Question 1

The TATA box

Accepted Answer

A) serves as a promoter sequence for genes transcribed by RNA polymerase III. 
B) is located approximately 100 base pairs upstream of the start site for mRNAs. 
C) is present in all eukaryotic genes. 
D) acts to position RNA polymerase II for transcription initiation. 
A) serves as a promoter sequence for genes transcribed by RNA polymerase III. 
B) is located approximately 100 base pairs upstream of the start site for mRNAs. 
C) is present in all eukaryotic genes. 
D) acts to position RNA polymerase II for transcription initiation.

Question 2

What is the function of TFIIH in the transcription initiation complex?

Accepted Answer

A) binding to the TATA box 
B) unwinding the DNA duplex 
C) catalyzing the synthesis of RNA 
D) all of the above 
A) binding to the TATA box 
B) unwinding the DNA duplex 
C) catalyzing the synthesis of RNA 
D) all of the above

Question 3

Which of the following is not a structural motif found in a DNA-binding domain?

Accepted Answer

A) homeodomain 
B) zinc-finger 
C) helix-loop-helix 
D) random-coil acidic domain 
A) homeodomain 
B) zinc-finger 
C) helix-loop-helix 
D) random-coil acidic domain

Question 4

Define the terms cis-acting DNA sequences and trans-acting proteins.

Accepted Answer

Cis-acting DNA elements affect only the

Question 5

What family of proteins plays an essential role in the repression of genes that help to direct the formation of specific tissues and organs in a developing embryo?

Accepted Answer

A) Retinoblastoma 
B) Trithorax 
C) Polycomb 
D) Pax 
A) Retinoblastoma 
B) Trithorax 
C) Polycomb 
D) Pax

Question 6

How can transcription factors be purified using sequence-specific DNA-affinity chromatography?

Accepted Answer

Sequence-specific DNA-affinity chromatog

Question 7

The nuclear-receptor superfamily consists of several proteins that bind to consensus sequences of DNA response elements.Which of the following is NOT considered a member of this superfamily?

Accepted Answer

A) retinoic acid receptor 
B) acetylcholine receptor 
C) glucocorticoid receptor 
D) progesterone receptor 
A) retinoic acid receptor 
B) acetylcholine receptor 
C) glucocorticoid receptor 
D) progesterone receptor

Question 8

Sigma factors present in bacteria are proteins required to:

Accepted Answer

A) allow translation to proceed. 
B) terminate DNA replication. 
C) initiate transcription. 
D) terminate DNA replication and initiate transcription. 
A) allow translation to proceed. 
B) terminate DNA replication. 
C) initiate transcription. 
D) terminate DNA replication and initiate transcription.

Question 9

An enhancer

Accepted Answer

A) is a DNA element that stimulates transcription of eukaryotic promoters. 
B) binds to RNA polymerase and stimulates transcription. 
C) acts as a binding site for RNA polymerase. 
D) interacts with repressor proteins to enhance transcriptional repression. 
A) is a DNA element that stimulates transcription of eukaryotic promoters. 
B) binds to RNA polymerase and stimulates transcription. 
C) acts as a binding site for RNA polymerase. 
D) interacts with repressor proteins to enhance transcriptional repression.

Question 10

You are studying the expression of the gene that appears to be under the control of three different transcription-control regions during mouse embryonic development.Which one of the following is the BEST method to use to determine when each of these regions are active in the developing mouse embryo?

Accepted Answer

A) DNA affinity chromatography 
B) polymerase chain reaction 
C) reporter gene assay 
D) DNAse 1 footprinting 
A) DNA affinity chromatography 
B) polymerase chain reaction 
C) reporter gene assay 
D) DNAse 1 footprinting

Question 11

Describe the mechanism of transcriptional control for the heat shock genes.What advantage does this type of control impart to the cell?

Accepted Answer

During transcription of the heat-shock g

The TATA box

What is the function of TFIIH in the transcription initiation complex?

Which of the following is not a structural motif found in a DNA-binding domain?

Define the terms cis-acting DNA sequences and trans-acting proteins.

What family of proteins plays an essential role in the repression of genes that help to direct the formation of specific tissues and organs in a developing embryo?

How can transcription factors be purified using sequence-specific DNA-affinity chromatography?

The nuclear-receptor superfamily consists of several proteins that bind to consensus sequences of DNA response elements.Which of the following is NOT considered a member of this superfamily?

Sigma factors present in bacteria are proteins required to:

An enhancer

You are studying the expression of the gene that appears to be under the control of three different transcription-control regions during mouse embryonic development.Which one of the following is the BEST method to use to determine when each of these regions are active in the developing mouse embryo?

Describe the mechanism of transcriptional control for the heat shock genes.What advantage does this type of control impart to the cell?

Chemical Foundations

Molecular Genetic Techniques

Protein Structure and Function

Culturing and Visualizing Cells

Fundamental Molecular Genetic Mechanisms

Bio-membrane Structure

Genes Genomics and Chromosomes

Post-Transcriptional Gene Control

Transmembrane Transport of Ions and Small Molecules

Cellular Energetics

Moving Proteins Into Membranes and Organelles

Vesicular Traffic, Secretion, and Endocytosis

Signal Transduction and G-Protein Coupled Receptors

Signaling Pathways That Control Gene Activity

Cell Organization and Movement I: Microfilaments

Cell Organization and Movement II: Microtubules and Intermediate Filaments

Regulating the Eukaryotic Cell Cycle

Integrating Cells Into Tissues

Cell Birth, Lineage, and Death

Nerve Cells

Immunology

Cancer

Filters

Exam 8: Transcriptional Control of Gene Expression

The TATA box

What is the function of TFIIH in the transcription initiation complex?

Which of the following is not a structural motif found in a DNA-binding domain?

Define the terms cis-acting DNA sequences and trans-acting proteins.

What family of proteins plays an essential role in the repression of genes that help to direct the formation of specific tissues and organs in a developing embryo?

How can transcription factors be purified using sequence-specific DNA-affinity chromatography?

The nuclear-receptor superfamily consists of several proteins that bind to consensus sequences of DNA response elements.Which of the following is NOT considered a member of this superfamily?

Sigma factors present in bacteria are proteins required to:

An enhancer

Describe the mechanism of transcriptional control for the heat shock genes.What advantage does this type of control impart to the cell?

Chemical Foundations

Molecular Genetic Techniques

Protein Structure and Function

Culturing and Visualizing Cells

Fundamental Molecular Genetic Mechanisms

Bio-membrane Structure

Genes Genomics and Chromosomes

Post-Transcriptional Gene Control

Transmembrane Transport of Ions and Small Molecules

Cellular Energetics

Moving Proteins Into Membranes and Organelles

Vesicular Traffic, Secretion, and Endocytosis

Signal Transduction and G-Protein Coupled Receptors

Signaling Pathways That Control Gene Activity

Cell Organization and Movement I: Microfilaments

Cell Organization and Movement II: Microtubules and Intermediate Filaments

Regulating the Eukaryotic Cell Cycle

Integrating Cells Into Tissues

Cell Birth, Lineage, and Death

Nerve Cells

Immunology

Cancer

Filters