Exam 13: An Introduction to Genetic Technology

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Edwin Southern and his colleagues started the field of genomics.

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In the mid-1970s a group of scientists asked the National Academy of Sciences to appoint a panel to assess the risks of recombinant DNA technology and the need to control its associated research.Summarize the most important lesson learned from this event.

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Plant and animal food sources have been improved by ____________________ for thousands of years.

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Most often,genomic library screening uses ____.

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A collection of clones containing all the DNA sequences carried by an individual is called a(n)____________________.

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The human genome contains approximately 40,000 genes.

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In a typical application,a DNA microarray is used to compare the ____________________.

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Plasmids are short sequences of DNA used as primers in PCR.

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A restriction enzyme is a ____.

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As the first steps in creating recombinant DNA molecules were taking place,scientists immediately realized that modifying the genetic information in ____________________,a bacterium from the human gut,could be potentially dangerous.

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During PCR,____________________ takes place when primers for DNA replication bind to complementary regions on the single-stranded DNA fragments.

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Explain why identical twins are clones.

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Discuss the benefits of making genome sequencing techniques a routine part of medical care.

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A DNA microarray is ____.

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Summarize the major advantage of using YACs to carry genome libraries.

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Repeating the sequence of the polymerase chain reaction doubles the number of DNA fragments each time.

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PCR uses two components: ____________________ that serve as templates and primers that bind to regions on either side of the template to be copied.

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Describe a benefit of cloning plants and animals.

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During the Southern blot technique,the cut DNA in the gel is ____________________ to single strands to enable a labeled probe to bind to complementary sequences of interest in the fragments.

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A human ____________________ can be carried in just over 3,000 YACs.

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