Question 1

Components of the spliceosome include

Accepted Answer

A) a pre-mRNA 
B) proteins that react immunologically with the sera of patients with systemic lupus erythematosus. 
C) U1 snRNA,which interacts with the 5´ splice site in pre-mRNA. 
D) all of the above 
A) a pre-mRNA 
B) proteins that react immunologically with the sera of patients with systemic lupus erythematosus. 
C) U1 snRNA,which interacts with the 5´ splice site in pre-mRNA. 
D) all of the above

Question 2

Which type of RNA participates in nuclear export of mRNA?

Accepted Answer

A) snRNA 
B) hnRNA 
C) tRNA 
D) rRNA 
A) snRNA 
B) hnRNA 
C) tRNA 
D) rRNA

Question 3

What are the main features of splicing pre-tRNAs that distinguish it from splicing pre-mRNAs?

Accepted Answer

Splicing of pre-tRNA does not involve sp

Question 4

How do researchers visualize the cellular locations of specific RNA molecules?

Accepted Answer

In one method,researchers manipulate the

Question 5

The RNA recognition motif is the most common RNA-binding domain in hnRNP proteins.Another name for this motif is:

Accepted Answer

A) RNA-binding domain. 
B) RNA motif. 
C) KH motif. 
D) all of the above 
A) RNA-binding domain. 
B) RNA motif. 
C) KH motif. 
D) all of the above

Question 6

Describe how the Sex-lethal (Sxl)protein is regulated during the development of Drosophila females.

Accepted Answer

Early in development,females utilize the

Question 7

In yeast,U2 snRNA base-pairs to a short sequence near branch-point A in introns.In higher eukaryotes,this branch-point sequence is not highly conserved,and a protein called U2AF promotes binding of U2 snRNA to pre-mRNA.You have produced mice with a knockout mutation in the U2AF gene.Would you expect mice heterozygous for the U2AF knockout mutation to be viable? Would you expect mice homozygous for the U2AF knockout mutation to be viable?

Accepted Answer

Association of the U2 snRNP with pre-mRN

Question 8

pre-mRNPs that are capped,spliced,and cleaved must be _____ before they are called nuclear mRNPs.

Accepted Answer

A) elongated 
B) phosphorylated 
C) polyadenylated 
D) transcribed 
A) elongated 
B) phosphorylated 
C) polyadenylated 
D) transcribed

Question 9

RNA editing is

Accepted Answer

A) post-transcriptional alteration of mRNA sequences. 
B) pretranscriptional alteration of RNA sequences. 
C) post-transcriptional joining of two RNA molecules. 
D) none of the above 
A) post-transcriptional alteration of mRNA sequences. 
B) pretranscriptional alteration of RNA sequences. 
C) post-transcriptional joining of two RNA molecules. 
D) none of the above

Question 10

How does HIV bypass the normal restriction that prevents unspliced mRNAs from being transported from the nucleus to the cytoplasm?

Accepted Answer

The HIV genome codes for a viral protein

Question 11

In an experiment you have used recombinant DNA technology to create hnRNP C protein tagged with green fluorescent protein and hnRNP A1 protein tagged with red fluorescent protein.What would you expect to see when the proteins are expressed and visualized in Xenopus cells?

Accepted Answer

A) green fluorescence only in the cytoplasm 
B) red fluorescence only in the nucleus 
C) red fluorescence only in the cytoplasm 
D) green and red fluorescence in the nucleus 
A) green fluorescence only in the cytoplasm 
B) red fluorescence only in the nucleus 
C) red fluorescence only in the cytoplasm 
D) green and red fluorescence in the nucleus

Question 12

A ribozyme is an RNA sequence

Accepted Answer

A) that uses Mg²⁺ ions as a cofactor. 
B) with catalytic ability to cleave RNA. 
C) that acts in the spliceosome. 
D) all of the above 
A) that uses Mg²⁺ ions as a cofactor. 
B) with catalytic ability to cleave RNA. 
C) that acts in the spliceosome. 
D) all of the above

Question 13

Splice sites in pre-mRNA are marked by two universally conserved sequences located

Accepted Answer

A) in the middle of introns. 
B) at the ends of exons. 
C) at the ends of introns. 
D) none of the above 
A) in the middle of introns. 
B) at the ends of exons. 
C) at the ends of introns. 
D) none of the above

Question 14

Which of the following does not take part in the degradation process of eukaryotic mRNAs?

