Exam 12: The Eukaryotic Chromosome

The technique of preparing mitotic chromosomes involves a staining procedure using Giemsa stain.One variation of this technique allows a low-resolution of chromosome bands while a second variation allows a high resolution.At low resolution we can see about ________ bands while at high resolution we see about ________ bands.

The single-celled yeast, Saccharomyces cerevisiae, was used to construct the first eukaryotic artificial chromosome.This was a significant achievement because

In yeast chromosomes, centromeres

By weight, chromatin consists roughly of

In mammalian cells, replication proceeds

The technique of preparing mitotic chromosomes involves a staining procedure using Giemsa stain.In this procedure, chromosomes are first ________ and then exposed to stain, producing ________ in a standard human karyotype.

When constructing a YAC, why must a large DNA insert be included?

What is true about new nucleosome formation during DNA replication?

What is the structure of telomeres?

Which of the combinations represents a complete yeast artificial chromosome (YAC)that is most likely to segregate correctly?

Select the statement that correctly describes the roles of histone and nonhistone proteins found in chromatin?

The enzyme that some organisms use to replicate DNA at the 5' ends of chromosomes is called

You treat a sample of DNA with DNase and run the digested fragments out on an electrophoretic gel.You found mostly long fragments.What can you conclude about this sample of DNA?

Methylated histone tail amino acids are associated with chromatin that is

You are studying a histone complex that contains an unusually high level of modification with acetyl groups.What can you conclude about the DNA bound to this histone complex?

The components of a chromosome include one long DNA molecule and

Sites of transcription and therefore most of the genes along the length of the chromosome appear to be found in

Which of the following predictions could you make about a cell in which the HP1 protein was disabled?

Which statement correctly describes the similarity of centromeres in different organisms?

FISH analysis is useful for determining the