Question 1

What proteins aid in the recognition of the promoter sequence and binding of RNA polyme in eukaryotes?

Accepted Answer

transcript

Question 2

Describe how band/mobility shift assays and DNA footprint protection assays can be used to identify promoter regions in DNA.

Accepted Answer

A band/mobility shift assay uses two ide

Question 3

Intron self- splicing occurs when two________ reactions excise the intron and allow exons to ligate.

Accepted Answer

The answer of Intron self- splicing occurs when two________ reactions...

Question 4

What must eukaryotes do to initiate transcription of a gene?

Accepted Answer

A)  Recruit general transcription factors to produce an open complex and then recruit RNA Polymerase. 
B)  Bind RNA Polymerase to displace histone proteins that binding DNA in the promoter region. 
C)  Bind transcription factors from enhancer sequences to the RNA Polymerase. 
D)  Recruit the transcription factors and RNA Polymerase that compose the pre- initiation complex. 
E)  Open the DNA template and then bind RNA Polymerase at the transcriptional initiation site. 
A)  Recruit general transcription factors to produce an open complex and then recruit RNA Polymerase. 
B)  Bind RNA Polymerase to displace histone proteins that binding DNA in the promoter region. 
C)  Bind transcription factors from enhancer sequences to the RNA Polymerase. 
D)  Recruit the transcription factors and RNA Polymerase that compose the pre- initiation complex. 
E)  Open the DNA template and then bind RNA Polymerase at the transcriptional initiation site.

Question 5

A plant cell exhibits defects in transcription of transfer RNA genes. The gene encoding which polymerase is likely to be mutated in this cell?

Accepted Answer

The answer of A plant cell exhibits defects in transcription...

Question 6

Eukaryotes have how many different RNA polymerases?

Accepted Answer

The answer of Eukaryotes have how many different RNA polymerases?...

Question 7

What is the consensus sequence for the Pribnow box from these sequences?

Accepted Answer

A)  TATGAT 
B)  TATTAT 
C)  TAGTAT 
D)  ACCA 
E)  TTGATA 
A)  TATGAT 
B)  TATTAT 
C)  TAGTAT 
D)  ACCA 
E)  TTGATA

Question 8

What are the three types of posttranscriptional processing, and what are the consequences of preventing each of these modifications in terms of gene expression?

Accepted Answer

(a) 5' capping: the addition of a modifi

Question 9

What are the two chemical structures found in RNA but not DNA?

Accepted Answer

ribose sug

Question 10

You wish to create a mutation that prevents access of RNA polymerase to the gene. Which region of a gene would you mutate?

Accepted Answer

A)  terminator sequence 
B)  stop codon 
C)  coding region 
D)  start codon 
E)  promoter sequence 
A)  terminator sequence 
B)  stop codon 
C)  coding region 
D)  start codon 
E)  promoter sequence

Question 11

You wish to prevent transcription of all three types of RNA in bacteria. How many different types of RNA polymerase would you need to inhibit?

Accepted Answer

The answer of You wish to prevent transcription of all...

Question 12

What are catalytically active RNAs that can activate processes such as self- splicing?

Accepted Answer

A)  snRNAs 
B)  ribosomes 
C)  pre- mRNAs 
D)  rnzymes 
E)  ribozymes 
A)  snRNAs 
B)  ribosomes 
C)  pre- mRNAs 
D)  rnzymes 
E)  ribozymes

Question 13

A gene has acquired a mutation in which the protein product has 50 additional amino acids at the end. Which region of the gene was likely mutated?

Accepted Answer

A)  coding region 
B)  stop colon 
C)  terminator sequence 
D)  promoter sequence 
E)  start codon 
A)  coding region 
B)  stop colon 
C)  terminator sequence 
D)  promoter sequence 
E)  start codon

Question 14

miRNA regulates protein production through which process?

Accepted Answer

The answer of miRNA regulates protein production through which process?...

Question 15

In humans, the 30S pre- RNA transcript yields three rRNA segments following transcription and RNA cleavage. Which RNA transcripts are generated by the 30S pre- RNA transcript in E. coli?

Accepted Answer

A)  two tRNA transcripts 
B)  three rRNAs (5S, 16S, and 23S) and two tRNA transcripts 
C)  three rRNAs (5S, 16S, and 23S) 
D)  three rRNAs (5.8S, 18S, and 28S) and two tRNA transcripts 
E)  two rRNAs (15S each) 
A)  two tRNA transcripts 
B)  three rRNAs (5S, 16S, and 23S) and two tRNA transcripts 
C)  three rRNAs (5S, 16S, and 23S) 
D)  three rRNAs (5.8S, 18S, and 28S) and two tRNA transcripts 
E)  two rRNAs (15S each)

Question 16

Which assay allows you to identify the exact location of the protein- binding sequence within a promoter?

Accepted Answer

A)  DNA footprint protection assay 
B)  pulse- chase assay 
C)  western/immuno blotting 
D)  in situ hybridization 
E)  Southern blotting 
A)  DNA footprint protection assay 
B)  pulse- chase assay 
C)  western/immuno blotting 
D)  in situ hybridization 
E)  Southern blotting

Question 17

What proteins bind to silencer sequences, forming a protein "bridge" that bends the DNA over the promoter and prevents transcription?

