Question 1

DNA repair cannot be done without breaking the phosphodiester backbone of DNA.

Accepted Answer

A) True 
 B)False

Question 2

Automated DNA sequencing methods that use amplification clusters of DNA fragments attached to glass slides and a unique DNA polymerase are much faster than the Sanger method.

Accepted Answer

A) True 
 B)False

Question 3

Fragments on the lagging strand are joined by the enzyme ________.

Accepted Answer

A) DNA polymerase I 
B) DNA synthase 
C) DNA primosome 
D) DNA ligase 
A) DNA polymerase I 
B) DNA synthase 
C) DNA primosome 
D) DNA ligase

Question 4

The incorporation of new nucleotides into a growing strand of DNA occurs during ________.

Accepted Answer

A) the elongation stage 
B) nucleation 
C) initiation 
D) assembly of the protein machine 
A) the elongation stage 
B) nucleation 
C) initiation 
D) assembly of the protein machine

Question 5

The replication of DNA is ________ because ________.

Accepted Answer

A) conservative;one strand of parental DNA is retained in each daughter DNA 
B) conservative;each daughter molecule has two new strands copied from the parental DNA template 
C) semiconservative;one strand of parental DNA is retained in each daughter DNA 
D) semiconservative;each daughter molecule has two new strands copied from the parental DNA template 
A) conservative;one strand of parental DNA is retained in each daughter DNA 
B) conservative;each daughter molecule has two new strands copied from the parental DNA template 
C) semiconservative;one strand of parental DNA is retained in each daughter DNA 
D) semiconservative;each daughter molecule has two new strands copied from the parental DNA template

Question 6

The lagging strand loops back through the replisome so that

Accepted Answer

A) SSB tetramers can open them. 
B) it catches up with the leading strand. 
C) both leading and laggings strands are made in the same direction. 
D) gyrase can open the helix. 
A) SSB tetramers can open them. 
B) it catches up with the leading strand. 
C) both leading and laggings strands are made in the same direction. 
D) gyrase can open the helix.

Question 7

The general excision repair pathway for DNA repair has the following order:

Accepted Answer

A) endonuclease cuts → helicase or endonuclease removes → DNA polymerase → DNA ligase 
B) DNA ligase → DNA polymerase → helicase or endonuclease removes → endonuclease cuts 
C) endonuclease cuts → DNA polymerase repairs → helicase opens up → DNA ligase 
D) endonuclease cuts → DNA ligase removes → endonuclease removes → DNA ligase 
A) endonuclease cuts → helicase or endonuclease removes → DNA polymerase → DNA ligase 
B) DNA ligase → DNA polymerase → helicase or endonuclease removes → endonuclease cuts 
C) endonuclease cuts → DNA polymerase repairs → helicase opens up → DNA ligase 
D) endonuclease cuts → DNA ligase removes → endonuclease removes → DNA ligase

Question 8

Both strands of double-stranded DNA are copied so that each daughter molecule consists of one parental strand and one newly synthesized strand.This is called ________ replication.

Accepted Answer

A) bidirectional 
B) conservative 
C) semiconservative 
D) duplicative 
A) bidirectional 
B) conservative 
C) semiconservative 
D) duplicative

Question 9

Transposons are mobile genetic elements that jump from chromosome to chromosome by

Accepted Answer

A) homologous recombination. 
B) non-homologous recombination. 
C) site specific recombination. 
D) mutation. 
A) homologous recombination. 
B) non-homologous recombination. 
C) site specific recombination. 
D) mutation.

Question 10

What is a reasonable length for an RNA primer in E.coli?

Accepted Answer

A) About 20 nucleotides. 
B) 50 nucleotides. 
C) 100 nucleotides. 
D) 1000 nucleotides. 
A) About 20 nucleotides. 
B) 50 nucleotides. 
C) 100 nucleotides. 
D) 1000 nucleotides.

Question 11

Which DNA polymerase is most responsible for chain elongation in E.coli?

Accepted Answer

A) DNA polymerase I. 
B) DNA polymerase II. 
C) DNA polymerase III. 
D) DNA polymerase IV. 
A) DNA polymerase I. 
B) DNA polymerase II. 
C) DNA polymerase III. 
D) DNA polymerase IV.

Question 12

In eukaryotes,after completion of S-phase and G2-phase,SPK (S-phase protein kinase)is broken down.This allows

Accepted Answer

A) binding of ORC (origin recognition complex). 
B) prevention of new DNA formation. 
C) the beginning of mitosis. 
D) termination of replication. 
A) binding of ORC (origin recognition complex). 
B) prevention of new DNA formation. 
C) the beginning of mitosis. 
D) termination of replication.

Question 13

DNA fingerprinting is possible for identification because each person accumulates over a hundred mutations during his or her lifetime.

