Question 1

Which of the following enzymes will produce a blunt end (the cut site is indicated by the * in the recognition sequence)?

Accepted Answer

A) TaqI (T*CG
A)  
B) EagI (C*GGCCG) 
C) EcoRV (GAT*AT
C)  
D) NsiI (ATGCA*T) 
A) TaqI (T*CG
A)  
B) EagI (C*GGCCG) 
C) EcoRV (GAT*AT
C)  
D) NsiI (ATGCA*T)

Question 2

The identification of disease genes can be made more complicated because of

Accepted Answer

A) genetic heterogeneity. 
B) location of the gene on the X chromosome. 
C) polygenic traits. 
D) genetic heterogeneity and polygenic traits. 
A) genetic heterogeneity. 
B) location of the gene on the X chromosome. 
C) polygenic traits. 
D) genetic heterogeneity and polygenic traits.

Question 3

Next generation sequencing is much more efficient than the Sanger method because

Accepted Answer

A) it uses an RNA template instead of DNA. 
B) it uses gel electrophoresis to resolve end-labeled strands of DNA. 
C) it uses PCR amplification. 
D) it uses gel electrophoresis to resolve end-labeled strands of DNA,and it uses PCR amplification.
Ans.c 
A) it uses an RNA template instead of DNA. 
B) it uses gel electrophoresis to resolve end-labeled strands of DNA. 
C) it uses PCR amplification. 
D) it uses gel electrophoresis to resolve end-labeled strands of DNA,and it uses PCR amplification.
Ans.c

Question 4

To study the function of the essential cytosolic Hsc70 genes in yeast,researchers constructed a shuttle vector in which a copy of the Hsc70 gene was ligated to the GAL1 promoter.The vector was then introduced into haploid yeast cells in which all four copies of the Hsc70 genes had been disrupted.Following introduction of the vector,you would expect that

Accepted Answer

A) the yeast cells would grow on both glucose and galactose media. 
B) the yeast cells would grow on glucose but not galactose medium. 
C) the yeast cells would grow on galactose but not glucose medium. 
D) on transfer to either glucose or galactose medium,the vector-carrying cells would eventually stop growing because of insufficient Hsc70 activity. 
A) the yeast cells would grow on both glucose and galactose media. 
B) the yeast cells would grow on glucose but not galactose medium. 
C) the yeast cells would grow on galactose but not glucose medium. 
D) on transfer to either glucose or galactose medium,the vector-carrying cells would eventually stop growing because of insufficient Hsc70 activity.

Question 5

The chemical mutagen ethylmethane sulfonate (EMS)was used to treat cells,and after 24 hours of incubation,DNA was isolated from these cells and sent for sequencing.Based on the DNA sequence from untreated cells,those treated with EMS showed several of which of the following conversions?

Accepted Answer

A) G-C to A-T 
B) G-A to C-T 
C) G-T to A-C 
D) G-G to T-G 
A) G-C to A-T 
B) G-A to C-T 
C) G-T to A-C 
D) G-G to T-G

Question 6

Describe the essential features of a yeast shuttle vector.

Accepted Answer

A yeast shuttle vector is a plasmid that

Question 7

Although small hairpin RNAs are used in RNAi-mediated destruction of a target mRNA,in order for them to be effective,the double-stranded RNA must be cleaved by which of one of the following?

Accepted Answer

A) RISC 
B) RNA polymerase 
C) exonuclease 
D) dicer 
A) RISC 
B) RNA polymerase 
C) exonuclease 
D) dicer

Question 8

DNA ligase

Accepted Answer

A) synthesizes DNA from an RNA template. 
B) forms a phosphodiester bond. 
C) joins Okazaki fragments. 
D) b and c 
A) synthesizes DNA from an RNA template. 
B) forms a phosphodiester bond. 
C) joins Okazaki fragments. 
D) b and c

Question 9

Crossing of a homozygous wild type with a mutant that is heterozygous for a dominant mutation will result in F₁ progeny of which

Accepted Answer

A) all show the mutant phenotype. 
B) half show the wild-type phenotype and half show the mutant phenotype. 
C) three-fourths show the wild-type phenotype and one-fourth show the mutant phenotype. 
D) all show the wild-type phenotype. 
A) all show the mutant phenotype. 
B) half show the wild-type phenotype and half show the mutant phenotype. 
C) three-fourths show the wild-type phenotype and one-fourth show the mutant phenotype. 
D) all show the wild-type phenotype.

