Question 1

What is the usual range of thickness in a cut of tissue for routine histologic procedures?

Accepted Answer

A) 0)5 - 1.0 microns 
B) 1)0 - 2.0 microns 
C) 3)0 - 4.5 microns 
D) 4)0 - 6.0 microns 
A) 0)5 - 1.0 microns 
B) 1)0 - 2.0 microns 
C) 3)0 - 4.5 microns 
D) 4)0 - 6.0 microns D

Question 2

What do you call the process that involves the removal of the burr or irregularities that are formed during honing?

Accepted Answer

A) Stropping 
B) Honing 
C) Sharpening 
D) Cutting 
A) Stropping 
B) Honing 
C) Sharpening 
D) Cutting A

Question 3

Chino understands that the following are precautions to observe during stropping, except:

Accepted Answer

A) Vegetable oil should be applied on the surface of the strop. 
B) Pressure applied during the initial strokes of stropping should be relatively light and gentle. 
C) Stopping in a quick and fast manner should be avoided. 
D) Mineral oil is never used since it damages the leather. 
A) Vegetable oil should be applied on the surface of the strop. 
B) Pressure applied during the initial strokes of stropping should be relatively light and gentle. 
C) Stopping in a quick and fast manner should be avoided. 
D) Mineral oil is never used since it damages the leather. A

Question 4

In what angle is the knife usually tilted in the microtome?

Accepted Answer

A) 35-45 degrees 
B) 25-35 degrees 
C) 15-25 degrees 
D) 0 -15 degrees 
A) 35-45 degrees 
B) 25-35 degrees 
C) 15-25 degrees 
D) 0 -15 degrees

Question 5

The machine or instrument used for cutting sections is known as the:

Accepted Answer

A) Knife 
B) Block holder 
C) Microtome 
D) Ratchet feed wheel 
A) Knife 
B) Block holder 
C) Microtome 
D) Ratchet feed wheel

Question 6

Which of the following knives is recommended for frozen sections or for cutting extremely hard and tough specimens?

Accepted Answer

A) Biconcave knife 
B) Plane-Concave knife 
C) Plane-wedge knife 
D) None of the above 
A) Biconcave knife 
B) Plane-Concave knife 
C) Plane-wedge knife 
D) None of the above

Question 7

What type of microtome is used for cutting celloidin embedded sections?

Accepted Answer

A) Rotary microtome 
B) Rocking microtome 
C) Base-sledge microtome 
D) Freezing microtome 
A) Rotary microtome 
B) Rocking microtome 
C) Base-sledge microtome 
D) Freezing microtome

Question 8

What type of microtome is used for cutting serial sections of large blocks of paraffin-embedded tissue?

Accepted Answer

A) Rotary microtome 
B) Rocking microtome 
C) Sliding microtome 
D) Freezing microtome 
A) Rotary microtome 
B) Rocking microtome 
C) Sliding microtome 
D) Freezing microtome

Question 9

What type of microtome is used for cutting un-embedded tissue sections?

Accepted Answer

A) Rotary microtome 
B) Rocking microtome 
C) Sliding microtome 
D) Freezing microtome 
A) Rotary microtome 
B) Rocking microtome 
C) Sliding microtome 
D) Freezing microtome

Question 10

All of the above statements are true about the freezing microtome, except:

Accepted Answer

A) The freezing microtome was invented by Minot in 1848. 
B) The freezing microtome was invented by Queckett in 1848. 
C) The freezing microtome's stage for block holder is hallow and perforated around its perimeter. 
D) The freezing microtome can cut un-embedded tissue sections. 
A) The freezing microtome was invented by Minot in 1848. 
B) The freezing microtome was invented by Queckett in 1848. 
C) The freezing microtome's stage for block holder is hallow and perforated around its perimeter. 
D) The freezing microtome can cut un-embedded tissue sections.

Question 11

In the cold knife procedure, there is a term called as "Dew Line". What do you think is the meaning of this?

Accepted Answer

A) Different temperatures between the tissue and the knife 
B) The point at which sections may then be cut at 10 microns of thickness 
C) Optimum condition for sectioning 
D) Freezing the specimen 
A) Different temperatures between the tissue and the knife 
B) The point at which sections may then be cut at 10 microns of thickness 
C) Optimum condition for sectioning 
D) Freezing the specimen

Question 12

Bryan must take note that one of the following is true about the precautions during honing.

Accepted Answer

A) The hone should be of a certain length at 8 cm by 3 cm to allow the entire knife edge to be sharpened in one stroke. 
B) The pressure exerted on the knife should be kept at a steady and gentle manner to avoid it from rocking. 
C) The hone shall always be lubricated with cold, soapy water before its usage. 
D) The fluid used for washing must be flowing in a slow motion so that chips of metal are effectively removed. 
A) The hone should be of a certain length at 8 cm by 3 cm to allow the entire knife edge to be sharpened in one stroke. 
B) The pressure exerted on the knife should be kept at a steady and gentle manner to avoid it from rocking. 
C) The hone shall always be lubricated with cold, soapy water before its usage. 
D) The fluid used for washing must be flowing in a slow motion so that chips of metal are effectively removed.

Question 13

Which of the following knives is recommended for Celloidin embedded tissue blocks on a sliding microtome?

