Question 1

Select all structural motifs that promote protein dimerization.

Accepted Answer

A)   Leucine zipper
B)   Helix-loop-helix
C)   Helix-turn-helix
D)   Zinc finger A, B

Question 2

Methylation of DNA bases is one way that regulatory transcription factors may influence gene expression.

Accepted Answer

A) True 
 B)False  False

Question 3

Insulators exert their effects on gene expression through histone acetyltransferase, histone deacetylase, and RNA polymerase.

Accepted Answer

A) True 
 B)False  False

Question 4

A repressor protein would enhance the ability of TFIID to bind to the TATA box of the promoter.

Accepted Answer

A) True 
 B)False

Question 5

A mutation in which of the following would result in little or no effect on the expression of a gene regulated by a CRE?

Accepted Answer

A)   G protein
B)   Adenylyl cyclase
C)   Protein kinase A
D)   CREB
E)   Glucocorticoid receptor

Question 6

CpG islands are associated with which of the following?

Accepted Answer

A)   Nucleosome location
B)   DNA methylation
C)   Steroid hormone activity
D)   cAMP pathway

Question 7

Genomic imprinting is a result of ________.

Accepted Answer

A)   Nucleosome location
B)   Histone activation
C)   DNA methylation
D)   Serine to leucine changes in the genetic code

Question 8

Activator proteins bind to silencer sequences and repressor proteins bind to enhancer sequences.

Accepted Answer

A) True 
 B)False

Question 9

What general transcription factor is most often affected by regulatory transcription factors?

Accepted Answer

A)   TFIIB
B)   TFIID
C)   TFIIE
D)   TFIIF

Question 10

DNA methylation usually activates gene expression.

Accepted Answer

A) True 
 B)False

Question 11

A heterodimer occurs when two identical transcription factors interact on a sequence of DNA.

Accepted Answer

A) True 
 B)False

Question 12

Housekeeping genes are unmethylated and active in most cells.

Accepted Answer

A) True 
 B)False

Question 13

Prior to hormone binding, receptors for steroid hormones are usually found in the nucleus of the cell.

Accepted Answer

A) True 
 B)False

Question 14

If a portion of a transcription factor's domain is the same in a variety of organisms, it is called a motif.

Accepted Answer

A) True 
 B)False

Question 15

DNA that contains actively transcribed genes would most likely contain chromatin in the closed configuration.

Accepted Answer

A) True 
 B)False

Question 16

Which of the following might complicate the interpretation of ChIP-Seq data?

Accepted Answer

A)   Because histones can be moved around or ejected from the chromatin, DNA that is only sometimes wrapped around a histone might not be sequenced.
B)   DNA wrapped around nucleosomes can never be sequenced.
C)   The regions of DNA wrapped around a histone is too long to be sequenced with current technologies.
D)   The DNA between nucleosomes are too compacted to be sequenced.

Question 17

Which DNA sequence would also work in place of the following bi-directional enhancer? 5' GTTC 3'
3' CAAG 5'

Accepted Answer

A)   5' GAAC 3' 3' CTTG 5'
B)   5' CTTG 3' 3' GAAC 5'
C)   5' CAAG 3' 3' GTTC 5'
D)   5' CTTC 3' 3' GAAG 5'

Question 18

A particular gene has a mutation in its NFR that causes it to bind abnormally tightly to histones. What effect would you expect this mutation has on the expression of this gene?

Accepted Answer

A)   The expression of the gene would be abnormally high.
B)   The effect on gene expression is impossible to predict.
C)   The gene would encode a different protein instead.
D)   The expression of the gene would be abnormally low or absent.
E)   The promoter of the gene will be constantly available to transcription factors and RNA polymerase.

Question 19

Nucleosome location may be changed by a process called ATP-dependent chromatin remodeling.

Accepted Answer

A) True 
 B)False

Question 20

In ChIP-Seq, the DNA fragments to be sequenced are around 150 bp in length. What does this size of DNA represent?

Accepted Answer

A)   The length of a promoter
B)   The amount of DNA wrapped around a nucleosome
C)   The average length of a gene
D)   The average distance between genes
E)   The amount of DNA between nucleosomes

Exam 15: Gene Regulation in Eukaryotes I: Transcriptional and Translation Regulation

Select all structural motifs that promote protein dimerization.

Methylation of DNA bases is one way that regulatory transcription factors may influence gene expression.

Insulators exert their effects on gene expression through histone acetyltransferase, histone deacetylase, and RNA polymerase.

A repressor protein would enhance the ability of TFIID to bind to the TATA box of the promoter.

A mutation in which of the following would result in little or no effect on the expression of a gene regulated by a CRE?

CpG islands are associated with which of the following?

Genomic imprinting is a result of ________.

Activator proteins bind to silencer sequences and repressor proteins bind to enhancer sequences.

What general transcription factor is most often affected by regulatory transcription factors?

DNA methylation usually activates gene expression.

A heterodimer occurs when two identical transcription factors interact on a sequence of DNA.

Housekeeping genes are unmethylated and active in most cells.

Prior to hormone binding, receptors for steroid hormones are usually found in the nucleus of the cell.

If a portion of a transcription factor's domain is the same in a variety of organisms, it is called a motif.

DNA that contains actively transcribed genes would most likely contain chromatin in the closed configuration.

Which of the following might complicate the interpretation of ChIP-Seq data?

Which DNA sequence would also work in place of the following bi-directional enhancer? 5' GTTC 3' 3' CAAG 5'

A particular gene has a mutation in its NFR that causes it to bind abnormally tightly to histones. What effect would you expect this mutation has on the expression of this gene?

Nucleosome location may be changed by a process called ATP-dependent chromatin remodeling.

In ChIP-Seq, the DNA fragments to be sequenced are around 150 bp in length. What does this size of DNA represent?