Question 1

To clone a recombinant plasmid,you would

Accepted Answer

A)  insert the plasmid into a virus and inject the virus into a human cell. 
B)  allow bacteria to take up the plasmid and copy it many times. 
C)  mix the plasmid with restriction enzymes and nucleotides. 
D)  use ligase to fuse the ends of the plasmids together. 
A)  insert the plasmid into a virus and inject the virus into a human cell. 
B)  allow bacteria to take up the plasmid and copy it many times. 
C)  mix the plasmid with restriction enzymes and nucleotides. 
D)  use ligase to fuse the ends of the plasmids together.

Question 2

Bacteria are the most common host cells for DNA cloning because they reproduce rapidly and create many copies of the recombinant plasmid.

Accepted Answer

A) True 
 B)False

Question 3

The use of DNA analysis to identify individuals is known as

Accepted Answer

A)  PCR testing. 
B)  DNA fingerprinting. 
C)  genetic modification. 
D)  cloning. 
A)  PCR testing. 
B)  DNA fingerprinting. 
C)  genetic modification. 
D)  cloning.

Question 4

The following figure shows the first cycle in polymerase chain reaction (PCR).   What is the role of the structure marked "P" in this figure?

Accepted Answer

A)  The "P" represents the heat-tolerant DNA polymerase used in PCR to copy the gene of interest. 
B)  The "P" represents a sequence of DNA complementary to parts of the gene being amplified that allows DNA polymerase to attach to the gene. 
C)  The "P" indicates where on the DNA that DNA polymerase should terminate replication. 
D)  The "P" indicates the location of the promoter for the gene being amplified. 
A)  The "P" represents the heat-tolerant DNA polymerase used in PCR to copy the gene of interest. 
B)  The "P" represents a sequence of DNA complementary to parts of the gene being amplified that allows DNA polymerase to attach to the gene. 
C)  The "P" indicates where on the DNA that DNA polymerase should terminate replication. 
D)  The "P" indicates the location of the promoter for the gene being amplified.

Question 5

In the following diagram,the electrical pulse fuses the enucleated egg from one sheep and the somatic mammary cell from another sheep.This fused cell is most similar to a ________ formed during normal sexual reproduction.

Accepted Answer

A)  somatic cell 
B)  meiotic germ cell 
C)  gamete 
D)  zygote 
A)  somatic cell 
B)  meiotic germ cell 
C)  gamete 
D)  zygote

Question 6

Tissue containing totipotent embryonic stem cells is difficult to obtain; what is the usual source?

Accepted Answer

A)  donated blastocysts produced by in vitro fertilization for women seeking fertility treatment 
B)  tissues from spontaneous miscarriage during the first trimester of pregnancy 
C)  blood and tissues extracted from the placenta after the birth of the fetus 
D)  cloned embryos produced by fusing a somatic cell with an enucleated egg cell 
A)  donated blastocysts produced by in vitro fertilization for women seeking fertility treatment 
B)  tissues from spontaneous miscarriage during the first trimester of pregnancy 
C)  blood and tissues extracted from the placenta after the birth of the fetus 
D)  cloned embryos produced by fusing a somatic cell with an enucleated egg cell

Question 7

Mammalian pluripotent stem cells can produce all

Accepted Answer

A)  cell types found in an organism. 
B)  cell types found in an organism, except the protective membranes that surround the embryo. 
C)  cell types found in the organ of their origin. 
D)  somatic cells found in an organism but not the reproductive germ cells. 
A)  cell types found in an organism. 
B)  cell types found in an organism, except the protective membranes that surround the embryo. 
C)  cell types found in the organ of their origin. 
D)  somatic cells found in an organism but not the reproductive germ cells.

Question 8

Unipotent stem cells can generate all the cells of the organ of their origin.

Accepted Answer

A) True 
 B)False

Question 9

You are shown the following result of gel electrophoresis.Column 1 represents the suspect's blood.Column 2 represents the victim's blood.Which column represents a mixture of the suspect's and victim's blood?

Accepted Answer

A)  3 
B)  4 
C)  5 
D)  A mixture is not represented in this DNA fingerprint. 
A)  3 
B)  4 
C)  5 
D)  A mixture is not represented in this DNA fingerprint.

Question 10

Bt-corn has been genetically engineered to express an insect-killing gene from the bacteria Bacillus thuringiensis.How does this genetically engineered corn benefit the environment?

Accepted Answer

A)  It provides a food source for important pollinator insects. 
B)  It decreases the amount of land necessary to grow the world's corn requirements. 
C)  It increases the amount of insecticide that needs to be used on the crops. 
D)  It decreases the amount of insecticide that needs to be used on the crops. 
A)  It provides a food source for important pollinator insects. 
B)  It decreases the amount of land necessary to grow the world's corn requirements. 
C)  It increases the amount of insecticide that needs to be used on the crops. 
D)  It decreases the amount of insecticide that needs to be used on the crops.

Question 11

In the first step of the polymerase chain reaction (PCR),the two strands of the DNA being amplified must be separated by exposing the DNA to high ________.

