When Deciding Between an Isocratic Elution or a Gradient Elution,a

When deciding between an isocratic elution or a gradient elution,a scouting gradient should be done.Which of the criteria below is INCORRECT?

A) If t/t_G < 0.25,use isocratic elution.
B) If t/t_G > 0.40,use gradient elution.
C) For 0.25 < t/t_G < 0.40 use either an isocratic or gradient elution,the decision depending on factors such as available equipment or complexity of the sample.
D) For t/t_G < 0.25,a good starting isocratic elution solvent composition is the composition halfway through the t of the scouting gradient.
E) For 0.25 < t/t_G < 0.40,a good starting isocratic elution solvent composition is the composition halfway through the t_G of the scouting gradient.

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