Question 1

One of the disadvantages of PCR is that it requires large amounts of starting material.

Accepted Answer

A) True 
 B)False

Question 2

Which of the following statements about PCR is not true?

Accepted Answer

A) PCR stands for "polymerizing copies of RNA."" 
B) PCR can create millions of copies of a segment of DNA in a test tube. 
C) PCR uses DNA polymerase. 
D) The amount of DNA doubles with each replication cycle. 
E) The target is repeatedly replicated. 
A) PCR stands for "polymerizing copies of RNA."" 
B) PCR can create millions of copies of a segment of DNA in a test tube. 
C) PCR uses DNA polymerase. 
D) The amount of DNA doubles with each replication cycle. 
E) The target is repeatedly replicated.

Question 3

Which of the following is a benefit gained from learning how to sequence DNA?

Accepted Answer

A) identification of faulty genes 
B) development of treatments for diseases 
C) modification of crops to increase yields 
D) understanding of our evolutionary history 
E) all of these are benefits gained from DNA sequencing 
A) identification of faulty genes 
B) development of treatments for diseases 
C) modification of crops to increase yields 
D) understanding of our evolutionary history 
E) all of these are benefits gained from DNA sequencing

Question 4

Which of the following is not true about transcription factors?

Accepted Answer

A) Transcription factors are DNA-binding proteins. 
B) Transcription factors are considered external cell regulators. 
C) There are many different types of transcription factors. 
D) Specialization of cells is determined/influenced by which transcription factors are active in the cell. 
E) Genes code for transcription factors. 
A) Transcription factors are DNA-binding proteins. 
B) Transcription factors are considered external cell regulators. 
C) There are many different types of transcription factors. 
D) Specialization of cells is determined/influenced by which transcription factors are active in the cell. 
E) Genes code for transcription factors.

Question 5

Which of the following is not a desirable trait for a genetically engineered plant?

Accepted Answer

A) disease resistant 
B) salt sensitive 
C) improved yield 
D) modified wood pulp 
E) drought tolerant 
A) disease resistant 
B) salt sensitive 
C) improved yield 
D) modified wood pulp 
E) drought tolerant

Question 6

Ex vivo gene therapy has been used to treat which of the following diseases?

Accepted Answer

A) SCID 
B) cardiovascular disease 
C) Huntington disease 
D) endocrine disorder 
E) all of these 
A) SCID 
B) cardiovascular disease 
C) Huntington disease 
D) endocrine disorder 
E) all of these

Question 7

Which of the following nucleotide sequences could give rise to the amino acid sequence alanine-asparatate-glycine? Assume that translation initiation has already occurred.

Accepted Answer

A) GUUAAGAGG 
B) GCCGACGGC 
C) CACCGCCGA 
D) GAUGGUGCU 
E) UUUUAAUGG 
A) GUUAAGAGG 
B) GCCGACGGC 
C) CACCGCCGA 
D) GAUGGUGCU 
E) UUUUAAUGG

Question 8

Place the following steps in order for the cloning of a gene.1.DNA ligase seals foreign DNA into a plasmid.2.A restriction enzyme is used to cleave DNA.3.The bacterium makes a product.4.Some of the bacterial cells take up a recombinant plasmid.5.The plasmid replicates on its own.

Accepted Answer

A) 2,1,4,5,3 
B) 1,2,3,4,5 
C) 3,1,2,4,5 
D) 5,1,4,3,2 
E) 1,3,2,4,5 
A) 2,1,4,5,3 
B) 1,2,3,4,5 
C) 3,1,2,4,5 
D) 5,1,4,3,2 
E) 1,3,2,4,5

Question 9

Which of the following is the primary goal of bioinformatics?

Accepted Answer

A) to find significant patterns in the information gained from DNA sequencing and proteomics 
B) to input DNA and protein sequences into a computer 
C) to model the three-dimensional shape of cellular proteins 
D) to obtain the sequence of all the noncoding regions of the DNA 
E) to insert genetic material into human cells for the treatment of a disorder 
A) to find significant patterns in the information gained from DNA sequencing and proteomics 
B) to input DNA and protein sequences into a computer 
C) to model the three-dimensional shape of cellular proteins 
D) to obtain the sequence of all the noncoding regions of the DNA 
E) to insert genetic material into human cells for the treatment of a disorder

Question 10

What is the name of the enzyme that fits new complementary DNA nucleotides into the new strand?

