Question 1

Lipid soluble hormones activate transcription by

Accepted Answer

A) binding to specific cell-surface receptors. 
B) phosphorylating a protein kinase. 
C) binding to a nuclear receptor. 
D) inhibiting a histone deacetylase. 
A) binding to specific cell-surface receptors. 
B) phosphorylating a protein kinase. 
C) binding to a nuclear receptor. 
D) inhibiting a histone deacetylase.

Question 2

All the following elements can function as eukaryotic promoters except

Accepted Answer

A) a TATA box. 
B) an initiator element. 
C) CpG islands. 
D) an enhancer. 
A) a TATA box. 
B) an initiator element. 
C) CpG islands. 
D) an enhancer.

Question 3

You have identified a transcription factor and hypothesize that it binds to the promoter region of a gene that encodes a protein that causes cells to stop dividing.In order to test the interaction between the transcription factor and the DNA you will need to do a specific assay.Which one of the following would you use to test your hypothesis?

Accepted Answer

A) fluorescent in situ hybridization 
B) chromatin immunoprecipitation 
C) immunocytochemistry 
D) high-throughput DNA sequencing 
A) fluorescent in situ hybridization 
B) chromatin immunoprecipitation 
C) immunocytochemistry 
D) high-throughput DNA sequencing

Question 4

You are studying the effects of α-amanitin,a poisonous cyclic octapeptide,on eukaryotic cells and have noticed that following treatment,there is no miRNA transcription.Based on this evidence you conclude that α-amanitin must be inhibiting:

Accepted Answer

A) RNA polymerase I. 
B) RNA polymerase II. 
C) RNA polymerase III. 
D) RNA polymerase I and RNA polymerase III. 
A) RNA polymerase I. 
B) RNA polymerase II. 
C) RNA polymerase III. 
D) RNA polymerase I and RNA polymerase III.

Question 5

All of the following statements about the essential carboxy terminal domain (CTD)of RNA polymerase are true except:

Accepted Answer

A) The CTD is present in RNA polymerase I,II,and III. 
B) The CTD can become phosphorylated. 
C) The CTD is critical for viability. 
D) The CTD of mammals contains more than 50 repeats of a heptapeptide. 
A) The CTD is present in RNA polymerase I,II,and III. 
B) The CTD can become phosphorylated. 
C) The CTD is critical for viability. 
D) The CTD of mammals contains more than 50 repeats of a heptapeptide.

Question 6

Reporter genes employ fragments of DNA encoding proteins that when translated do not have any obvious effects in the cells and tissues.Which one of the following is NOT a reporter protein?

Accepted Answer

A) luciferase 
B) green fluorescent protein 
C)  β-galactosidase 
D) RNA polymerase 1 
A) luciferase 
B) green fluorescent protein 
C)  β-galactosidase 
D) RNA polymerase 1

Question 7

Describe the structure of the RNA polymerase II transcription initiation complex.

Accepted Answer

The RNA polymerase II transcription init

Question 8

Bromodomains are found in chromosome-associated proteins that contribute to transcriptional activation.To facilitate this activation,the bromodomains bind to histones,specifically their lysine residues that have been post-translationally modified by:

Accepted Answer

A) methylation. 
B) acetylation. 
C) phosphorylation. 
D) ubiquitination. 
A) methylation. 
B) acetylation. 
C) phosphorylation. 
D) ubiquitination.

Question 9

X chromosome inactivation in mammals is mediated by

Accepted Answer

A) micro RNAs (miRN
A) . 
B) long non-coding RNAs (ncRN
A) . 
C) messenger RNA (mRN
A) . 
D) short RNA-directed methylation of histones and DNA. 
A) micro RNAs (miRN
A) . 
B) long non-coding RNAs (ncRN
A) . 
C) messenger RNA (mRN
A) . 
D) short RNA-directed methylation of histones and DNA.

Question 10

Which of the following is the correct order of binding of general transcription factors to initiate transcription at RNA polymerase II promoters?

Accepted Answer

A) TFIID,TFIIB,Pol II,TFIIH 
B) PolII,TFIID,TFIIB,TFIIH 
C) TFIIB,PolII,TFIIH,TFIID 
D) TFIID,TFIIH,TFIIB,PolII 
A) TFIID,TFIIB,Pol II,TFIIH 
B) PolII,TFIID,TFIIB,TFIIH 
C) TFIIB,PolII,TFIIH,TFIID 
D) TFIID,TFIIH,TFIIB,PolII

Question 11

Describe the similarities and differences between prokaryotic and eukaryotic RNA polymerases.

