Question 1

Which of the following vectors is useful in producing single-stranded DNA?

Accepted Answer

A) puc18 
B) $\lambda$ phage 
C) M13 
D) BAC 
E) YAC 
A) puc18 
B) $\lambda$ phage 
C) M13 
D) BAC 
E) YAC

Question 2

What is the RACE technique?

Accepted Answer

A) screening a genomic library 
B) extending incomplete cDNA sequences 
C) generating polynucleotide probes 
D) rapid amplification of genomic DNA 
E) screening a genomic library and generating polynucleotide probes 
A) screening a genomic library 
B) extending incomplete cDNA sequences 
C) generating polynucleotide probes 
D) rapid amplification of genomic DNA 
E) screening a genomic library and generating polynucleotide probes

Question 3

The correct 10-base forward PCR primer starting at position 5 on the following sequence would be 5'-ATGCCGATGTAGGGCGGGATGGAGAGATAGAGAGAGTCACAAT-3'

Accepted Answer

A) 5-GGGATGGCAT 
B) 5'-ATGCCGATGT 
C) 5'-CGATGTAGGG 
D) 5'-GAGTCACAAT 
E) 5'-GCTACATCCC 
A) 5-GGGATGGCAT 
B) 5'-ATGCCGATGT 
C) 5'-CGATGTAGGG 
D) 5'-GAGTCACAAT 
E) 5'-GCTACATCCC

Question 4

Which of the following vectors is the best choice for the expression of eukaryotic proteins?

Accepted Answer

A) M13 
B) baculovirus 
C) phagemid 
D) pUC18 
E) pUC19 
A) M13 
B) baculovirus 
C) phagemid 
D) pUC18 
E) pUC19

Question 5

A new enzyme was isolated and found to have a recognition sequence of 5 base pairs.How often will it cut in a genome?

Accepted Answer

A) 64 
B) 4096 
C) 8192 
D) 1024 
E) 3124 
A) 64 
B) 4096 
C) 8192 
D) 1024 
E) 3124

Question 6

An enzyme that recognizes different sites in an identical sequence is called a ____________.

Accepted Answer

The answer of An enzyme that recognizes different sites in...

Question 7

A disadvantage of using a prokaryotic expression system for eukaryotic proteins is that the proteins are

Accepted Answer

A) highly phosphorylated after translation. 
B) improperly folded. 
C) highly soluble. 
D) heavily glycosylated. 
E) over expressed. 
A) highly phosphorylated after translation. 
B) improperly folded. 
C) highly soluble. 
D) heavily glycosylated. 
E) over expressed.

Question 8

In constructing a cDNA library,which of the following would you use to generate rare restriction sites on the cDNA strands?

Accepted Answer

A) restriction enzymes 
B) PCR with multiple cycles 
C) BamHI restriction followed by ligation 
D) ligate cDNA to specific linkers 
E) 5'RACE 
A) restriction enzymes 
B) PCR with multiple cycles 
C) BamHI restriction followed by ligation 
D) ligate cDNA to specific linkers 
E) 5'RACE

Question 9

Which of the following enzymes will produce blunt-ended cuts in DNA?

Accepted Answer

A) EcoRI 
B) HindIII 
C) SmaI 
D) BamHI 
E) PstI 
A) EcoRI 
B) HindIII 
C) SmaI 
D) BamHI 
E) PstI

Question 10

The use of high voltage to drive DNA into cells is called _____________.

Accepted Answer

The answer of The use of high voltage to drive...

Which of the following vectors is useful in producing single-stranded DNA?

What is the RACE technique?

The correct 10-base forward PCR primer starting at position 5 on the following sequence would be 5'-ATGCCGATGTAGGGCGGGATGGAGAGATAGAGAGAGTCACAAT-3'

Which of the following vectors is the best choice for the expression of eukaryotic proteins?

A new enzyme was isolated and found to have a recognition sequence of 5 base pairs.How often will it cut in a genome?

An enzyme that recognizes different sites in an identical sequence is called a ____________.

A disadvantage of using a prokaryotic expression system for eukaryotic proteins is that the proteins are

In constructing a cDNA library,which of the following would you use to generate rare restriction sites on the cDNA strands?

Which of the following enzymes will produce blunt-ended cuts in DNA?

The use of high voltage to drive DNA into cells is called _____________.

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Tools for Studying Genes and Gene Activity

The Mechanism of Transcription in Bacteria

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

Filters

Exam 4: Molecular Cloning Methods

Which of the following vectors is useful in producing single-stranded DNA?

What is the RACE technique?

The correct 10-base forward PCR primer starting at position 5 on the following sequence would be 5'-ATGCCGATGTAGGGCGGGATGGAGAGATAGAGAGAGTCACAAT-3'

Which of the following vectors is the best choice for the expression of eukaryotic proteins?

A new enzyme was isolated and found to have a recognition sequence of 5 base pairs.How often will it cut in a genome?

An enzyme that recognizes different sites in an identical sequence is called a ____________.

A disadvantage of using a prokaryotic expression system for eukaryotic proteins is that the proteins are

In constructing a cDNA library,which of the following would you use to generate rare restriction sites on the cDNA strands?

Which of the following enzymes will produce blunt-ended cuts in DNA?

The use of high voltage to drive DNA into cells is called _____________.

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Tools for Studying Genes and Gene Activity

The Mechanism of Transcription in Bacteria

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

Filters