Question 10

Describe the properties and utility of temperature-sensitive mutations.

Accepted Answer

A temperature-sensitive mutation is a mu

Which of the following enzymes will produce a blunt end (the cut site is indicated by the * in the recognition sequence)?

The identification of disease genes can be made more complicated because of

Next generation sequencing is much more efficient than the Sanger method because

The chemical mutagen ethylmethane sulfonate (EMS)was used to treat cells,and after 24 hours of incubation,DNA was isolated from these cells and sent for sequencing.Based on the DNA sequence from untreated cells,those treated with EMS showed several of which of the following conversions?

Describe the essential features of a yeast shuttle vector.

Although small hairpin RNAs are used in RNAi-mediated destruction of a target mRNA,in order for them to be effective,the double-stranded RNA must be cleaved by which of one of the following?

DNA ligase

Crossing of a homozygous wild type with a mutant that is heterozygous for a dominant mutation will result in F₁ progeny of which

Describe the properties and utility of temperature-sensitive mutations.

Chemical Foundations

Protein Structure and Function

Culturing and Visualizing Cells

Fundamental Molecular Genetic Mechanisms

Bio-membrane Structure

Genes Genomics and Chromosomes

Transcriptional Control of Gene Expression

Post-Transcriptional Gene Control

Transmembrane Transport of Ions and Small Molecules

Cellular Energetics

Moving Proteins Into Membranes and Organelles

Vesicular Traffic, Secretion, and Endocytosis

Signal Transduction and G-Protein Coupled Receptors

Signaling Pathways That Control Gene Activity

Cell Organization and Movement I: Microfilaments

Cell Organization and Movement II: Microtubules and Intermediate Filaments

Regulating the Eukaryotic Cell Cycle

Integrating Cells Into Tissues

Cell Birth, Lineage, and Death

Nerve Cells

Immunology

Cancer

Filters

Exam 2: Molecular Genetic Techniques

Which of the following enzymes will produce a blunt end (the cut site is indicated by the * in the recognition sequence)?

The identification of disease genes can be made more complicated because of

Next generation sequencing is much more efficient than the Sanger method because

The chemical mutagen ethylmethane sulfonate (EMS)was used to treat cells,and after 24 hours of incubation,DNA was isolated from these cells and sent for sequencing.Based on the DNA sequence from untreated cells,those treated with EMS showed several of which of the following conversions?

Describe the essential features of a yeast shuttle vector.

Although small hairpin RNAs are used in RNAi-mediated destruction of a target mRNA,in order for them to be effective,the double-stranded RNA must be cleaved by which of one of the following?

DNA ligase

Crossing of a homozygous wild type with a mutant that is heterozygous for a dominant mutation will result in F₁ progeny of which

Describe the properties and utility of temperature-sensitive mutations.

Chemical Foundations

Protein Structure and Function

Culturing and Visualizing Cells

Fundamental Molecular Genetic Mechanisms

Bio-membrane Structure

Genes Genomics and Chromosomes

Transcriptional Control of Gene Expression

Post-Transcriptional Gene Control

Transmembrane Transport of Ions and Small Molecules

Cellular Energetics

Moving Proteins Into Membranes and Organelles

Vesicular Traffic, Secretion, and Endocytosis

Signal Transduction and G-Protein Coupled Receptors

Signaling Pathways That Control Gene Activity

Cell Organization and Movement I: Microfilaments

Cell Organization and Movement II: Microtubules and Intermediate Filaments

Regulating the Eukaryotic Cell Cycle

Integrating Cells Into Tissues

Cell Birth, Lineage, and Death

Nerve Cells

Immunology

Cancer

Filters