Question 1

Chromatin remodeling usually occurs on euchromatin,while heterochromatin is very condensed and genetically inactive.

Accepted Answer

A) True 
 B)False  True

Question 2

Deacetylation of core histones allows for stronger binding of histones to DNA,which then inhibits ___________________.

Accepted Answer

transcription

Question 3

At least 20% of all amino acids found in histones are the basic amino acids

Accepted Answer

A) glycine or serine. 
B) arginine or lysine. 
C) tryptophane or tyrosine. 
D) valine or praline. 
E) cysteine or isoleucine. 
A) glycine or serine. 
B) arginine or lysine. 
C) tryptophane or tyrosine. 
D) valine or praline. 
E) cysteine or isoleucine. B

Question 4

On average,there is one nucleosme per _______bp of DNA.

Accepted Answer

The answer of On average,there is one nucleosme per _______bp...

Question 5

Histones have been shown to have an up regulatory effect on gene activity in vitro.

Accepted Answer

A) True 
 B)False

Question 6

Which of the following molecules can expedite RNA Pol II elongation through a nucleosome?

Accepted Answer

A) HAT 
B) FACT 
C) IFN-$\beta$ 
D) ChIP 
E) TRE 
A) HAT 
B) FACT 
C) IFN-$\beta$ 
D) ChIP 
E) TRE

Question 7

The energy necessary for chromatin remodeling comes from ATP.

Accepted Answer

A) True 
 B)False

Question 8

One method used by researchers to demonstrate the existence of nucleosome free DNA regions is

Accepted Answer

A) RNA Polymerase Run-off experiments. 
B) Nuclear Run on experiments. 
C) Reporter gene experiments. 
D) Anion Exchange chromatography. 
E) DNase hypersensitivity experiments. 
A) RNA Polymerase Run-off experiments. 
B) Nuclear Run on experiments. 
C) Reporter gene experiments. 
D) Anion Exchange chromatography. 
E) DNase hypersensitivity experiments.

Question 9

Layborne and Kadonage demonstrated in vitro that by adding increasing amounts of core histones and H1 they could repress transcription from the Drosophila Krüppel gene.

Accepted Answer

A) True 
 B)False

Question 10

Some transcription factors,such as ________ can act as both antirepressors and transcription activators.

Accepted Answer

A) GAL4 
B) Rad6 
C) SIR2 
D) RAP1 
E) HP1 
A) GAL4 
B) Rad6 
C) SIR2 
D) RAP1 
E) HP1

Question 11

If histone acetylation occurs in the cytoplasm,then histone deacetylation will also occur in the cytoplasm.

Accepted Answer

A) True 
 B)False

Question 12

When added to DNA in vitro,unacetylated histones tend to increase transcription from the 5S rRNA promoter.

Accepted Answer

A) True 
 B)False

Question 13

In nucleosome positioning,activators force the nucleosome to take up positions around the promoter so RNA polymerase can bind.

Accepted Answer

A) True 
 B)False

Question 14

Antirepressors such as GAGA factor are thought to compete with ________ for binding sites on the DNA template.

Accepted Answer

The answer of Antirepressors such as GAGA factor are thought...

Question 15

Histone acetyltransferase transfers acetyl groups from _______ to core histones.

Accepted Answer

The answer of Histone acetyltransferase transfers acetyl groups from _______...

Question 16

Which of the following histone proteins is the most highly conserved from one organism to another?

Accepted Answer

A) H1 
B) H2A 
C) H2B 
D) H3 
E) H4 
A) H1 
B) H2A 
C) H2B 
D) H3 
E) H4

Question 17

Chromatin immunoprecipitation (ChIP)can be used to study chromatin methylation in yeast cells.

Accepted Answer

A) True 
 B)False

Question 18

The Histone Code states that the

Accepted Answer

A) primary sequence of the histone proteins never changes over time. 
B) combination of histone modification on a given nucleosome near a gene's control region affects the efficiency of transcription of that gene. 
C) combination of histone modification on a given nucleosome near a gene's control region affects the efficiency of transcription of all the nearby genes. 
D) lysines are the only amino acids found in histones that can be acetylated and deacetylated. 
E) histones are found in all living cells. 
A) primary sequence of the histone proteins never changes over time. 
B) combination of histone modification on a given nucleosome near a gene's control region affects the efficiency of transcription of that gene. 
C) combination of histone modification on a given nucleosome near a gene's control region affects the efficiency of transcription of all the nearby genes. 
D) lysines are the only amino acids found in histones that can be acetylated and deacetylated. 
E) histones are found in all living cells.

Question 19

Which of the following is the most common type of histone modification?

Accepted Answer

A) lysine $\varepsilon$-amino methylation 
B) serine o-phosphorylation 
C) lysine n-phosphorylation 
D) histidine n-phosphorylation 
E) acetylation 
A) lysine $\varepsilon$-amino methylation 
B) serine o-phosphorylation 
C) lysine n-phosphorylation 
D) histidine n-phosphorylation 
E) acetylation

Question 20

Nucleosome remodeling can either activate or repress transcription.

Accepted Answer

A) True 
 B)False

Exam 13: Chromatin Structure and Its Effect on Transcription

Chromatin remodeling usually occurs on euchromatin,while heterochromatin is very condensed and genetically inactive.

Deacetylation of core histones allows for stronger binding of histones to DNA,which then inhibits ___________________.

At least 20% of all amino acids found in histones are the basic amino acids

On average,there is one nucleosme per _______bp of DNA.

Histones have been shown to have an up regulatory effect on gene activity in vitro.

Which of the following molecules can expedite RNA Pol II elongation through a nucleosome?

The energy necessary for chromatin remodeling comes from ATP.

One method used by researchers to demonstrate the existence of nucleosome free DNA regions is

Layborne and Kadonage demonstrated in vitro that by adding increasing amounts of core histones and H1 they could repress transcription from the Drosophila Krüppel gene.

Some transcription factors,such as ________ can act as both antirepressors and transcription activators.

If histone acetylation occurs in the cytoplasm,then histone deacetylation will also occur in the cytoplasm.

When added to DNA in vitro,unacetylated histones tend to increase transcription from the 5S rRNA promoter.

In nucleosome positioning,activators force the nucleosome to take up positions around the promoter so RNA polymerase can bind.

Antirepressors such as GAGA factor are thought to compete with ________ for binding sites on the DNA template.

Histone acetyltransferase transfers acetyl groups from _______ to core histones.

Which of the following histone proteins is the most highly conserved from one organism to another?

Chromatin immunoprecipitation (ChIP)can be used to study chromatin methylation in yeast cells.

The Histone Code states that the

Which of the following is the most common type of histone modification?

Nucleosome remodeling can either activate or repress transcription.

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

Molecular Tools for Studying Genes and Gene Activity

The Mechanism of Transcription in Bacteria

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

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