Question 1

What is the type of each eukaryotic protein that primarily transcribes mRNA, tRNA, and rRNA, respectively?

Accepted Answer

A)  RNA Polymerase I, II, III 
B)  RNA Polymerase II, III, I 
C)  RNA Polymerase I, III, II 
D)  RNA Polymerase II, I, III 
E)  RNA Polymerase III, II, I 
A)  RNA Polymerase I, II, III 
B)  RNA Polymerase II, III, I 
C)  RNA Polymerase I, III, II 
D)  RNA Polymerase II, I, III 
E)  RNA Polymerase III, II, I

Question 2

In intrinsic termination, inverted repeat DNA sequences followed immediately by a string of ________ produce an mRNA stem- loop, followed by a string of ________.

Accepted Answer

The answer of In intrinsic termination, inverted repeat DNA sequences...

Question 3

In a given bacterium, transcription of housekeeping genes is normal, but genes involved in nitrogen metabolism, stress, and chemotaxis are disrupted. Which sigma subunit is INTACT?

Accepted Answer

A)  σ32 
B)  σ28 
C)  σ70 
D)  σ54 
E)  σ35 
A)  σ32 
B)  σ28 
C)  σ70 
D)  σ54 
E)  σ35

Question 4

If transcription of this gene occurs from left to right in the accompanying diagram, which DNA strand is the CODING (non- template) strand?

Accepted Answer

A)  impossible to determine 
B)  B 
C)  C 
D)  D 
E)  E 
A)  impossible to determine 
B)  B 
C)  C 
D)  D 
E)  E

Question 5

Prokaryotes and eukaryotes produce which of the following types of RNA?

Accepted Answer

A)  siRNA, tRNA, miRNA 
B)  rRNA, siRNA, snRNA 
C)  mRNA, gRNA, siRNA 
D)  miRNA, rRNA, snRNA 
E)  mRNA, tRNA, rRNA 
A)  siRNA, tRNA, miRNA 
B)  rRNA, siRNA, snRNA 
C)  mRNA, gRNA, siRNA 
D)  miRNA, rRNA, snRNA 
E)  mRNA, tRNA, rRNA

Question 6

What defines the end of a eukaryotic gene?

Accepted Answer

A)  A 3' UTR of at least 25 nucleotides recruits an RNase that cleaves the pre- mRNA. 
B)  Presence of a polyadenylation signal sequence leads to cleavage of the pre- mRNA. 
C)  A stem- loop structure in the transcriptional terminator region stalls the RNA polymerase. 
D)  There is no clearly defined end to eukaryotic genes, unlike for bacterial genes. 
E)  Presence of a stop codon leads to RNA polymerase stalling and ceasing transcription. 
A)  A 3' UTR of at least 25 nucleotides recruits an RNase that cleaves the pre- mRNA. 
B)  Presence of a polyadenylation signal sequence leads to cleavage of the pre- mRNA. 
C)  A stem- loop structure in the transcriptional terminator region stalls the RNA polymerase. 
D)  There is no clearly defined end to eukaryotic genes, unlike for bacterial genes. 
E)  Presence of a stop codon leads to RNA polymerase stalling and ceasing transcription.

Question 7

Transcription of inverted repeats produces an mRNA with complementary segments that fold to form what type of secondary structure?

Accepted Answer

stem- loop

Question 8

Prokaryotes have a - 10 and - 35 consensus sequence in their promoter. What are the three eukaryotic promoter sequence elements (or "boxes")?

Accepted Answer

GC- rich,

Question 9

Which type of research technique was used to track newly synthesized RNA within a eukaryotic cell?

Accepted Answer

A)  DNA footprint protection assay 
B)  in situ hybridization 
C)  pulse- chase 
D)  Southern blotting 
E)  band shift assay 
A)  DNA footprint protection assay 
B)  in situ hybridization 
C)  pulse- chase 
D)  Southern blotting 
E)  band shift assay

Question 10

Which type of RNA interacts with nuclear proteins to form a ribonucleoprotein complex responsible for intron removal?

Accepted Answer

The answer of Which type of RNA interacts with nuclear...

Question 11

What is the role of a promoter region of a gene?

Accepted Answer

A)  Protect the gene from mutations in intergenic regions. 
B)  Recruit transcription factors that form the initiation complex. 
C)  Recruit RNA Polymerase to the transcriptional start site. 
D)  Recruit rho protein to assist in transcription. 
E)  Serve as the original region of transcription of a gene. 
A)  Protect the gene from mutations in intergenic regions. 
B)  Recruit transcription factors that form the initiation complex. 
C)  Recruit RNA Polymerase to the transcriptional start site. 
D)  Recruit rho protein to assist in transcription. 
E)  Serve as the original region of transcription of a gene.

Question 12

Which enzyme is required to initiate 5' capping of eukaryotic mRNA transcripts by removing the terminal phosphate group?

Accepted Answer

A)  phosphodiesterase 
B)  adenylyl cyclase 
C)  ribozyme 
D)  methyl transferase 
E)  guanylyl transferase 
A)  phosphodiesterase 
B)  adenylyl cyclase 
C)  ribozyme 
D)  methyl transferase 
E)  guanylyl transferase

Question 13

What is the significance of the open complex when the RNA Polymerase binds the DNA?

