  insulin 
C)  hemoglobin 
D)  collagen 
E)  human growth factor

Question 16

Restriction enzymes cut double-stranded DNA _____.

Accepted Answer

A)  at noncoding areas between genes 
B)  at specific base sequences 
C)  between bacterial and viral DNAs 
D)  between purines and pyrimidines 
E)  between promoter and operator DNA sequences 
A)  at noncoding areas between genes 
B)  at specific base sequences 
C)  between bacterial and viral DNAs 
D)  between purines and pyrimidines 
E)  between promoter and operator DNA sequences

Question 17

To answer the following questions, refer to the five items listed below:
-The function of these is to cut apart foreign DNA molecules.

Accepted Answer

A) restriction enzymes 
B) recombinant DNA 
C) plasmids 
D) clones 
E) restriction sites 
A) restriction enzymes 
B) recombinant DNA 
C) plasmids 
D) clones 
E) restriction sites

Question 18

Which genes are probably being transferred from transgenic plants to non-transgenic ones via pollen carried by wind or insects?

Accepted Answer

A)  Insecticide resistance 
B)  Glyphosate resistance 
C)  Antibacterial resistance 
D)  Early ripening 
E) -carotene 
A)  Insecticide resistance 
B)  Glyphosate resistance 
C)  Antibacterial resistance 
D)  Early ripening 
E) -carotene

Question 19

The results of the treatment of 20 boys with SCID-X1 showed that ____.

Accepted Answer

A)  SCID-X1 can be cured through genetic engineering 
B)  none of those treated were cured of the disease 
C)  a bone marrow transplant can cure SCID-X1 
D)  our understanding of the human genome exceeds our ability to modify it 
E)  the "treatment" caused numerous unforeseen complications. 
A)  SCID-X1 can be cured through genetic engineering 
B)  none of those treated were cured of the disease 
C)  a bone marrow transplant can cure SCID-X1 
D)  our understanding of the human genome exceeds our ability to modify it 
E)  the "treatment" caused numerous unforeseen complications.

Question 20

Genetic engineering has produced which of the following animals?

Accepted Answer

A)  Wild caught salmon 
B)  Pigs 
C)  Dolly the sheep 
D)  Cows resistant to mad cow disease 
E)  Grass-fed beef 
A)  Wild caught salmon 
B)  Pigs 
C)  Dolly the sheep 
D)  Cows resistant to mad cow disease 
E)  Grass-fed beef

Exam 15: Studying and Manipulating Genomes

For the polymerase chain reaction to occur,____.

__________ can reveal the order of nucleotide bases in a fragment of DNA.

Gel electrophoresis separates the DNA fragments according to ____.

In step 2 (above)in the identification of the colony that contains a targeted gene,____.

__________ are multiple copies of a short DNA sequence that occur at a given locus.

The polymerase chain reaction (PCR)uses ________,nucleotides,and a heat-resistant DNA polymerase to rapidly increase the number of molecules of a DNA fragment.

Which statement is true?

Recombinant DNA is any DNA that ____.

Small circular molecules of DNA in bacteria ____.

A collection of host cells that house cloned fragments representing expressed genes is a____.

The use of SNPs for analyzing genomes is based on ____.

RNA can be used as a template for the production of DNA through the action of ____.

Bacterial ____ may be used as cloning vectors.

________,a viral enzyme,is used to convert single-stranded RNA into ___________ for cloning.

One of the first successful applications of genetic engineering was the commercial production of ____.

Restriction enzymes cut double-stranded DNA _____.

To answer the following questions, refer to the five items listed below: -The function of these is to cut apart foreign DNA molecules.

Which genes are probably being transferred from transgenic plants to non-transgenic ones via pollen carried by wind or insects?

The results of the treatment of 20 boys with SCID-X1 showed that ____.

Genetic engineering has produced which of the following animals?

Invitation to Biology

Life's Chemical Bases

Molecules of Life

Cell Structure

Ground Rules of Metabolism

Where it Starts—Photosynthesis

How Cells Release Chemical Energy

DNA Structure and Function

From DNA to Protein

Control of Gene Expression

How Cells Reproduce

Meiosis and Sexual Reproduction

Observing Patterns in Inherited Traits

Chromosomes and Human Inheritance

Evidence of Evolution

Processes of Evolution

Organizing Information about Species

Life's Origin and Early Evolution

Viruses, Bacteria, and Archaea

Protists: The Simplest Eukaryotes

The Land Plants

Fungi

Animal Evolution: The Invertebrates

Animal Evolution: The Chordates

Human Evolution

Plant Tissues

Plant Nutrition and Transport

Life Cycles of Flowering Plants

Communication Strategies in Plants

Animal Tissues and Organ Systems

Neural Control

Sensory Perception

Endocrine Control

Structural Support and Movement

Circulation

Immunity

Respiration

Digestion and Nutrition

Maintaining the Internal Environment

Animal Reproductive System

Animal Development

Animal Behavior

Population Ecology

Community Ecology

Ecosystems

The Biosphere

Human Impacts on the Biosphere

Filters

,a viral enzyme,is used to convert single-stranded RNA into ___ for cloning.