Accepted Answer

heat,tempe

Question 12

The DNA primers used in PCR are

Accepted Answer

A)  identical to the entire base sequence of one strand of the DNA. 
B)  produced when a gene of interest is read by restriction enzymes. 
C)  attached to the gene of interest by ligase. 
D)  complementary to DNA sequences at both ends of the DNA sequence of interest. 
A)  identical to the entire base sequence of one strand of the DNA. 
B)  produced when a gene of interest is read by restriction enzymes. 
C)  attached to the gene of interest by ligase. 
D)  complementary to DNA sequences at both ends of the DNA sequence of interest.

Question 13

Genetic engineering works only on animals and has not yet been successfully used on plants.

Accepted Answer

A) True 
 B)False

Question 14

Stem cells display two unique characteristics: the ability to ________ and ________.

Accepted Answer

A)  produce vaccines; produce insulin 
B)  cut DNA into smaller pieces; accept recombinant plasmids 
C)  express genes; synthesize proteins 
D)  differentiate into a variety of cell types; divide repeatedly 
A)  produce vaccines; produce insulin 
B)  cut DNA into smaller pieces; accept recombinant plasmids 
C)  express genes; synthesize proteins 
D)  differentiate into a variety of cell types; divide repeatedly

Question 15

Which of the following is NOT an example of a genetically modified organism?

Accepted Answer

A)  a bacterium that produces the human insulin protein 
B)  a virus used in gene therapy containing a "good copy" of a human gene 
C)  human cells isolated from a tumor and grown in a dish 
D)  a petunia plant that expresses human antibody proteins 
A)  a bacterium that produces the human insulin protein 
B)  a virus used in gene therapy containing a "good copy" of a human gene 
C)  human cells isolated from a tumor and grown in a dish 
D)  a petunia plant that expresses human antibody proteins

Question 16

The DNA for the normal hemoglobin allele and the mutant sickle-cell allele are cut with the same restriction enzyme,DdeI.The resulting DNA fragments are separated by gel electrophoresis,producing the gel shown in the following figure.Why are the patterns produced by these two alleles different?

Accepted Answer

A)  The normal hemoglobin gene is cut by two restriction enzymes, but the sickle-cell anemia allele is cut by only one restriction enzyme. 
B)  The gene for the normal allele is much larger than the gene for sickle-cell anemia. 
C)  The gene for the normal allele has a sequence recognized by DdeI, but the gene for sickle-cell anemia does not. 
D)  The sickle-cell allele has the ability to produce ligase to repair the damage done by restriction enzymes, but the normal allele does not. 
A)  The normal hemoglobin gene is cut by two restriction enzymes, but the sickle-cell anemia allele is cut by only one restriction enzyme. 
B)  The gene for the normal allele is much larger than the gene for sickle-cell anemia. 
C)  The gene for the normal allele has a sequence recognized by DdeI, but the gene for sickle-cell anemia does not. 
D)  The sickle-cell allele has the ability to produce ligase to repair the damage done by restriction enzymes, but the normal allele does not.

Exam 13: DNA Technology

To clone a recombinant plasmid,you would

Bacteria are the most common host cells for DNA cloning because they reproduce rapidly and create many copies of the recombinant plasmid.

The use of DNA analysis to identify individuals is known as

The following figure shows the first cycle in polymerase chain reaction (PCR). What is the role of the structure marked "P" in this figure?

In the following diagram,the electrical pulse fuses the enucleated egg from one sheep and the somatic mammary cell from another sheep.This fused cell is most similar to a ________ formed during normal sexual reproduction.

Tissue containing totipotent embryonic stem cells is difficult to obtain; what is the usual source?

Mammalian pluripotent stem cells can produce all

Unipotent stem cells can generate all the cells of the organ of their origin.

You are shown the following result of gel electrophoresis.Column 1 represents the suspect's blood.Column 2 represents the victim's blood.Which column represents a mixture of the suspect's and victim's blood?

Bt-corn has been genetically engineered to express an insect-killing gene from the bacteria Bacillus thuringiensis.How does this genetically engineered corn benefit the environment?

In the first step of the polymerase chain reaction (PCR),the two strands of the DNA being amplified must be separated by exposing the DNA to high ________.

The DNA primers used in PCR are

Genetic engineering works only on animals and has not yet been successfully used on plants.

Stem cells display two unique characteristics: the ability to ________ and ________.

Which of the following is NOT an example of a genetically modified organism?

The DNA for the normal hemoglobin allele and the mutant sickle-cell allele are cut with the same restriction enzyme,DdeI.The resulting DNA fragments are separated by gel electrophoresis,producing the gel shown in the following figure.Why are the patterns produced by these two alleles different?

The Nature of Science and the Characteristics of Life

The Chemistry of Life

Cell Structure and Internal Compartments

Cell Membranes, Transport, and Communication

Energy, Metabolism, and Enzymes

Photo Synthesis and Cellular Respiration

Cell Division

Cancer and Human Health

Patterns of Inheritance

Chromosomes and Human Genetics

DNA and Genes

From Gene to Protein

How Evolution Works

The Origin of Species

The Evolution of Biodiversity

Biological Diversity, Bacteria, and Archaea

Protista, Plantae, and Fungi

Animalia

The Biosphere

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Animal Behavior

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