In Affinity Labeling,a Technique Used to Study Enzyme Mechanisms

In affinity labeling,a technique used to study enzyme mechanisms,

A) A compound designed to bind at the active site reacts with and therefore permits the identification of a nearby group.
B) A fluorescent group is attached to the substrate to identify the position of the active site.
C) Highly purified enzyme is obtained by affinity chromatography for mechanistic studies.
D) A radioactive isotope is incorporated into the substrate to identify the position of the active site.
E) Genetic modification of amino acid residues is used to alter the binding affinity of the active site.

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