Question 1

Fluorescence in situ hybridization can be used to locate genes on individual chromosomes.

Accepted Answer

A) True 
 B)False

Question 2

Primer extension can be used to locate the exons in a transcript.

Accepted Answer

A) True 
 B)False

Question 3

Rapid purification of proteins based on size can best be done using

Accepted Answer

A) gel filtration. 
B) ion-exchange chromatography. 
C) native gel electrophoresis. 
D) 2-D gel electrophoresis. 
E) cation-exchange chromatography. 
A) gel filtration. 
B) ion-exchange chromatography. 
C) native gel electrophoresis. 
D) 2-D gel electrophoresis. 
E) cation-exchange chromatography.

Question 4

A technique is needed to rapidly separate positively charged histone proteins from a crude cell extract.Which of the following should be chosen?

Accepted Answer

A) SDS-PAGE 
B) phosphocellulose cation-exchange chromatography 
C) pulse-field gel electophoresis 
D) gel filtration chromatography 
E) DEAE-Sephadex chromatography 
A) SDS-PAGE 
B) phosphocellulose cation-exchange chromatography 
C) pulse-field gel electophoresis 
D) gel filtration chromatography 
E) DEAE-Sephadex chromatography

Question 5

Which of the following techniques is not used to quantify the level of gene expression?

Accepted Answer

A) southern blotting 
B) northern blotting 
C) S1 mapping 
D) RNase protection 
E) nuclear run-off 
A) southern blotting 
B) northern blotting 
C) S1 mapping 
D) RNase protection 
E) nuclear run-off

Question 6

A study was conducted to identify a liver cell gene (zed)that has a length of 3000 bp.A southern blot was prepared using DNA from the liver cell and a probe made to region 1600-2000 of zed.Analysis of the blot revealed that there are two spots present,one at 3000 and another at 2000.A plausible explanation for this observation is:

Accepted Answer

A) there is an identical copy of this gene on another chromosome. 
B) the gene was cut in two equal portions by a restriction enzyme. 
C) there is a another gene with sequence similar to gene zed. 
D) both there is an identical copy of this gene on another chromosome and there is a another gene with sequence similar to gene zed are correct. 
E) no explanation can be given using the available data. 
A) there is an identical copy of this gene on another chromosome. 
B) the gene was cut in two equal portions by a restriction enzyme. 
C) there is a another gene with sequence similar to gene zed. 
D) both there is an identical copy of this gene on another chromosome and there is a another gene with sequence similar to gene zed are correct. 
E) no explanation can be given using the available data.

Question 7

_____________ ________________ can be used to produce disrupted genes in several organisms.

Accepted Answer

Homologous

Question 8

The SELEX method is used for which of the following?

Accepted Answer

A) discover short RNA sequence that interact with other molecules 
B) assay transcriptional levels abundant aptamers 
C) evaluate transcribed regions of DNA 
D) discover new mutations in rRNA sequences 
E) none of the choices are correct. 
A) discover short RNA sequence that interact with other molecules 
B) assay transcriptional levels abundant aptamers 
C) evaluate transcribed regions of DNA 
D) discover new mutations in rRNA sequences 
E) none of the choices are correct.

Question 9

The G-less cassette assay is conducted using a promoter fused to a short DNA sequence and can be used to measure the transcription activity in a cell.

Accepted Answer

A) True 
 B)False

Question 10

During the first phase of two-dimensional gel electrophoresis,each polypeptide migrated based on its molecular mass.

Accepted Answer

A) True 
 B)False

Fluorescence in situ hybridization can be used to locate genes on individual chromosomes.

Primer extension can be used to locate the exons in a transcript.

Rapid purification of proteins based on size can best be done using

A technique is needed to rapidly separate positively charged histone proteins from a crude cell extract.Which of the following should be chosen?

Which of the following techniques is not used to quantify the level of gene expression?

_ ____ can be used to produce disrupted genes in several organisms.

The SELEX method is used for which of the following?

The G-less cassette assay is conducted using a promoter fused to a short DNA sequence and can be used to measure the transcription activity in a cell.

During the first phase of two-dimensional gel electrophoresis,each polypeptide migrated based on its molecular mass.

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

The Mechanism of Transcription in Bacteria

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

Filters

Exam 5: Molecular Tools for Studying Genes and Gene Activity

Fluorescence in situ hybridization can be used to locate genes on individual chromosomes.

Primer extension can be used to locate the exons in a transcript.

Rapid purification of proteins based on size can best be done using

A technique is needed to rapidly separate positively charged histone proteins from a crude cell extract.Which of the following should be chosen?

Which of the following techniques is not used to quantify the level of gene expression?

_____________ ________________ can be used to produce disrupted genes in several organisms.

The SELEX method is used for which of the following?

The G-less cassette assay is conducted using a promoter fused to a short DNA sequence and can be used to measure the transcription activity in a cell.

During the first phase of two-dimensional gel electrophoresis,each polypeptide migrated based on its molecular mass.

A Brief History

The Molecular Nature of Genes

An Introduction to Gene Function

Molecular Cloning Methods

The Mechanism of Transcription in Bacteria

Operons: Fine Control of Bacterial Transcription

Major Shifts in Bacterial Transcription

Dna-Protein Interactions in Bacteria

Eukaryotic Rna Polymerases and Their Promoters

General Transcription Factors in Eukaryotes

Transcription Activators in Eukaryotes

Chromatin Structure and Its Effect on Transcription

Rna Processing I: Splicing

Rna Processing II: Capping and Polyadenylation

Rna Processing: Other Post-Transcriptional Events

The Mechanism of Translation I: Initiation

The Mechanism of Translation II: Elongation and Termination

Ribosomes and Transfer Rna

Dna Replication,damage and Repair

Dna Replication II: Detailed Mechanism

Homologous Recombination

Transposition

Introduction to Genomics: Dna Sequencing on a Genomic Scale

Genomics II: Functional Genomics,proteomics and Bioinformatics

Filters

_ ____ can be used to produce disrupted genes in several organisms.