Question 1

With which organelle membrane would you expect to find the receptor protein importin?

Accepted Answer

A) nucleus 
B) chloroplast 
C) plastid 
D) mitochondrion 
E) nucleolus 
A) nucleus 
B) chloroplast 
C) plastid 
D) mitochondrion 
E) nucleolus

Question 2

Using the Sanger method of DNA sequencing, results in the figure below are obtained following gel electrophoresis of the DNA fragments.   A total of 21 nucleotides has been sequenced. What is the sequence of these nucleotides?

Accepted Answer

A) 5'- ACCGTTTGCAGAACGTTCCCA- 3' 
B) 5'- ACCCTTGCAAGACGTTTGCCA- 3' 
C) 5'- ACCCACGTTAAAGACCCGTTT- 3' 
D) 5'- AAAAACCCCCCCGGGGTTTTT- 3' 
E) none of the above 
A) 5'- ACCGTTTGCAGAACGTTCCCA- 3' 
B) 5'- ACCCTTGCAAGACGTTTGCCA- 3' 
C) 5'- ACCCACGTTAAAGACCCGTTT- 3' 
D) 5'- AAAAACCCCCCCGGGGTTTTT- 3' 
E) none of the above

Question 3

A variety of E. coli clones have been isolated, the DNA has been extracted, digested with restriction enzymes, denatured, separated by gel electrophoresis, and transferred to nylon membrane by the Southern blot procedure. A radioactive probe is used that hybridizes with a gene of interest. The DNA corresponding to a single clone yields a single DNA band after probing the blot. Based on this information only,

Accepted Answer

A) the gene of interest is located entirely on the DNA band visualized. 
B) only the one clone described has the gene of interest. 
C) all of the clones tested, except the one described, have the gene of interest. 
D) the part of the gene recognized by the probe is located on the DNA band visualized. 
E) both B and D 
A) the gene of interest is located entirely on the DNA band visualized. 
B) only the one clone described has the gene of interest. 
C) all of the clones tested, except the one described, have the gene of interest. 
D) the part of the gene recognized by the probe is located on the DNA band visualized. 
E) both B and D

Question 4

Which of the following is the correct order of the levels of DNA packaging in eukaryotic chromosomes?

Accepted Answer

A) nucleosome -looped domains -chromatin fiber -heterochromatin 
B) nucleosome -chromatin fiber -looped domains -heterochromatin 
C) chromatin fiber -nucleosome -looped domains -heterochromatin 
D) heterochromatin -nucleosome -chromatin fiber -looped domains 
E) chromatin fiber -heterochromatin -nucleosome -looped domains 
A) nucleosome -looped domains -chromatin fiber -heterochromatin 
B) nucleosome -chromatin fiber -looped domains -heterochromatin 
C) chromatin fiber -nucleosome -looped domains -heterochromatin 
D) heterochromatin -nucleosome -chromatin fiber -looped domains 
E) chromatin fiber -heterochromatin -nucleosome -looped domains

Question 5

In 1928, Frederick Griffith's experiments with Streptococcus pneumoniae suggested that

Accepted Answer

A) DNA was conclusively the molecule of heredity. 
B) attenuated R (rough)bacteria easily revert to the wild type (virulent). 
C) bacteria do not incorporate the DNA of other bacteria. 
D) heat- killed bacteria could somehow "transform" live bacteria. 
E) all of the above 
A) DNA was conclusively the molecule of heredity. 
B) attenuated R (rough)bacteria easily revert to the wild type (virulent). 
C) bacteria do not incorporate the DNA of other bacteria. 
D) heat- killed bacteria could somehow "transform" live bacteria. 
E) all of the above

Question 6

The specific field of study that examines the gene products of a genome is known as

Accepted Answer

A) bioinformatics. 
B) genomics. 
C) annotation. 
D) proteomics. 
E) none of the above 
A) bioinformatics. 
B) genomics. 
C) annotation. 
D) proteomics. 
E) none of the above

Question 7

Eukaryotic- like (possessing a defined nucleus)cells were obtained from a Martian soil sample. The nucleus was carefully isolated and the chromatin obtained. The fibers had a "beads on a string" appearance, suggesting some type of "histones." Following digestion with micrococcal nuclease, protein removal, and gel electrophoresis, DNA fragments were obtained that are multiples of 280 base pairs in length. Longer incubation with micrococcal nuclease prior to protein removal results in fragments 200 base pairs in length. The amount of DNA that is wrapped around the "core histones" is therefore

Accepted Answer

A) 480 base pairs in length. 
B) 200 base pairs in length. 
C) 360 base pairs in length. 
D) 80 base pairs in length. 
E) Not enough information is given to determine the answer. 
A) 480 base pairs in length. 
B) 200 base pairs in length. 
C) 360 base pairs in length. 
D) 80 base pairs in length. 
E) Not enough information is given to determine the answer.