Accepted Answer

In prokaryotes,there is only one RNA pol

Question 12

What is the functional difference between enhancers and promoter proximal elements?

Accepted Answer

Enhancers can stimulate transcription fr

Question 13

Which of the following proteins does not "footprint" the lac operon control region?

Accepted Answer

A) lac repressor 
B) β-galactosidase 
C) RNA polymerase 
D) cAMP-CAP 
A) lac repressor 
B) β-galactosidase 
C) RNA polymerase 
D) cAMP-CAP

Question 14

The mediator complex

Accepted Answer

A) can form a molecular bridge between activators of transcription and DNA replication machinery. 
B) can function to maintain a promoter in a hypoacetylated state. 
C) has histone acetylase activity. 
D) none of the above 
A) can form a molecular bridge between activators of transcription and DNA replication machinery. 
B) can function to maintain a promoter in a hypoacetylated state. 
C) has histone acetylase activity. 
D) none of the above

Question 15

Describe how lipid soluble hormones,glucocorticoid for example,regulate gene transcription acting through nuclear hormone receptors.

Accepted Answer

Glucocorticoid is a lipid soluble hormon

Question 16

What is an enhanceosome?

Accepted Answer

An enhanceosome is a large nucleoprotein

Question 17

Transcriptionally inactive genes

Accepted Answer

A) are always located within euchromatin. 
B) are not located within nucleosomes. 
C) often are methylated. 
D) are not resistant to DNase I. 
A) are always located within euchromatin. 
B) are not located within nucleosomes. 
C) often are methylated. 
D) are not resistant to DNase I.

Question 18

Describe the structure and function of a zinc-finger motif.

Accepted Answer

A zinc finger is a structural motif foun

Question 19

Describe the functional properties of TFIID and TFIIH.

Accepted Answer

TFIID is a large,multisubunit complex of

Question 20

Describe the structure and function of the carboxy terminal domain (CTD)of RNA polymerase II.

Accepted Answer

The carboxy terminal domain (CTD)of RNA

Exam 8: Transcriptional Control of Gene Expression

Lipid soluble hormones activate transcription by

All the following elements can function as eukaryotic promoters except

You are studying the effects of α-amanitin,a poisonous cyclic octapeptide,on eukaryotic cells and have noticed that following treatment,there is no miRNA transcription.Based on this evidence you conclude that α-amanitin must be inhibiting:

All of the following statements about the essential carboxy terminal domain (CTD)of RNA polymerase are true except:

Reporter genes employ fragments of DNA encoding proteins that when translated do not have any obvious effects in the cells and tissues.Which one of the following is NOT a reporter protein?

Describe the structure of the RNA polymerase II transcription initiation complex.

Bromodomains are found in chromosome-associated proteins that contribute to transcriptional activation.To facilitate this activation,the bromodomains bind to histones,specifically their lysine residues that have been post-translationally modified by:

X chromosome inactivation in mammals is mediated by

Which of the following is the correct order of binding of general transcription factors to initiate transcription at RNA polymerase II promoters?

Describe the similarities and differences between prokaryotic and eukaryotic RNA polymerases.

What is the functional difference between enhancers and promoter proximal elements?

Which of the following proteins does not "footprint" the lac operon control region?

The mediator complex

Describe how lipid soluble hormones,glucocorticoid for example,regulate gene transcription acting through nuclear hormone receptors.

What is an enhanceosome?

Transcriptionally inactive genes

Describe the structure and function of a zinc-finger motif.

Describe the functional properties of TFIID and TFIIH.

Describe the structure and function of the carboxy terminal domain (CTD)of RNA polymerase II.

Chemical Foundations

Molecular Genetic Techniques

Protein Structure and Function

Culturing and Visualizing Cells

Fundamental Molecular Genetic Mechanisms

Bio-membrane Structure

Genes Genomics and Chromosomes

Post-Transcriptional Gene Control

Transmembrane Transport of Ions and Small Molecules

Cellular Energetics

Moving Proteins Into Membranes and Organelles

Vesicular Traffic, Secretion, and Endocytosis

Signal Transduction and G-Protein Coupled Receptors

Signaling Pathways That Control Gene Activity

Cell Organization and Movement I: Microfilaments

Cell Organization and Movement II: Microtubules and Intermediate Filaments

Regulating the Eukaryotic Cell Cycle

Integrating Cells Into Tissues

Cell Birth, Lineage, and Death

Nerve Cells

Immunology

Cancer

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