Capillary Columns Require Conditioning Prior to Their First Use

Capillary columns require conditioning prior to their first use.Typical conditioning is washing with 1 M NaOH for 1 hour,followed by 1 hour of water,then 1 hour of 6 M HCl,and finally 1 hour of run buffer.Which statements are INCORRECT?
I The NaOH wash is thought to generate the Si-OH groups on the silica surface.
II The NaOH wash removes any acidic contaminants from the silica surface.
III The HCl wash is thought to generate the Si-OH groups on the silica surface.
IV The HCl wash removes metal ions from the capillary surface.

A) I and III
B) I and IV
C) II and III
D) II and IV
E) I,II,and III

Correct Answer:

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