Accepted Answer

A)  The growing RNA molecule can now fit inside the active site of the RNA Polymerase. 
B)  It permits transcription factors to bind to the RNA Polymerase. 
C)  It assists with propagation of the RNA Polymerase along the DNA helix. 
D)  The RNA Polymerase binds the single- stranded coding strand in its active site. 
E)  The RNA Polymerase binds the single- stranded template strand in its active site. 
A)  The growing RNA molecule can now fit inside the active site of the RNA Polymerase. 
B)  It permits transcription factors to bind to the RNA Polymerase. 
C)  It assists with propagation of the RNA Polymerase along the DNA helix. 
D)  The RNA Polymerase binds the single- stranded coding strand in its active site. 
E)  The RNA Polymerase binds the single- stranded template strand in its active site.

Question 14

What is the general name for the components of the spliceosome, which removes introns from mRNAs?

Accepted Answer

A)  small interfering RNA enhancers 
B)  small nuclear ribonucleoproteins 
C)  lariat intronic nucleolar proteins 
D)  microRNA activators 
E)  branch point adenine recognition proteins 
A)  small interfering RNA enhancers 
B)  small nuclear ribonucleoproteins 
C)  lariat intronic nucleolar proteins 
D)  microRNA activators 
E)  branch point adenine recognition proteins

What is the type of each eukaryotic protein that primarily transcribes mRNA, tRNA, and rRNA, respectively?

In intrinsic termination, inverted repeat DNA sequences followed immediately by a string of produce an mRNA stem- loop, followed by a string of .

In a given bacterium, transcription of housekeeping genes is normal, but genes involved in nitrogen metabolism, stress, and chemotaxis are disrupted. Which sigma subunit is INTACT?

If transcription of this gene occurs from left to right in the accompanying diagram, which DNA strand is the CODING (non- template) strand?

Prokaryotes and eukaryotes produce which of the following types of RNA?

What defines the end of a eukaryotic gene?

Transcription of inverted repeats produces an mRNA with complementary segments that fold to form what type of secondary structure?

Prokaryotes have a - 10 and - 35 consensus sequence in their promoter. What are the three eukaryotic promoter sequence elements (or "boxes")?

Which type of research technique was used to track newly synthesized RNA within a eukaryotic cell?

Which type of RNA interacts with nuclear proteins to form a ribonucleoprotein complex responsible for intron removal?

What is the role of a promoter region of a gene?

Which enzyme is required to initiate 5' capping of eukaryotic mRNA transcripts by removing the terminal phosphate group?

What is the significance of the open complex when the RNA Polymerase binds the DNA?

What is the general name for the components of the spliceosome, which removes introns from mRNAs?

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Inheritance Patterns of Single Genes and Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophage

Dna Structure and Replication

The Molecular Biology of Translation

Chromosome Chapter Title Tbd

Gene Mutation, Dna Repair, and Homologous Recombination

Chromosome Chapter Title Tbd

Regulation of Gene Expression in Bacteria and Bacteriophage

Regulation of Gene Expression in Eukaryotes

Analysis of Gene Function by Forward Genetics and Reverse Genetics

Recombinant Dna Technology and Its Applications

Genomics: Genetics From a Whole-Genome Perspective

Organelle Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution at the Population, Species, and Molecular Levels

Filters

Exam 8: Molecular Biology of Transcription and Rna Processing

What is the type of each eukaryotic protein that primarily transcribes mRNA, tRNA, and rRNA, respectively?

In intrinsic termination, inverted repeat DNA sequences followed immediately by a string of ________ produce an mRNA stem- loop, followed by a string of ________.

In a given bacterium, transcription of housekeeping genes is normal, but genes involved in nitrogen metabolism, stress, and chemotaxis are disrupted. Which sigma subunit is INTACT?

If transcription of this gene occurs from left to right in the accompanying diagram, which DNA strand is the CODING (non- template) strand?

Prokaryotes and eukaryotes produce which of the following types of RNA?

What defines the end of a eukaryotic gene?

Transcription of inverted repeats produces an mRNA with complementary segments that fold to form what type of secondary structure?

Prokaryotes have a - 10 and - 35 consensus sequence in their promoter. What are the three eukaryotic promoter sequence elements (or "boxes")?

Which type of research technique was used to track newly synthesized RNA within a eukaryotic cell?

Which type of RNA interacts with nuclear proteins to form a ribonucleoprotein complex responsible for intron removal?

What is the role of a promoter region of a gene?

Which enzyme is required to initiate 5' capping of eukaryotic mRNA transcripts by removing the terminal phosphate group?

What is the significance of the open complex when the RNA Polymerase binds the DNA?

What is the general name for the components of the spliceosome, which removes introns from mRNAs?

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Inheritance Patterns of Single Genes and Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophage

Dna Structure and Replication

The Molecular Biology of Translation

Chromosome Chapter Title Tbd

Gene Mutation, Dna Repair, and Homologous Recombination

Chromosome Chapter Title Tbd

Regulation of Gene Expression in Bacteria and Bacteriophage

Regulation of Gene Expression in Eukaryotes

Analysis of Gene Function by Forward Genetics and Reverse Genetics

Recombinant Dna Technology and Its Applications

Genomics: Genetics From a Whole-Genome Perspective

Organelle Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution at the Population, Species, and Molecular Levels

Filters

In intrinsic termination, inverted repeat DNA sequences followed immediately by a string of produce an mRNA stem- loop, followed by a string of .