Accepted Answer

A) DNA polymerase 
B) DNA helicase 
C) DNA replicase 
D) DNA ligase 
E) DNA telomerase 
A) DNA polymerase 
B) DNA helicase 
C) DNA replicase 
D) DNA ligase 
E) DNA telomerase

Question 11

How much of your DNA is identical to the person who sits next to you in biology class?

Accepted Answer

A) 1% 
B) 50% 
C) 90% 
D) 99% 
E) 99.9% 
A) 1% 
B) 50% 
C) 90% 
D) 99% 
E) 99.9%

Question 12

Which of the following occurs during initiation of translation?

Accepted Answer

A) The ribosome moves down one codon so that the A site is available to receive an incoming tRNA. 
B) The mRNA binds to the smaller of the two ribosomal subunits. 
C) The polypeptide lengthens one amino acid at a time. 
D) The ribosome dissociates into its two subunits and falls off the mRNA. 
E) One of the three stop codons is reached. 
A) The ribosome moves down one codon so that the A site is available to receive an incoming tRNA. 
B) The mRNA binds to the smaller of the two ribosomal subunits. 
C) The polypeptide lengthens one amino acid at a time. 
D) The ribosome dissociates into its two subunits and falls off the mRNA. 
E) One of the three stop codons is reached.

Question 13

Which structure contains the anticodon?

Accepted Answer

A) the mRNA 
B) the large ribosomal subunit 
C) the small ribosomal subunit 
D) the tRNA 
E) the rRNA 
A) the mRNA 
B) the large ribosomal subunit 
C) the small ribosomal subunit 
D) the tRNA 
E) the rRNA

Question 14

How many different amino acids are commonly found in proteins?

Accepted Answer

A) 5 
B) 10 
C) 20 
D) 50 
E) over 100 
A) 5 
B) 10 
C) 20 
D) 50 
E) over 100

Question 15

Mitochondria also contain DNA.

Accepted Answer

A) True 
 B)False

Question 16

It only takes a matter of several hours to sequence 1000 base-pair sections of DNA.

Accepted Answer

A) True 
 B)False

Question 17

DNA replication is considered

Accepted Answer

A) conservative. 
B) semiconservative. 
C) dispersive. 
D) relaxeD. 
E) stringent. 
A) conservative. 
B) semiconservative. 
C) dispersive. 
D) relaxeD. 
E) stringent.

Question 18

How is the recombinant product procured from transgenic bacteria? Transgenic goats?

Accepted Answer

A) eat them,from growth media 
B) from growth media,drink milk 
C) eat them,eat them 
D) drink milk,drink milk 
E) from growth media,from growth media 
A) eat them,from growth media 
B) from growth media,drink milk 
C) eat them,eat them 
D) drink milk,drink milk 
E) from growth media,from growth media

Question 19

In vivo gene therapy has been used to help patients suffering from cystic fibrosis.

Accepted Answer

A) True 
 B)False

Question 20

Both DNA and RNA are

Accepted Answer

A) found in the cytoplasm. 
B) transcribed. 
C) translated. 
D) found in the nucleus. 
E) considered the genetic material of the cell. 
A) found in the cytoplasm. 
B) transcribed. 
C) translated. 
D) found in the nucleus. 
E) considered the genetic material of the cell.

Exam 22: DNA Biology and Technology

One of the disadvantages of PCR is that it requires large amounts of starting material.

Which of the following statements about PCR is not true?

Which of the following is a benefit gained from learning how to sequence DNA?

Which of the following is not true about transcription factors?

Which of the following is not a desirable trait for a genetically engineered plant?

Ex vivo gene therapy has been used to treat which of the following diseases?

Which of the following nucleotide sequences could give rise to the amino acid sequence alanine-asparatate-glycine? Assume that translation initiation has already occurred.

Place the following steps in order for the cloning of a gene.1.DNA ligase seals foreign DNA into a plasmid.2.A restriction enzyme is used to cleave DNA.3.The bacterium makes a product.4.Some of the bacterial cells take up a recombinant plasmid.5.The plasmid replicates on its own.

Which of the following is the primary goal of bioinformatics?

What is the name of the enzyme that fits new complementary DNA nucleotides into the new strand?

How much of your DNA is identical to the person who sits next to you in biology class?

Which of the following occurs during initiation of translation?

Which structure contains the anticodon?

How many different amino acids are commonly found in proteins?

Mitochondria also contain DNA.

It only takes a matter of several hours to sequence 1000 base-pair sections of DNA.

DNA replication is considered

How is the recombinant product procured from transgenic bacteria? Transgenic goats?

In vivo gene therapy has been used to help patients suffering from cystic fibrosis.

Both DNA and RNA are

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