Accepted Answer

The answer of What proteins bind to silencer sequences, forming...

Question 18

Which of the following are present in your liver cells?

Accepted Answer

A)  enhancers of genes expressed in the kidney 
B)  promoters of genes expressed in the kidney 
C)  promoters of genes expressed in the liver 
D)  enhancers of genes expressed in the liver 
E)  All enhancers and promoters are present in liver cells 
A)  enhancers of genes expressed in the kidney 
B)  promoters of genes expressed in the kidney 
C)  promoters of genes expressed in the liver 
D)  enhancers of genes expressed in the liver 
E)  All enhancers and promoters are present in liver cells

Question 19

Why does rho- dependent transcriptional termination in bacteria require the rho protein?

Accepted Answer

A)  The rho protein assists in formation of the termination stem- loop that pauses the RNA Polymerase. 
B)  RNA Polymerase stalls at various sites in the gene and rho helps push RNA Polymerase to the end of the gene. 
C)  The rho protein helps unwind the stem- loop structure after the RNA has been released by RNA Polymerase. 
D)  The stem- loop is insufficiently stable to displace the RNA Polymerase by itself and needs rho protein to assist. 
E)  RNA Polymerase stalls on the termination stem- loop and rho is needed to displace the RNA Polymerase. 
A)  The rho protein assists in formation of the termination stem- loop that pauses the RNA Polymerase. 
B)  RNA Polymerase stalls at various sites in the gene and rho helps push RNA Polymerase to the end of the gene. 
C)  The rho protein helps unwind the stem- loop structure after the RNA has been released by RNA Polymerase. 
D)  The stem- loop is insufficiently stable to displace the RNA Polymerase by itself and needs rho protein to assist. 
E)  RNA Polymerase stalls on the termination stem- loop and rho is needed to displace the RNA Polymerase.

Question 20

Using the accompanying diagram, which of the following corresponds to the 3' untranslated region?

Accepted Answer

A)  A 
B)  B 
C)  C 
D)  G 
E)  F 
A)  A 
B)  B 
C)  C 
D)  G 
E)  F

Exam 8: Molecular Biology of Transcription and Rna Processing

What proteins aid in the recognition of the promoter sequence and binding of RNA polyme in eukaryotes?

Describe how band/mobility shift assays and DNA footprint protection assays can be used to identify promoter regions in DNA.

Intron self- splicing occurs when two________ reactions excise the intron and allow exons to ligate.

What must eukaryotes do to initiate transcription of a gene?

A plant cell exhibits defects in transcription of transfer RNA genes. The gene encoding which polymerase is likely to be mutated in this cell?

Eukaryotes have how many different RNA polymerases?

What is the consensus sequence for the Pribnow box from these sequences?

What are the three types of posttranscriptional processing, and what are the consequences of preventing each of these modifications in terms of gene expression?

What are the two chemical structures found in RNA but not DNA?

You wish to create a mutation that prevents access of RNA polymerase to the gene. Which region of a gene would you mutate?

You wish to prevent transcription of all three types of RNA in bacteria. How many different types of RNA polymerase would you need to inhibit?

What are catalytically active RNAs that can activate processes such as self- splicing?

A gene has acquired a mutation in which the protein product has 50 additional amino acids at the end. Which region of the gene was likely mutated?

miRNA regulates protein production through which process?

In humans, the 30S pre- RNA transcript yields three rRNA segments following transcription and RNA cleavage. Which RNA transcripts are generated by the 30S pre- RNA transcript in E. coli?

Which assay allows you to identify the exact location of the protein- binding sequence within a promoter?

What proteins bind to silencer sequences, forming a protein "bridge" that bends the DNA over the promoter and prevents transcription?

Which of the following are present in your liver cells?

Why does rho- dependent transcriptional termination in bacteria require the rho protein?

Using the accompanying diagram, which of the following corresponds to the 3' untranslated region?

The Molecular Basis of Heredity, Variation, and Evolution

Transmission Genetics

Cell Division and Chromosome Heredity

Inheritance Patterns of Single Genes and Gene Interaction

Genetic Linkage and Mapping in Eukaryotes

Genetic Analysis and Mapping in Bacteria and Bacteriophage

Dna Structure and Replication

The Molecular Biology of Translation

Chromosome Chapter Title Tbd

Gene Mutation, Dna Repair, and Homologous Recombination

Chromosome Chapter Title Tbd

Regulation of Gene Expression in Bacteria and Bacteriophage

Regulation of Gene Expression in Eukaryotes

Analysis of Gene Function by Forward Genetics and Reverse Genetics

Recombinant Dna Technology and Its Applications

Genomics: Genetics From a Whole-Genome Perspective

Organelle Inheritance and the Evolution of Organelle Genomes

Developmental Genetics

Genetic Analysis of Quantitative Traits

Population Genetics and Evolution at the Population, Species, and Molecular Levels

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