Exam 17: Recombinant Dna Technology

Reverse transcriptase is often used as the heat -stable enzyme in PCR.

Of what advantage is it to have a multiple cloning site (multiple unique restriction sites) embedded in the lacZ component of a plasmid?

Name at least two typical characteristics of a DNA cloning plasmid.

A restriction map provides the location of sites cleaved by restriction enzymes.

During a PCR, heat is provided to inactivate the polymerase enzyme.

Speculate on the origin of the restriction enzyme called EcoRI.

When propagating a clone in the lambda phage, would you have more immediate success if the phage entered the lysogenic cycle or the lytic cycle?

List two especially useful characteristics of cloning vectors.

In general, the main goal of cloning is to include as many different genes as possible in a single cloning vector.

A common term for a plasmid or other DNA element that serves as a cloning vehicle is vector.