Accepted Answer

A) Biconcave knife 
B) Plane-Concave knife 
C) Plane-wedge knife 
D) None of the above 
A) Biconcave knife 
B) Plane-Concave knife 
C) Plane-wedge knife 
D) None of the above

Question 14

In cutting the tissue block, a hole is observed in the section. What do you think is the reason for this?

Accepted Answer

A) Sections are too thin 
B) Incomplete dehydration, clearing or infiltration of tissue with wax 
C) Sections are too thick 
D) Dirt was formed in the embedding medium 
A) Sections are too thin 
B) Incomplete dehydration, clearing or infiltration of tissue with wax 
C) Sections are too thick 
D) Dirt was formed in the embedding medium

Question 15

Don recalls that the following items are also needed during microtomy, aside from:

Accepted Answer

A) Waterbath 
B) Clean slides 
C) Permanent marker 
D) Drying oven 
A) Waterbath 
B) Clean slides 
C) Permanent marker 
D) Drying oven

Question 16

Which of the following sharpening stones or hones gives a more polishing effect?

Accepted Answer

A) Belgium Yellow stone 
B) Arkansas stone 
C) Fine Carborundum stone 
D) Niether A or B 
A) Belgium Yellow stone 
B) Arkansas stone 
C) Fine Carborundum stone 
D) Niether A or B

Question 17

This is the type of microtome that is used for cutting sections for Electron microscopy.

Accepted Answer

A) Rotary microtome 
B) Rocking microtome 
C) Ultrathin microtome 
D) Freezing microtome 
A) Rotary microtome 
B) Rocking microtome 
C) Ultrathin microtome 
D) Freezing microtome

Question 18

This microtome was developed by Adams in 1789.

Accepted Answer

A) Rotary microtome 
B) Sliding microtome 
C) Rocking microtome 
D) Freezing microtome 
A) Rotary microtome 
B) Sliding microtome 
C) Rocking microtome 
D) Freezing microtome

Question 19

All of the following statements are true about why lengthwise vertical scratches are seen on the sections, except:

Accepted Answer

A) The knife is sharp 
B) Dirty embedding medium 
C) The tilt of the knife is too great 
D) Nicks on the edge of the knife 
A) The knife is sharp 
B) Dirty embedding medium 
C) The tilt of the knife is too great 
D) Nicks on the edge of the knife

Question 20

This is the specific type of microtome that was invented by Paldwell Trefall in 1881?

Accepted Answer

A) Rotary microtome 
B) Sliding microtome 
C) Rocking microtome 
D) Freezing microtome 
A) Rotary microtome 
B) Sliding microtome 
C) Rocking microtome 
D) Freezing microtome

What is the usual range of thickness in a cut of tissue for routine histologic procedures?

What do you call the process that involves the removal of the burr or irregularities that are formed during honing?

Chino understands that the following are precautions to observe during stropping, except:

In what angle is the knife usually tilted in the microtome?

The machine or instrument used for cutting sections is known as the:

Which of the following knives is recommended for frozen sections or for cutting extremely hard and tough specimens?

What type of microtome is used for cutting celloidin embedded sections?

What type of microtome is used for cutting serial sections of large blocks of paraffin-embedded tissue?

What type of microtome is used for cutting un-embedded tissue sections?

All of the above statements are true about the freezing microtome, except:

In the cold knife procedure, there is a term called as "Dew Line". What do you think is the meaning of this?

Bryan must take note that one of the following is true about the precautions during honing.

Which of the following knives is recommended for Celloidin embedded tissue blocks on a sliding microtome?

In cutting the tissue block, a hole is observed in the section. What do you think is the reason for this?

Don recalls that the following items are also needed during microtomy, aside from:

Which of the following sharpening stones or hones gives a more polishing effect?

This is the type of microtome that is used for cutting sections for Electron microscopy.

This microtome was developed by Adams in 1789.

All of the following statements are true about why lengthwise vertical scratches are seen on the sections, except:

This is the specific type of microtome that was invented by Paldwell Trefall in 1881?

Fixation

Laboratory Operations

Processing and Embedding

Staining

Filters

Exam 3: Microtomy

What is the usual range of thickness in a cut of tissue for routine histologic procedures?

What do you call the process that involves the removal of the burr or irregularities that are formed during honing?

Chino understands that the following are precautions to observe during stropping, except:

In what angle is the knife usually tilted in the microtome?

The machine or instrument used for cutting sections is known as the:

Which of the following knives is recommended for frozen sections or for cutting extremely hard and tough specimens?

What type of microtome is used for cutting celloidin embedded sections?

What type of microtome is used for cutting serial sections of large blocks of paraffin-embedded tissue?

What type of microtome is used for cutting un-embedded tissue sections?

All of the above statements are true about the freezing microtome, except:

In the cold knife procedure, there is a term called as "Dew Line". What do you think is the meaning of this?

Bryan must take note that one of the following is true about the precautions during honing.

Which of the following knives is recommended for Celloidin embedded tissue blocks on a sliding microtome?

In cutting the tissue block, a hole is observed in the section. What do you think is the reason for this?

Don recalls that the following items are also needed during microtomy, aside from:

Which of the following sharpening stones or hones gives a more polishing effect?

This is the type of microtome that is used for cutting sections for Electron microscopy.

This microtome was developed by Adams in 1789.

All of the following statements are true about why lengthwise vertical scratches are seen on the sections, except:

This is the specific type of microtome that was invented by Paldwell Trefall in 1881?

Fixation

Laboratory Operations

Processing and Embedding

Staining

Filters