Accepted Answer

A) capping 
B) endonucleolytic cleavage 
C) exonucleolytic decay 
D) poly(
A) shortening 
A) capping 
B) endonucleolytic cleavage 
C) exonucleolytic decay 
D) poly(
A) shortening

Question 15

The 45S pre-rRNA molecule

Accepted Answer

A) can organize a nucleolus when present in a single copy. 
B) is encoded by tandemly arranged genes. 
C) is methylated on specific bases. 
D) all of the above 
A) can organize a nucleolus when present in a single copy. 
B) is encoded by tandemly arranged genes. 
C) is methylated on specific bases. 
D) all of the above

Question 16

In animal cells,nearly all cytoplasmic mRNAs have a 3´ poly(A)tail,which is added to the pre-mRNA before splicing.What proteins are involved in polyadenylation? Indicate their order of association with pre-mRNA and their functions.

Accepted Answer

(1)Poly(A)signal,which often is an AAUAA

Question 17

This type of RNA functions in the removal of introns from pre-RNAs.

Accepted Answer

A) snRNA (small nuclear RN
A)  
B) snoRNA (small nucleolar RN
A)  
C) siRNA (small interfering RN
A)  
D) miRNA (micro RN
A)  
A) snRNA (small nuclear RN
A)  
B) snoRNA (small nucleolar RN
A)  
C) siRNA (small interfering RN
A)  
D) miRNA (micro RN
A)

Question 18

snRNP-dependent splicing of pre-mRNA is thought to have evolved from the self-splicing properties inherent in the sequence of either group I or II introns.Alternative splicing of pre-mRNAs processed by spliceosomes has been demonstrated,whereas this phenomenon does not occur in RNA transcripts that undergo self-splicing.Explain this difference.

Accepted Answer

In the case where splicing is self-media

Question 19

Cells use several ways to avoid the translation of improperly processed mRNA molecules._____ is(are)considered mRNA surveillance mechanisms.

Accepted Answer

A) Nonsense-mediated decay 
B) Non-stop decay 
C) No-go decay 
D) all of the above 
A) Nonsense-mediated decay 
B) Non-stop decay 
C) No-go decay 
D) all of the above

Question 20

Transport of unspliced HIV mRNA from the nucleus to the cytoplasm of host cells is promoted by a virus-encoded protein named

Accepted Answer

A) Tat. 
B) Rev. 
C) nucleoplasmin. 
D) Ran. 
A) Tat. 
B) Rev. 
C) nucleoplasmin. 
D) Ran.

Exam 9: Post-Transcriptional Gene Control

Components of the spliceosome include

Which type of RNA participates in nuclear export of mRNA?

What are the main features of splicing pre-tRNAs that distinguish it from splicing pre-mRNAs?

How do researchers visualize the cellular locations of specific RNA molecules?

The RNA recognition motif is the most common RNA-binding domain in hnRNP proteins.Another name for this motif is:

Describe how the Sex-lethal (Sxl)protein is regulated during the development of Drosophila females.

pre-mRNPs that are capped,spliced,and cleaved must be _____ before they are called nuclear mRNPs.

RNA editing is

How does HIV bypass the normal restriction that prevents unspliced mRNAs from being transported from the nucleus to the cytoplasm?

In an experiment you have used recombinant DNA technology to create hnRNP C protein tagged with green fluorescent protein and hnRNP A1 protein tagged with red fluorescent protein.What would you expect to see when the proteins are expressed and visualized in Xenopus cells?

A ribozyme is an RNA sequence

Splice sites in pre-mRNA are marked by two universally conserved sequences located

Which of the following does not take part in the degradation process of eukaryotic mRNAs?

The 45S pre-rRNA molecule

In animal cells,nearly all cytoplasmic mRNAs have a 3´ poly(A)tail,which is added to the pre-mRNA before splicing.What proteins are involved in polyadenylation? Indicate their order of association with pre-mRNA and their functions.

This type of RNA functions in the removal of introns from pre-RNAs.

Cells use several ways to avoid the translation of improperly processed mRNA molecules._____ is(are)considered mRNA surveillance mechanisms.

Transport of unspliced HIV mRNA from the nucleus to the cytoplasm of host cells is promoted by a virus-encoded protein named

Chemical Foundations

Molecular Genetic Techniques

Protein Structure and Function

Culturing and Visualizing Cells

Fundamental Molecular Genetic Mechanisms

Bio-membrane Structure

Genes Genomics and Chromosomes

Transcriptional Control of Gene Expression

Transmembrane Transport of Ions and Small Molecules

Cellular Energetics

Moving Proteins Into Membranes and Organelles

Vesicular Traffic, Secretion, and Endocytosis

Signal Transduction and G-Protein Coupled Receptors

Signaling Pathways That Control Gene Activity

Cell Organization and Movement I: Microfilaments

Cell Organization and Movement II: Microtubules and Intermediate Filaments

Regulating the Eukaryotic Cell Cycle

Integrating Cells Into Tissues

Cell Birth, Lineage, and Death

Nerve Cells

Immunology

Cancer

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