Multiple Choice
Which statement is NOT true for hydrophobic interaction chromatography?
A) Hydrophobic interaction chromatography has a hydrophobic stationary phase,which repels water and is not wetted by water.The technique is used primarily for protein purification.
B) Proteins are salted out of solution with ammonium,sodium,and potassium salts of phosphate and sulfate.Thiocyanate,iodide,and perchlorate salts increase the solubility of proteins in water.
C) The proteins are eluted from the column with a gradient of increasing salt concentration,increasing the solubility of proteins in water.
D) The most common stationary phase is an agarose gel with hydrophobic phenyl or alkyl groups attached and a pore size large enough to accommodate proteins.
E) A protein sample with a large concentration of ammonium sulfate is applied to the column.The ammonium sulfate causes the protein to stick to the hydrophobic surface of the stationary phase.
Correct Answer:
Verified
Correct Answer:
Verified
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