Exam 8: Genetic Analysis and Mapping in Bacteria and Bacteriophages

A symbiotic relationship between a phage and a bacterium apparently occurs in the process of lysogeny.

Assume that the gene trpA in an auxotrophic strain of E.Coli is located at 27 minutes,whereas the gene pyrE is located at 81 minutes.

A form of bacterial recombination that involves a viral intermediate is called ________.

Name the general category into which double-stranded circular extrachromosomal DNA elements such as F factors,ColE1,and R would fall.

If two different auxotrophic strains are placed in a liquid medium culture tube,prototrophic strains can sometimes be subsequently recovered.Name several mechanisms by which this is possible.

What is a bacteriophage?

Distinguish between F⁺ and F^- bacteria.

Explain the composition and use of minimal medium in the study of bacterial genetics.

A bacteriophage that is capable of entering either a lytic or lysogenic cycle is called a(an)________.

The clearing made by bacteriophages in a "lawn" of bacteria on an agar plate is called a ________.

What is meant by the term cotransformation?

Describe how different strains of E.Coli can reveal different linkage arrangements of genes in Hfr crosses.

What is a form of recombination in bacteria that involves the F plasmid?

An Hfr cell can initiate chromosome transfer from one E.Coli to another.

Viral mutations and variants are often categorized by changes in host range and/or plaque morphology.

When a bacteriophage genome incorporates itself into the chromosome of the host,that phage genome is referred to as a(an)________.

What is the role of the F factor in bacterial recombination?

Name the typical phases of the bacterial growth cycle in liquid culture medium.

In what way was the interrupted mating technique used to generate a genetic map in E.Coli based on time?

Explain what is meant by the term heteroduplex in the context of bacterial transformation.