Question 1

Give the sequence and draw the first two residues of a morpholino oligo that would serve as an antisense RNA for the following mRNA sequence (you may use the letter A, C, G and U to denote the bases):
5'-CAUGUCC-3'

Accepted Answer

5'-GGACAUG-5'

Question 2

DNA methylation in eukaryotes plays a role in ____________.

Accepted Answer

A)  methyl-directed mismatch repair
B)  protection of restriction sites
C)  chromatin remodeling
D)  cancerous cell growth
E)  gene silencing E

Question 3

At the transcriptional level, how does tryptophan control the production of genes within the trp operon?

Accepted Answer

A)  the trp repressor contains a high percentage of Trp residues and is thus only produced when Trp levels are high
B)  without sufficient Trp, the production of the trp repressor is truncated, thus producing a protein that cannot bind to the operon, ultimately resulting in structural gene expression
C)  high levels of Trp cause the repressor to be proteolytically digested
D)  the trp repressor binds to the operon only when bound to Trp
E)  none of the above D

Question 4

When bacteriophage $\lambda$ lysogeny terminates and lytic growth in E. coli cells begins, ____ is inactivated leading to unoccupied OR sites. _____ is produced, ultimately resulting in DNA replication.

Accepted Answer

A)  Cro; cI
B)  cI; Cro
C)  cII; Cro
D)  cI; cII
E)  none of the above

Question 5

What is thought to occur when CRP binds to DNA?

Accepted Answer

A)  formation of a hairpin that prevents progression of RNA polymerase
B)  bending of DNA to facilitate RNA polymerase binding
C)  blocking of promoter regions to prevent binding of RNA polymerase
D)  forms non-covalent interactions with RNA polymerase to bind it to DNA
E)  none of the above

Question 6

In a histone protein, what modification to lysine marks the nucleosome as a transcription target?

Accepted Answer

A)  phosphorylation
B)  methylation
C)  ubiquitylation
D)  ADP-ribosylation
E)  acetylation

Question 7

The phosphorylation of several RNA polymerase II serine residues has been identified as a key event in eukaryotic translation initiation. Which of the following is one of the enzymes responsible for this phosphorylation?

Accepted Answer

A)  Mediator complex
B)  Rap1
C)  TFIIH
D)  TATA box-binding protein
E)  ACT

Question 8

On the polycistronic mRNA of bacteriophage MS2, the ability to translate multiple copies of the coat protein but only a single copy of the A protein is accomplished by _________.

Accepted Answer

A)  regulatory RNA molecules
B)  translational repression by protein binding to mRNA
C)  antisense RNA
D)  ribosome binding site occlusion
E)  none of the above

Question 9

A short RNA that is complimentary to a specific mRNA and used for inhibition of translation is called ______________.

Accepted Answer

A)  antisense RNA
B)  missense RNA
C)  nonsense RNA
D)  senseless RNA
E)  none of the above

Question 10

Which of the genes in the SOS regulon encodes a DNA polymerase?

Accepted Answer

A)  recA
B)  lexA
C)  uvrA
D)  uvrB
E)  dinA

Question 11

If both lactose and glucose are present in large amounts, which of the following scenarios would result?

Accepted Answer

A)  lac repressor bound to promoter, CRP bound to promoter, no lac operon transcription
B)  lac repressor bound to inducer, CRP bound to promoter, no lac operon transcription
C)  lac repressor bound to inducer, CRP bound to promoter, lac operon transcribed
D)  lac repressor bound to inducer, CRP not bound, no lac operon transcription
E)  lac repressor bound to inducer, CRP not bound, lac operon transcribed

Question 12

DNA damage that requires RecA to bind to a DNA gap activates proteolysis of _____, which then activates the transcription of genes within the SOS regulon.

Accepted Answer

A)  cI
B)  Cro
C)  LexA
D)  uvrA
E)  uvrB

Question 13

An mRNA whose translation is controlled by binding of a metabolic end product such as flavin adenine dinucleotide or adenosylcobalamin is called a____________.

Accepted Answer

A)  ribozyme
B)  miRNA
C)  riboswitch
D)  regulatory RNA
E)  none of the above

Question 14

Which of the following bacteriophage $\lambda$ proteins serves as both an activator and a repressor?

Accepted Answer

A)  cI
B)  cII
C)  cIII
D)  Cro
E)  N

Question 15

Which of the following accounts for the greater complexity in eukaryotic transcriptional control when compared with prokaryotic control?

Accepted Answer

A)  physical separation of transcription from translation
B)  the presence of chromatin instead of naked DNA
C)  the larger size of the chromosomes
D)  the presence of introns
E)  none of the above

Question 16

Design an antisense RNA that prevents the start of translation for any mRNA in an E. coli cell.

Accepted Answer

The answer of Design an antisense RNA that prevents the...

Question 17

What elements are contained within the promoter of the lactose operon?

Accepted Answer

A)  operator and CRP site
B)  operator and repressor
C)  CRP site and repressor
D)  operator, CRP site and repressor
E)  none of the above

Question 18

Lysine methylation in a histone protein produces a form of chromatin known as ________ with the genes being transcriptionally __________.

Accepted Answer

A)  euchromatin; active
B)  euchromatin; inactive
C)  heterochromatin; active
D)  heterochromatin; inactive
E)  none of the above

Question 19

Bacteriophage $\lambda$ establishment of lysogeny in E. coli cells requires a high concentration of _____. This is achieved by the ____ protein binding to the PRE regulatory site.

