Question 1

In DNA synthesis, the _____________ strand is synthesized in a __________ fashion.

Accepted Answer

A) template; discontinuous 
B) template; continuous 
C) leading; discontinuous 
D) lagging; discontinuous 
E) none of the above 
A) template; discontinuous 
B) template; continuous 
C) leading; discontinuous 
D) lagging; discontinuous 
E) none of the above

Question 2

How does the gp32 protein facilitate both stabilization of single-stranded DNA as well as its renaturation to double-stranded DNA?

Accepted Answer

A) gp32 is an enzyme and thus its reaction of separating DNA strands is freely reversible 
B) gp32 binds only to regions of high A and T content so hydrogen bonding is reversible 
C) gp32 displays cooperativity in binding to single stranded DNA 
D) the gp32 C-terminal domain acts as a switch between weak and strong single stranded DNA binding 
E) none of the above 
A) gp32 is an enzyme and thus its reaction of separating DNA strands is freely reversible 
B) gp32 binds only to regions of high A and T content so hydrogen bonding is reversible 
C) gp32 displays cooperativity in binding to single stranded DNA 
D) the gp32 C-terminal domain acts as a switch between weak and strong single stranded DNA binding 
E) none of the above

Question 3

Which of the following is the best description for primase?

Accepted Answer

A) a DNA-dependent DNA polymerase 
B) an RNA-dependent DNA polymerase 
C) a DNA-dependent RNA polymerase 
D) an RNA-dependent RNA polymerase 
E) none of the above 
A) a DNA-dependent DNA polymerase 
B) an RNA-dependent DNA polymerase 
C) a DNA-dependent RNA polymerase 
D) an RNA-dependent RNA polymerase 
E) none of the above

Question 4

Which of the following is an 11-base consensus sequence that makes up the autonomously replicating sequence present in yeast DNA that is a requirement for the start of replication?

Accepted Answer

A) 5'-GTGCGTGCGTG-3' 
B) 5'-CCTCCTGGTGG-3' 
C) 5'-AAGCGCGCGCC-3' 
D) 5'-GGTGGACCTCC-3' 
E) 5'-TTTTATATTTT-3' 
A) 5'-GTGCGTGCGTG-3' 
B) 5'-CCTCCTGGTGG-3' 
C) 5'-AAGCGCGCGCC-3' 
D) 5'-GGTGGACCTCC-3' 
E) 5'-TTTTATATTTT-3'

Question 5

The fact that the following molecule serves as a substrate for DNA polymerase in place of dTTP proves what issue with regard to DNA replication?

Accepted Answer

A) DNA polymerase is highly error prone 
B) hydrogen bonding to N-3 of thymine is not critical for substrate recognition 
C) base stacking is the true driving force for nucleotide placement 
D) substrate shape might be as important as hydrogen bonding for DNA polymerase 
E) none of the above 
A) DNA polymerase is highly error prone 
B) hydrogen bonding to N-3 of thymine is not critical for substrate recognition 
C) base stacking is the true driving force for nucleotide placement 
D) substrate shape might be as important as hydrogen bonding for DNA polymerase 
E) none of the above

In DNA synthesis, the ___ strand is synthesized in a fashion.

How does the gp32 protein facilitate both stabilization of single-stranded DNA as well as its renaturation to double-stranded DNA?

Which of the following is the best description for primase?

Which of the following is an 11-base consensus sequence that makes up the autonomously replicating sequence present in yeast DNA that is a requirement for the start of replication?

The fact that the following molecule serves as a substrate for DNA polymerase in place of dTTP proves what issue with regard to DNA replication?

The Scope of Biochemistry

The Matrix of Life: Weak Interactions in an Aqueous Environment

The Energetics of Life

Nucleic Acids

Introduction to Proteins: the Primary Level of Protein Structure

The Three-Dimensional Structure of Proteins

Protein Function and Evolution

Contractile Proteins and Molecular Motors

Carbohydrates: Sugars, Saccharides, Glycans

Lipids, Membranes and Cellular Transport

Enzymes: Biological Catalysts

Chemical Logic of Metabolism

Carbohydrate Metabolism: Glycolysis, Gluconeogenesis, Glycogen Metabolism, and the Pentose Phosphate Pathway

Citric Acid Cycle and Glyoxylate Cycle

Electron Transport, Oxidative Phosphorylation, and Oxygen Metabolism

Photosynthesis

Lipid Metabolism I: Fatty Acids, Triacylglycerols, and Lipoproteins

Interorgan and Intracellular Coordination of Energy Metabolism in Vertebrates

Lipid Metabolism Ii: Membrane Lipids, Steroids, Isoprenoids, and Eicosanoids

Metabolism of Nitrogenous Compounds I: Principles of Biosynthesis, Utilization, and Turnover

Metabolism of Nitogenous Compounds II: Amino Acids, Porphyrins, and Neurotransmitters

Nucleotide Metabolism

Mechanisms of Signal Transduction

Genes, Genomes and Chromosomes

DNA Restructuring: Repair, Recombination, Rearrangement, Amplification

Information Readout: Transcription and Post-Transcriptional Processing

Information Decoding: Translation and Post-Translational Protein Processing

Regulation of Gene Expression

Filters

Exam 25: DNA Replication

In DNA synthesis, the _____________ strand is synthesized in a __________ fashion.

How does the gp32 protein facilitate both stabilization of single-stranded DNA as well as its renaturation to double-stranded DNA?

Which of the following is the best description for primase?

Which of the following is an 11-base consensus sequence that makes up the autonomously replicating sequence present in yeast DNA that is a requirement for the start of replication?

The fact that the following molecule serves as a substrate for DNA polymerase in place of dTTP proves what issue with regard to DNA replication?

The Scope of Biochemistry

The Matrix of Life: Weak Interactions in an Aqueous Environment

The Energetics of Life

Nucleic Acids

Introduction to Proteins: the Primary Level of Protein Structure

The Three-Dimensional Structure of Proteins

Protein Function and Evolution

Contractile Proteins and Molecular Motors

Carbohydrates: Sugars, Saccharides, Glycans

Lipids, Membranes and Cellular Transport

Enzymes: Biological Catalysts

Chemical Logic of Metabolism

Carbohydrate Metabolism: Glycolysis, Gluconeogenesis, Glycogen Metabolism, and the Pentose Phosphate Pathway

Citric Acid Cycle and Glyoxylate Cycle

Electron Transport, Oxidative Phosphorylation, and Oxygen Metabolism

Photosynthesis

Lipid Metabolism I: Fatty Acids, Triacylglycerols, and Lipoproteins

Interorgan and Intracellular Coordination of Energy Metabolism in Vertebrates

Lipid Metabolism Ii: Membrane Lipids, Steroids, Isoprenoids, and Eicosanoids

Metabolism of Nitrogenous Compounds I: Principles of Biosynthesis, Utilization, and Turnover

Metabolism of Nitogenous Compounds II: Amino Acids, Porphyrins, and Neurotransmitters

Nucleotide Metabolism

Mechanisms of Signal Transduction

Genes, Genomes and Chromosomes

DNA Restructuring: Repair, Recombination, Rearrangement, Amplification

Information Readout: Transcription and Post-Transcriptional Processing

Information Decoding: Translation and Post-Translational Protein Processing

Regulation of Gene Expression

Filters

In DNA synthesis, the ___ strand is synthesized in a fashion.