Accepted Answer

A) True 
 B)False

Question 14

The purpose of SSB is to prevent single-stranded DNA from folding back on itself to form double-helical regions.

Accepted Answer

A) True 
 B)False

Question 15

The best enzyme to use in the Sanger method for DNA sequence determination is

Accepted Answer

A) DNA polymerase I. 
B) Klenow fragment of DNA polymerase I. 
C) DNA polymerase from a high temperature bacterium. 
D) SSB protein. 
A) DNA polymerase I. 
B) Klenow fragment of DNA polymerase I. 
C) DNA polymerase from a high temperature bacterium. 
D) SSB protein.

Question 16

Which proteins are responsible for the unwinding of the double-stranded DNA during replication?

Accepted Answer

A) Ligases. 
B) Helicases. 
C) Topoisomerases. 
D) Primases. 
E) Lyases. 
A) Ligases. 
B) Helicases. 
C) Topoisomerases. 
D) Primases. 
E) Lyases.

Question 17

Which molecules have repair mechanisms in most cells?

Accepted Answer

A) DNA. 
B) RNA. 
C) Proteins. 
D) DNA and proteins. 
E) DNA,RNA and proteins. 
A) DNA. 
B) RNA. 
C) Proteins. 
D) DNA and proteins. 
E) DNA,RNA and proteins.

Question 18

How many replisomes are required for one complete round of DNA replication in E.coli?

Accepted Answer

A) One 
B) Two 
C) Four 
D) More than 4 
A) One 
B) Two 
C) Four 
D) More than 4

Question 19

The primosome is composed of polypeptides including primase which is

Accepted Answer

A) DNA polymerase III. 
B) RNA dependent DNA polymerase. 
C) DNA dependent RNA polymerase. 
D) Parental DNA. 
A) DNA polymerase III. 
B) RNA dependent DNA polymerase. 
C) DNA dependent RNA polymerase. 
D) Parental DNA.

Question 20

A major difference between prokaryotic and eukaryotic DNA replication mechanisms is

Accepted Answer

A) no Okazaki fragments are made in eukaryotes. 
B) both leading and lagging strands are continuous in eukaryotes. 
C) no primer synthesis is needed in eukaryotes. 
D) the replication fork moves slower in eukaryotes. 
A) no Okazaki fragments are made in eukaryotes. 
B) both leading and lagging strands are continuous in eukaryotes. 
C) no primer synthesis is needed in eukaryotes. 
D) the replication fork moves slower in eukaryotes.

Exam 20: DNA Replication, repair, and Recombination

DNA repair cannot be done without breaking the phosphodiester backbone of DNA.

Automated DNA sequencing methods that use amplification clusters of DNA fragments attached to glass slides and a unique DNA polymerase are much faster than the Sanger method.

Fragments on the lagging strand are joined by the enzyme ________.

The incorporation of new nucleotides into a growing strand of DNA occurs during ________.

The replication of DNA is ________ because ________.

The lagging strand loops back through the replisome so that

The general excision repair pathway for DNA repair has the following order:

Both strands of double-stranded DNA are copied so that each daughter molecule consists of one parental strand and one newly synthesized strand.This is called ________ replication.

Transposons are mobile genetic elements that jump from chromosome to chromosome by

What is a reasonable length for an RNA primer in E.coli?

Which DNA polymerase is most responsible for chain elongation in E.coli?

In eukaryotes,after completion of S-phase and G2-phase,SPK (S-phase protein kinase)is broken down.This allows

DNA fingerprinting is possible for identification because each person accumulates over a hundred mutations during his or her lifetime.

The purpose of SSB is to prevent single-stranded DNA from folding back on itself to form double-helical regions.

The best enzyme to use in the Sanger method for DNA sequence determination is

Which proteins are responsible for the unwinding of the double-stranded DNA during replication?

Which molecules have repair mechanisms in most cells?

How many replisomes are required for one complete round of DNA replication in E.coli?

The primosome is composed of polypeptides including primase which is

A major difference between prokaryotic and eukaryotic DNA replication mechanisms is

Introduction to Biochemistry

Water

Amino Acids and the Primary Structures of Proteins

Proteins: Three-Dimensional Structure and Function

Properties of Enzymes

Mechanisms of Enzymes

Coenzymes and Vitamins

Carbohydrates

Lipids and Membranes

Introduction to Metabolism

Glycolysis

Gluconeogenesis, the Pentose Phosphate Pathway, and Glycogen Metabolism

The Citric Acid Cycle

Electron Transport and Atp Synthesis

Photosynthesis

Lipid Metabolism

Amino Acid Metabolism

Nucleotide Metabolism

Nucleic Acids

Transcription and RNA Processing

Protein Synthesis

Filters

The replication of DNA is because .