Accepted Answer

A)  Cro; cI
B)  Cro; cII
C)  Cro; cIII
D)  cI; cII
E)  cI; cIII

Question 20

The bromodomain of nucleosome remodeling complexes binds to ____________ histones while the chromodomain binds to ___________ histones.

Accepted Answer

A)  acetylated; methylated
B)  acetylated; phosphorylated
C)  methylated; acetylated
D)  methylated; phosphorylated
E)  none of the above

Exam 29: Regulation of Gene Expression

Give the sequence and draw the first two residues of a morpholino oligo that would serve as an antisense RNA for the following mRNA sequence (you may use the letter A, C, G and U to denote the bases): 5'-CAUGUCC-3'

DNA methylation in eukaryotes plays a role in ____________.

At the transcriptional level, how does tryptophan control the production of genes within the trp operon?

When bacteriophage $\lambda$ lysogeny terminates and lytic growth in E. coli cells begins, ____ is inactivated leading to unoccupied O_R sites. _____ is produced, ultimately resulting in DNA replication.

What is thought to occur when CRP binds to DNA?

In a histone protein, what modification to lysine marks the nucleosome as a transcription target?

The phosphorylation of several RNA polymerase II serine residues has been identified as a key event in eukaryotic translation initiation. Which of the following is one of the enzymes responsible for this phosphorylation?

On the polycistronic mRNA of bacteriophage MS2, the ability to translate multiple copies of the coat protein but only a single copy of the A protein is accomplished by _________.

A short RNA that is complimentary to a specific mRNA and used for inhibition of translation is called ______________.

Which of the genes in the SOS regulon encodes a DNA polymerase?

If both lactose and glucose are present in large amounts, which of the following scenarios would result?

DNA damage that requires RecA to bind to a DNA gap activates proteolysis of _____, which then activates the transcription of genes within the SOS regulon.

An mRNA whose translation is controlled by binding of a metabolic end product such as flavin adenine dinucleotide or adenosylcobalamin is called a____________.

Which of the following bacteriophage $\lambda$ proteins serves as both an activator and a repressor?

Which of the following accounts for the greater complexity in eukaryotic transcriptional control when compared with prokaryotic control?

Design an antisense RNA that prevents the start of translation for any mRNA in an E. coli cell.

What elements are contained within the promoter of the lactose operon?

Lysine methylation in a histone protein produces a form of chromatin known as with the genes being transcriptionally __.

Bacteriophage $\lambda$ establishment of lysogeny in E. coli cells requires a high concentration of _. This is achieved by the protein binding to the P_RE regulatory site.

The bromodomain of nucleosome remodeling complexes binds to __ histones while the chromodomain binds to _ histones.

Exam 29: Regulation of Gene Expression

Give the sequence and draw the first two residues of a morpholino oligo that would serve as an antisense RNA for the following mRNA sequence (you may use the letter A, C, G and U to denote the bases): 5'-CAUGUCC-3'

DNA methylation in eukaryotes plays a role in ____________.

At the transcriptional level, how does tryptophan control the production of genes within the trp operon?

When bacteriophage λ\lambdaλ lysogeny terminates and lytic growth in E. coli cells begins, ____ is inactivated leading to unoccupied OR sites. _____ is produced, ultimately resulting in DNA replication.

What is thought to occur when CRP binds to DNA?

In a histone protein, what modification to lysine marks the nucleosome as a transcription target?

The phosphorylation of several RNA polymerase II serine residues has been identified as a key event in eukaryotic translation initiation. Which of the following is one of the enzymes responsible for this phosphorylation?

On the polycistronic mRNA of bacteriophage MS2, the ability to translate multiple copies of the coat protein but only a single copy of the A protein is accomplished by _________.

A short RNA that is complimentary to a specific mRNA and used for inhibition of translation is called ______________.

Which of the genes in the SOS regulon encodes a DNA polymerase?

If both lactose and glucose are present in large amounts, which of the following scenarios would result?

DNA damage that requires RecA to bind to a DNA gap activates proteolysis of _____, which then activates the transcription of genes within the SOS regulon.

An mRNA whose translation is controlled by binding of a metabolic end product such as flavin adenine dinucleotide or adenosylcobalamin is called a____________.

Which of the following bacteriophage λ\lambdaλ proteins serves as both an activator and a repressor?

Which of the following accounts for the greater complexity in eukaryotic transcriptional control when compared with prokaryotic control?

Design an antisense RNA that prevents the start of translation for any mRNA in an E. coli cell.

What elements are contained within the promoter of the lactose operon?

Lysine methylation in a histone protein produces a form of chromatin known as ________ with the genes being transcriptionally __________.

Bacteriophage λ\lambdaλ establishment of lysogeny in E. coli cells requires a high concentration of _____. This is achieved by the ____ protein binding to the PRE regulatory site.

The bromodomain of nucleosome remodeling complexes binds to ____________ histones while the chromodomain binds to ___________ histones.

When bacteriophage $\lambda$ lysogeny terminates and lytic growth in E. coli cells begins, ____ is inactivated leading to unoccupied O_R sites. _____ is produced, ultimately resulting in DNA replication.

Which of the following bacteriophage $\lambda$ proteins serves as both an activator and a repressor?

Lysine methylation in a histone protein produces a form of chromatin known as with the genes being transcriptionally __.

Bacteriophage $\lambda$ establishment of lysogeny in E. coli cells requires a high concentration of _. This is achieved by the protein binding to the P_RE regulatory site.

The bromodomain of nucleosome remodeling complexes binds to __ histones while the chromodomain binds to _ histones.