Question 8

The nucleolus

Accepted Answer

A) is surrounded by a thin nucleolar membrane. 
B) contains the NOR of rRNA genes. 
C) synthesizes ribosomal proteins. 
D) is found one per cell in eukaryotes. 
E) all of the above 
A) is surrounded by a thin nucleolar membrane. 
B) contains the NOR of rRNA genes. 
C) synthesizes ribosomal proteins. 
D) is found one per cell in eukaryotes. 
E) all of the above

Question 9

In the Sanger method of DNA sequencing, incorporation of the nucleotides stops because

Accepted Answer

A) the dideoxynucleotide analogs have no 2'- OH group to continue DNA synthesis. 
B) the dideoxynucleotide analogs have no 3'- OH group to continue DNA synthesis. 
C) the mixture is heated to 92°C to stop DNA synthesis at specific time intervals. 
D) the dideoxynucleotide analogs are in greater concentration than the actual nucleotides. 
E) the dideoxynucleotide analogs have no 5'- OH group to continue DNA synthesis. 
A) the dideoxynucleotide analogs have no 2'- OH group to continue DNA synthesis. 
B) the dideoxynucleotide analogs have no 3'- OH group to continue DNA synthesis. 
C) the mixture is heated to 92°C to stop DNA synthesis at specific time intervals. 
D) the dideoxynucleotide analogs are in greater concentration than the actual nucleotides. 
E) the dideoxynucleotide analogs have no 5'- OH group to continue DNA synthesis.

Question 10

DNA isolated from Aspergillis has an adenine content of 25%. Based upon this information, what is the %G + C within the Aspergillis DNA?

Accepted Answer

A) 50% 
B) 75% 
C) 0% 
D) 25% 
E) Answer cannot be determined from this information. 
A) 50% 
B) 75% 
C) 0% 
D) 25% 
E) Answer cannot be determined from this information.

Question 11

Which of the following are components of Chargaff's rules of bases?

Accepted Answer

A) purines = pyrimidines 
B) C + T = A + G 
C) G = C 
D) A = T 
E) all of the above 
A) purines = pyrimidines 
B) C + T = A + G 
C) G = C 
D) A = T 
E) all of the above

Question 12

Avery, MacLeod, and McCarty (1944)demonstrated that DNA was the molecule of heredity by treating heat killed Streptococcus pneumoniae S (smooth)strain with DNase, thereby preventing transfer of the "transforming substance" from the killed S strain to the live R (rough)strain when the two were mixed together and injected into mice. Another way that one could demonstrate transformation in bacteria would be to extract DNA from

Accepted Answer

A) an ampicillin- sensitive strain and mix with cells of an ampicillin- resistant strain. 
B) both S and R strains and mix to allow recombination to take place. 
C) a S strain and mix with cells of a R strain. 
D) a R strain and mix with cells of a S strain. 
E) none of the above 
A) an ampicillin- sensitive strain and mix with cells of an ampicillin- resistant strain. 
B) both S and R strains and mix to allow recombination to take place. 
C) a S strain and mix with cells of a R strain. 
D) a R strain and mix with cells of a S strain. 
E) none of the above

Question 13

In experiments designed as those of Hershey and Chase (1952), 35S was added to virus replicating within its bacterial host. The new virions were carefully isolated and used to infect fresh bacterial cells in the absence of any radioisotopes. Where would you expect to find the 35S radioisotope?

Accepted Answer

A) in the nucleic acid of the new virions 
B) in the bacterial DNA 
C) in the protein coat of the new virions 
D) in the phage ghosts 
E) in the bacterial matrix proteins 
A) in the nucleic acid of the new virions 
B) in the bacterial DNA 
C) in the protein coat of the new virions 
D) in the phage ghosts 
E) in the bacterial matrix proteins

Question 14

demonstrated that there were equivalent amounts of adenine and thymine, and equivalent amounts of guanine and cytosine.

Accepted Answer

The answer of demonstrated that there were equivalent amounts of...

With which organelle membrane would you expect to find the receptor protein importin?

Using the Sanger method of DNA sequencing, results in the figure below are obtained following gel electrophoresis of the DNA fragments. A total of 21 nucleotides has been sequenced. What is the sequence of these nucleotides?

