Exam 19: Applications and Ethics of Genetic Engineering and Biotechnology

Under strictly controlled conditions, a probe can be used that will hybridize only with its complementary sequence and not with other sequences that may vary by as little as one nucleotide. What are such probes called?

Briefly describe what is meant by the term edible vaccine.

RFLPs are commonly used in recombinant DNA technology to _.

What makes "golden rice" golden?

A term often used to describe an organism that is a genetic mosaic, resulting from the introduction of DNA from another organism, is _.

Amniocentesis is preferred over chorionic villus sampling because recombinant DNA screening can be achieved with amniocentesis but not with chorionic villus sampling.

What is an allele -specific oligonucleotide?

The first person to receive gene therapy was a young girl with adenosine deaminase (ADA) deficiency. Outline the therapeutic steps involved.

Once DNA is separated on a gel, it is often desirable to gain some idea of its informational content. How might this be done?

How is a microarray used to scan for mutations in a genome?

Which term is commonly used to describe the production of valuable proteins in genetically modified plants and animals?

To generate glyphosate resistance in crop plants, a fusion gene was created that introduced a viral promoter to control the EPSP synthetase gene.

Alleles that differ by as little as a single nucleotide can be distinguished by synthetic probes known as _.

List the general genetic requirements and guidelines for gene therapy.

Which of the following characteristics do SNPs have?

Drosophila is a unique candidate for genetic engineering because it is the one prokaryote about which we have a great deal of genetic information.

The first attempts at gene therapy began in 1990 with the treatment of a young girl with a genetic disorder abbreviated SCID. What does SCID stand for? In the context of SCID, what does ADA stand for?