Question 1

Eosin Y is a counterstain that colors:

Accepted Answer

A) Red 
B) Yellow 
C) Blue 
D) Green 
A) Red 
B) Yellow 
C) Blue 
D) Green

Question 2

All of the following groups of stains contain Basic Fuschin, except:

Accepted Answer

A) Colemans Feulgen reagent,Mallory's stain, Schiff's reagent 
B) Carbol Fuschin, Gomori's stain, Shiff's reagent 
C) Mayer's Carmalum, Congo red, Bismarck Brown 
D) All of the above except B 
A) Colemans Feulgen reagent,Mallory's stain, Schiff's reagent 
B) Carbol Fuschin, Gomori's stain, Shiff's reagent 
C) Mayer's Carmalum, Congo red, Bismarck Brown 
D) All of the above except B

Question 3

A histotechnician needs to re-stain an old slide for repeat microscopy of the Pathologist. What is the proper order before re-staining and the proper reagents to be used?

Accepted Answer

A) Immerse the old slide in bleach for 24 hours, remove the cover slip, immerse in Xylene for 30 minutes, immerse in potassium permanganate for 5-10 minutes, rinse with water for 5 minutes, immerse with 5% oxalic acid for 5 minutes, rinse, and re-stain with the appropriate stain. 
B) Immerse the old slide in Xylene for 24 hours, remove the cover slip, immerse in Xylene for another 30 minutes, immerse in potassium permanganate for 5-10 minutes, rinse with water for 5 minutes, immerse in 5% oxalic acid for 5 minutes, rinse, and re-stain with the appropriate stain. 
C) Immerse the old slide in Xylene for 24 hours, remove the cover slip, immerse in Xylene for another 30 minutes, immerse in potassium permanganate for 5-10 minutes, rinse with water for 5 minutes, immerse in 5% picric acid for 5 minutes, rinse, and re-stain with the appropriate stain. 
D) Immerse the old slide in bleach for 24 hours, remove the cover slip, immersed in Xylene for 30 minutes, immerse in potassium permanganate for 5-10 minutes, rinse with water for 5 minutes, immerse in 5% glacial acetic acid for 5 minutes, rinse, and re-stain with the appropriate stain. 
A) Immerse the old slide in bleach for 24 hours, remove the cover slip, immerse in Xylene for 30 minutes, immerse in potassium permanganate for 5-10 minutes, rinse with water for 5 minutes, immerse with 5% oxalic acid for 5 minutes, rinse, and re-stain with the appropriate stain. 
B) Immerse the old slide in Xylene for 24 hours, remove the cover slip, immerse in Xylene for another 30 minutes, immerse in potassium permanganate for 5-10 minutes, rinse with water for 5 minutes, immerse in 5% oxalic acid for 5 minutes, rinse, and re-stain with the appropriate stain. 
C) Immerse the old slide in Xylene for 24 hours, remove the cover slip, immerse in Xylene for another 30 minutes, immerse in potassium permanganate for 5-10 minutes, rinse with water for 5 minutes, immerse in 5% picric acid for 5 minutes, rinse, and re-stain with the appropriate stain. 
D) Immerse the old slide in bleach for 24 hours, remove the cover slip, immersed in Xylene for 30 minutes, immerse in potassium permanganate for 5-10 minutes, rinse with water for 5 minutes, immerse in 5% glacial acetic acid for 5 minutes, rinse, and re-stain with the appropriate stain.

Question 4

Below are techniques used in staining collagen. Which of the following techniques is not used for such purpose?

Accepted Answer

A) Van Gieson's stain 
B) Masson's Trichome stain 
C) Malloy's Aniline blue stain 
D) Taenzer-Unna Orcein method 
A) Van Gieson's stain 
B) Masson's Trichome stain 
C) Malloy's Aniline blue stain 
D) Taenzer-Unna Orcein method

Question 5

Harris' solution is an alum hematoxylin. Which of the following is used to oxidize Harris' solution?

Accepted Answer

A) Mercuric chloride 
B) Sodium iodate 
C) Ferric chloride 
D) Potassium iodate 
A) Mercuric chloride 
B) Sodium iodate 
C) Ferric chloride 
D) Potassium iodate

Question 6

What is the use of a mordant?

Accepted Answer

A) To form a bridge between the stain used and the tissue to be stained. 
B) To decolorize the stained tissue. 
C) To act as a counterstain that will stain the tissue with pinkish to red and the background as blue. 
D) To combine aqueous solutions of acid and basic dyes. 
A) To form a bridge between the stain used and the tissue to be stained. 
B) To decolorize the stained tissue. 
C) To act as a counterstain that will stain the tissue with pinkish to red and the background as blue. 
D) To combine aqueous solutions of acid and basic dyes.

Question 7

This is considered as an excellent stain for acid mucopolysaccharides. What is this specific stain?

Accepted Answer

A) Aniline blue 
B) Alcian blue 
C) Celestine blue 
D) Congo Red 
A) Aniline blue 
B) Alcian blue 
C) Celestine blue 
D) Congo Red

Question 8

In what method of staining does the dye taken up by the tissue do not become washed or decolorized?

Accepted Answer

A) Regressive staining 
B) Direct staining 
C) Progressive staining 
D) Metachromatic staining 
A) Regressive staining 
B) Direct staining 
C) Progressive staining 
D) Metachromatic staining

Question 9

Name the substance that intensifies the action of the dye or stain in cell or tissue preparations.