Which of the following is the correct order of the levels of DNA packaging in eukaryotic chromosomes?

In 1928, Frederick Griffith's experiments with Streptococcus pneumoniae suggested that

The specific field of study that examines the gene products of a genome is known as

The nucleolus

In the Sanger method of DNA sequencing, incorporation of the nucleotides stops because

DNA isolated from Aspergillis has an adenine content of 25%. Based upon this information, what is the %G + C within the Aspergillis DNA?

Which of the following are components of Chargaff's rules of bases?

In experiments designed as those of Hershey and Chase (1952), 35S was added to virus replicating within its bacterial host. The new virions were carefully isolated and used to infect fresh bacterial cells in the absence of any radioisotopes. Where would you expect to find the 35S radioisotope?

demonstrated that there were equivalent amounts of adenine and thymine, and equivalent amounts of guanine and cytosine.

A Preview of the Cell

The Chemistry of the Cell

The Macromolecules of the Cell

Cells and Organelles

Bioenergetics: The Flow of Energy in the Cell

Enzymes: The Catalysts of Life

Membranes: Their Structure, Function, and Chemistry

Transport Across Membranes: Overcoming the Permeability Barrier

Chemotrophic Energy Metabolism: Glycolysis and Fermentation

Chemotrophic Energy Metabolism: Aerobic Respiration

Phototrophic Energy Metabolism: Photosynthesis

The Endomembrane System and Peroxisomes

Signal Transduction Mechanisms: I Electrical and Synaptic Signaling in Neurons

Signal Transduction Mechanisms: Ii Messengers and Receptors

Cytoskeletal Systems

Cellular Movement: Motility and Contractility

Beyond the Cell: Cell Adhesion, Cell Junctions, and Extracellular Structures

The Cell Cycle, Dna Replication, and Mitosis

Sexual Reproduction, Meiosis, and Genetic Recombination

Gene Expression: I the Genetic Code and Transcription

Gene Expression: II Protein Synthesis and Sorting

The Regulation of Gene Expression

Cancer Cells

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Exam 18: The Structural Basis of Cellular Information: DNA, Chromosomes, and the Nucleus

With which organelle membrane would you expect to find the receptor protein importin?

Using the Sanger method of DNA sequencing, results in the figure below are obtained following gel electrophoresis of the DNA fragments. A total of 21 nucleotides has been sequenced. What is the sequence of these nucleotides?

Which of the following is the correct order of the levels of DNA packaging in eukaryotic chromosomes?

In 1928, Frederick Griffith's experiments with Streptococcus pneumoniae suggested that

The specific field of study that examines the gene products of a genome is known as

The nucleolus

In the Sanger method of DNA sequencing, incorporation of the nucleotides stops because

DNA isolated from Aspergillis has an adenine content of 25%. Based upon this information, what is the %G + C within the Aspergillis DNA?

Which of the following are components of Chargaff's rules of bases?

In experiments designed as those of Hershey and Chase (1952), 35S was added to virus replicating within its bacterial host. The new virions were carefully isolated and used to infect fresh bacterial cells in the absence of any radioisotopes. Where would you expect to find the 35S radioisotope?

demonstrated that there were equivalent amounts of adenine and thymine, and equivalent amounts of guanine and cytosine.

A Preview of the Cell

The Chemistry of the Cell

The Macromolecules of the Cell

Cells and Organelles

Bioenergetics: The Flow of Energy in the Cell

Enzymes: The Catalysts of Life

Membranes: Their Structure, Function, and Chemistry

Transport Across Membranes: Overcoming the Permeability Barrier

Chemotrophic Energy Metabolism: Glycolysis and Fermentation

Chemotrophic Energy Metabolism: Aerobic Respiration

Phototrophic Energy Metabolism: Photosynthesis

The Endomembrane System and Peroxisomes

Signal Transduction Mechanisms: I Electrical and Synaptic Signaling in Neurons

Signal Transduction Mechanisms: Ii Messengers and Receptors

Cytoskeletal Systems

Cellular Movement: Motility and Contractility

Beyond the Cell: Cell Adhesion, Cell Junctions, and Extracellular Structures

The Cell Cycle, Dna Replication, and Mitosis

Sexual Reproduction, Meiosis, and Genetic Recombination

Gene Expression: I the Genetic Code and Transcription

Gene Expression: II Protein Synthesis and Sorting

The Regulation of Gene Expression

Cancer Cells

Filters