Accepted Answer

A) Mordant 
B) Accentuator 
C) Accelerator 
D) Dye 
A) Mordant 
B) Accentuator 
C) Accelerator 
D) Dye

Question 10

What alum hematoxylin solution is chemically ripened by sodium iodate?

Accepted Answer

A) Ehrlich's hematoxylin 
B) Harris hematoxylin 
C) Cole's hematoxylin 
D) Mayer's hematoxylin 
A) Ehrlich's hematoxylin 
B) Harris hematoxylin 
C) Cole's hematoxylin 
D) Mayer's hematoxylin

Question 11

What staining solution does not belong to the group?

Accepted Answer

A) Ehrlich's hematoxylin 
B) Harris's hematoxylin 
C) Cole's hematoxylin 
D) Heidenhain's hematoxylin 
A) Ehrlich's hematoxylin 
B) Harris's hematoxylin 
C) Cole's hematoxylin 
D) Heidenhain's hematoxylin

Question 12

This is the process whereby tissue elements are stained in a definite sequence. What do you call this staining process?

Accepted Answer

A) Direct staining 
B) Indirect staining 
C) Progressive staining 
D) Regressive Staining 
A) Direct staining 
B) Indirect staining 
C) Progressive staining 
D) Regressive Staining

Question 13

Iron hematoxylin is a ferric salt used to ripen hematoxylin. Which of the following statements is not correct about iron hematoxylin?

Accepted Answer

A) It is used only for regressive staining. 
B) It uses acid-alcohol as a differentiating agent. 
C) Tissues structures are stained blackish or grayish. 
D) The two main iron hematoxylin solutions are Weigert's and Cole's hematoxylin solution. 
A) It is used only for regressive staining. 
B) It uses acid-alcohol as a differentiating agent. 
C) Tissues structures are stained blackish or grayish. 
D) The two main iron hematoxylin solutions are Weigert's and Cole's hematoxylin solution.

Question 14

In Harris hematoxylin staining solution, what indicates deterioration of its nuclear staining property?

Accepted Answer

A) Formation of precipitates 
B) Formation of dark color 
C) Both A and B 
D) None of the above 
A) Formation of precipitates 
B) Formation of dark color 
C) Both A and B 
D) None of the above

Question 15

Which type of stain is described as by far the most valuable staining reagent used by cytologists due its powerful nuclear and chromatin staining capacity?

Accepted Answer

A) Hematoxylin 
B) Cochineal dye 
C) Saffron 
D) Orcein 
A) Hematoxylin 
B) Cochineal dye 
C) Saffron 
D) Orcein

Question 16

What technique is useful in staining encapsulated fungi?

Accepted Answer

A) Southgates's mucicarmine 
B) Best carmine 
C) Langhan's iodine 
D) Gomori's aldehyde 
A) Southgates's mucicarmine 
B) Best carmine 
C) Langhan's iodine 
D) Gomori's aldehyde

Eosin Y is a counterstain that colors:

All of the following groups of stains contain Basic Fuschin, except:

A histotechnician needs to re-stain an old slide for repeat microscopy of the Pathologist. What is the proper order before re-staining and the proper reagents to be used?

Below are techniques used in staining collagen. Which of the following techniques is not used for such purpose?

Harris' solution is an alum hematoxylin. Which of the following is used to oxidize Harris' solution?

What is the use of a mordant?

This is considered as an excellent stain for acid mucopolysaccharides. What is this specific stain?

In what method of staining does the dye taken up by the tissue do not become washed or decolorized?

Name the substance that intensifies the action of the dye or stain in cell or tissue preparations.

What alum hematoxylin solution is chemically ripened by sodium iodate?

What staining solution does not belong to the group?

This is the process whereby tissue elements are stained in a definite sequence. What do you call this staining process?

Iron hematoxylin is a ferric salt used to ripen hematoxylin. Which of the following statements is not correct about iron hematoxylin?

In Harris hematoxylin staining solution, what indicates deterioration of its nuclear staining property?

Which type of stain is described as by far the most valuable staining reagent used by cytologists due its powerful nuclear and chromatin staining capacity?

What technique is useful in staining encapsulated fungi?

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Exam 5: Staining

Eosin Y is a counterstain that colors:

All of the following groups of stains contain Basic Fuschin, except:

A histotechnician needs to re-stain an old slide for repeat microscopy of the Pathologist. What is the proper order before re-staining and the proper reagents to be used?

Below are techniques used in staining collagen. Which of the following techniques is not used for such purpose?

Harris' solution is an alum hematoxylin. Which of the following is used to oxidize Harris' solution?

What is the use of a mordant?

This is considered as an excellent stain for acid mucopolysaccharides. What is this specific stain?

In what method of staining does the dye taken up by the tissue do not become washed or decolorized?

Name the substance that intensifies the action of the dye or stain in cell or tissue preparations.

What alum hematoxylin solution is chemically ripened by sodium iodate?

What staining solution does not belong to the group?

This is the process whereby tissue elements are stained in a definite sequence. What do you call this staining process?

Iron hematoxylin is a ferric salt used to ripen hematoxylin. Which of the following statements is not correct about iron hematoxylin?

In Harris hematoxylin staining solution, what indicates deterioration of its nuclear staining property?

Which type of stain is described as by far the most valuable staining reagent used by cytologists due its powerful nuclear and chromatin staining capacity?

What technique is useful in staining encapsulated fungi?

Fixation

Laboratory Operations

Microtomy

Processing and Embedding

Filters